Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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<strong>IVF</strong>: A Multi-Step Process With Stepwise<br />
Inefficiencies<br />
Sperm<br />
Day 0<br />
Egg gg<br />
Day 0<br />
Insem<strong>in</strong>ation<br />
<strong>ICSI</strong><br />
Day 0<br />
Day 0<br />
Fertilized<br />
Day 1<br />
Optimization of each step → Greater overall success<br />
4-Cell Embryo<br />
Day 2<br />
8-Cell Embryo<br />
Day 3<br />
Blastocyst<br />
Day 5<br />
Microfluidics Ideal for S<strong>in</strong>gle Cells, Small Volumes & MicroPhysiology<br />
Could Microfluidics Be Useful In<br />
Isolation of Motile Sperm?<br />
Theory: In a microfluidic device, motile sperm would<br />
be able to deviate from their <strong>in</strong>itial stream-of<br />
-flow, cross the <strong>in</strong>ter-streaml<strong>in</strong>e, <strong>and</strong> be<br />
isolated <strong>and</strong> enriched.<br />
Initial stream-of-flow<br />
Inter Inter-streaml<strong>in</strong>e streaml<strong>in</strong>e<br />
Microscale Integrated Sperm Sorter<br />
Semen<br />
What was needed?<br />
Sperm <strong>in</strong>jection port<br />
Fluid reservoir<br />
Sperm collection port<br />
Pump <strong>and</strong>/or power<br />
source<br />
“Vision”<br />
Out<br />
Out<br />
Sequentiial<br />
Embryo Culture<br />
Page 12 of 127
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