Cereals processing technology
Cereals processing technology
Cereals processing technology
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56 <strong>Cereals</strong> <strong>processing</strong> <strong>technology</strong><br />
biotic factors may be engineered using appropriate genes. Resistance to viruses,<br />
bacteria, fungi, nematodes and insects has been reported.<br />
4.3.2 Manipulation of expression of native genes for disease resistance<br />
Classical cereal breeding has involved the combination of disease-resistant<br />
genes from different sources to produce commercial varieties with effective<br />
disease resistance (Hammondkosack and Jones 1997). Molecular markers or<br />
direct analysis for the presence of the required gene are now used to improve the<br />
efficiency of selection of disease-resistant lines in breeding.<br />
One option for the engineering of cereals with disease resistance is to<br />
manipulate or enhance the levels of expression of genes already present in the<br />
genome. Specific options include the expression of defence genes using<br />
constitutive promoters such that the defence gene product was always produced<br />
by the plant regardless of the presence of a specific pathogen. Promoters induced<br />
by the disease are also an important option. This approach allows defence gene<br />
products to be produced by the plant only in response to attack by the pest.<br />
4.3.3 Novel genes for disease resistance<br />
Novel genes from other plants or non-plant sources (Bowles 1990) may provide<br />
durable resistant genes for use in cereals (Table 4.2). Examples have been<br />
described for bacteria, fungi, nematodes and insects (Shewry and Lucas 1997).<br />
The use of virus-derived sequences for breeding virus-resistant plants has been a<br />
notable success in many species (Buck 1991, Malik 1999).<br />
4.3.4 Environmental impact of disease resistance<br />
Transgenic plants with resistance to pests and diseases may have a significant<br />
impact on the environment (Dale and Irwin 1998). The risks should be similar to<br />
Table 4.2 Some novel pest- and disease-resistant genes of potential value in cereals<br />
(Malik 1999)<br />
Type of Gene Source<br />
protection<br />
Virus Coat protection Barley yellow dwarf<br />
Coat protection Maize chlorotic mottle<br />
Maize chlorotic dwarf<br />
Maize chlorotic mosaic<br />
Bacterial Chitinase Various<br />
and fungi Glucanase Various<br />
Insects Bt toxin Bacillus thuringiensis