Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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Micro-<strong>in</strong>sem<strong>in</strong>ation Device<br />
1. Removes r<strong>and</strong>omness of sperm/egg<br />
<strong>in</strong>teraction <strong>in</strong> conventional<br />
<strong>in</strong>sem<strong>in</strong>ation<br />
- size constra<strong>in</strong>t<br />
- di direct t ddelivery li of f sperm to t egg<br />
- recirculation or re-<strong>in</strong>sem<strong>in</strong>ation<br />
2. Unlike <strong>ICSI</strong>, it is non<strong>in</strong>vasive<br />
3. Potential <strong>in</strong>tegration with sperm sorter<br />
Suh et al., Human Reprod; 2006<br />
Fertilizationn<br />
Rate (%)<br />
50<br />
40<br />
30<br />
20<br />
10<br />
a<br />
n=324<br />
Micro-<strong>in</strong>sem<strong>in</strong>ation<br />
Center-well <strong>IVF</strong><br />
Microfluidic <strong>IVF</strong><br />
b<br />
n=104<br />
c<br />
n=147<br />
d<br />
n=378<br />
0<br />
Insem<strong>in</strong>ation Concentration (x 106 1 - 0.5 0.08 - 0.01<br />
)<br />
What is happen<strong>in</strong>g?<br />
At high sperm concentration:<br />
- high metabolic substrate<br />
usage<br />
- concentrated degradative<br />
by-product<br />
BAD for microfluidics<br />
a,b; c,d P
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