Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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IVF: A Multi-Step Process With Stepwise Inefficiencies Sperm Day 0 Egg gg Day 0 Insemination ICSI Day 0 Day 0 Fertilized Day 1 Optimization of each step → Greater overall success 4-Cell Embryo Day 2 8-Cell Embryo Day 3 Blastocyst Day 5 Microfluidics Ideal for Single Cells, Small Volumes & MicroPhysiology Could Microfluidics Be Useful In Isolation of Motile Sperm? Theory: In a microfluidic device, motile sperm would be able to deviate from their initial stream-of -flow, cross the inter-streamline, and be isolated and enriched. Initial stream-of-flow Inter Inter-streamline streamline Microscale Integrated Sperm Sorter Semen What was needed? Sperm injection port Fluid reservoir Sperm collection port Pump and/or power source “Vision” Out Out Sequentiial Embryo Culture Page 12 of 127
Polydimethylsiloxan and Sperm Survival Sperm Motility (%) a,b,c; P
Page 1: 28th Annual Meeting of the European
Page 5 and 6: Contents Course coordinators, cours
Page 7 and 8: Course coordinators Herman Tournaye
Page 9 and 10: Scientific programme Sperm selectio
Page 11 and 12: ESHRE SIG Andrology, Istanbul, Turk
Page 13: October 2001 - “A Physics Revelat
Page 17 and 18: Microfluidic Sperm Isolation Reduce
Page 19 and 20: Micro-insemination Device 1. Remove
Page 21 and 22: Selecting spermatozoa; potential ro
Page 23 and 24: Fertilization Rate (%) 120 100 80 6
Page 25 and 26: Spermsep CS10 System Sperm Cell Sep
Page 27 and 28: TUNEL (%) 14% 90 80 70 60 50 40 30
Page 29 and 30: Cathode (- ve terminal) Anode (+ ve
Page 31 and 32: Hydrogen peroxide mediates DNA dama
Page 33 and 34: ESHRE 2012: Pre‐congress course 2
Page 35 and 36: Sperm morphology and ICSI The estab
Page 37 and 38: Motile Sperm Organellar Morphology
Page 39 and 40: Conclusions of the meta‐analysis
Page 41 and 42: Does the presence of sperm nuclear
Page 43 and 44: Basic sperm parameters Sperm DNA fr
Page 45 and 46: No relationship between chromosome
Page 47 and 48: Gynecology: Filippo Ubaldi Elena Ba
Page 49 and 50: Sperm selection: why? There is an i
Page 51 and 52: I- MACS -Annexine V for diagnostic
Page 53 and 54: Evaluation of HA-binding and sperm
Page 55 and 56: HA-bound sperm and morphology with
Page 57 and 58: III. HA and ICSI outcome: prospecti
Page 59 and 60: HA- clinical uses and limitations
Page 61 and 62: May the force be with you Using imm
Page 63 and 64: Sperm ultrastructure Centrosome cha
Page 65 and 66:
Diagnostic semen analysis Three ba
Page 67 and 68:
Ultrastructural defects Transmissio
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Necrozoospermia / low viability rat
Page 71 and 72:
1. Hypo-osmotic swelling test ICSI
Page 73 and 74:
4. Laser-assisted selection (LAISS)
Page 75 and 76:
5. Birefringence-polarisation micro
Page 77 and 78:
Conclusions Absolute asthenozoospe
Page 79 and 80:
Definition of Azoospermia: Absence
Page 81 and 82:
Cases of aspermia Preventive measur
Page 83 and 84:
The probability crypto azoospermic
Page 85 and 86:
"The beauty and the beast" when the
Page 87 and 88:
Fertilization rate after ICSI 1- Oo
Page 89 and 90:
With (ICSI), zona pellucida penetra
Page 91 and 92:
Sibling oocytes from each patient w
Page 93 and 94:
The electron microscopic results in
Page 95 and 96:
Ionophore treatment for oocyte acti
Page 97 and 98:
The oocytes were suspended in 0.3M
Page 99 and 100:
Table 3. Pregnancy rates and outcom
Page 101 and 102:
References • Devroey P, Van Steir
Page 103 and 104:
Longing for a girl: Gender selectio
Page 105 and 106:
1,1 1 0,9 0,8 0,7 0,6 Sex ratio imb
Page 107 and 108:
PhD Thesis Michelle Duc, 1977 Retro
Page 109 and 110:
Reasons for wanting a girl at GC
Page 111 and 112:
Assessment of cervical mucus OOpaqu
Page 113 and 114:
Study design • A reference group
Page 115 and 116:
Partial data Reason n No post‐pre
Page 117 and 118:
Percentage P of girls 70 60 50 40 3
Page 119 and 120:
New aspects of this research • Fi
Page 121 and 122:
Mark your calendar for the upcoming
Page 123 and 124:
NOTES Page 121 of 127
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Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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