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Cereals processing technology

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minimal protein level be maintained. Genetic engineering could allow a higher<br />

proportion of proteins in the malt either to contribute useful enzyme activity or<br />

support yeast nitrogen nutrition.<br />

4.6 Improved cereal quality control<br />

Bio<strong>technology</strong>, cereal and cereal products quality 65<br />

The value of cereals can be improved by better specification of the identity and<br />

composition of cereals in marketing. The major contributions to achieving this<br />

type of value addition are quality assurance programs and enhanced tools for<br />

analysis of products for chemical and microbial contamination and for genetic<br />

identity and purity.<br />

4.6.1 Chemical and microbial purity<br />

The strict application of quality assurance principles can ensure cereal safety<br />

and value. New technologies allow the use of ELISA tests to establish the level<br />

of pesticide residues and DNA-based analysis of microbial contaminants.<br />

4.6.2 Genetic purity<br />

The identity of a cereal genotype defines many of the quality characteristics<br />

such as protein content and grain size. This can define the <strong>processing</strong> value and<br />

optimal end use of a parcel of cereal grain. Protein content can currently be<br />

measured rapidly in the field using near infra-red reflectance (NIR). The<br />

development of rapid genotyping methods would find wide application in the<br />

cereal industry and allow a relatively complete objective description of samples<br />

for commercial valuation.<br />

Distinction of high-value noodle wheats from visually similar wheats of low<br />

noodle quality, and distinction of malting and food barleys with similar<br />

appearance, are good examples of the areas of potential application of this<br />

<strong>technology</strong>. Genetic purity and level of admixture are also important attributes<br />

to assess in commercial trading because of the financial incentive to add lowvalue<br />

grain to parcels of very high value but visually similar genotypes. Rapid<br />

DNA extraction from grain is a key technical requirement for successful<br />

application of these methods.<br />

4.7 Examples of transformed wheat and barley<br />

4.7.1 Desease resistance<br />

The first experiments to engineer resistance in barley focused on barley yellow<br />

dwarf virus. Wan and Lemaux (1994) transformed barley with a construct<br />

containing the coat protein of the virus under control of the constituent 35S<br />

promoter. This approach was based on the results of virus protection

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