- Page 1 and 2: THE DIATOM PSEUDO-NITZSCHIA (PERAGA
- Page 3 and 4: i TABLE OF CONTENTS CONTENTS PAGE T
- Page 5 and 6: iii TABLE OF CONTENTS TABLES PAGE C
- Page 7 and 8: v TABLE OF CONTENTS FIGURES PAGE CH
- Page 9 and 10: vii TABLE OF CONTENTS FIGURES PAGE
- Page 11 and 12: ix TABLE OF CONTENTS PLATES PAGE CH
- Page 13 and 14: ACKNOWLEDGMENTS xi ACKNOWLEDGMENTS
- Page 15: OBJECTIVES OF THIS STUDY xiii OBJEC
- Page 19 and 20: 3 INTRODUCTION Figure 2. Axis and p
- Page 21 and 22: 5 INTRODUCTION Table 1. Nomenclatur
- Page 23 and 24: 1.2 TAXONOMY 1.2.1 MORPHOLOGY 7 INT
- Page 25 and 26: 9 INTRODUCTION Figure 3. Diagram sh
- Page 27 and 28: 11 INTRODUCTION Table 3. Distinct f
- Page 29 and 30: 13 INTRODUCTION Table 5. Additional
- Page 31 and 32: 15 INTRODUCTION Table 7. Features o
- Page 33 and 34: 17 INTRODUCTION Figure 5. Diagram s
- Page 35 and 36: 19 INTRODUCTION other domains are h
- Page 37 and 38: 21 INTRODUCTION using more conventi
- Page 39 and 40: Table 8. Global distribution of spe
- Page 41 and 42: 25 INTRODUCTION 1.4 DOMOIC ACID AND
- Page 43 and 44: 27 INTRODUCTION domoic acid (DA) wa
- Page 45 and 46: 29 INTRODUCTION seven Pseudo-nitzsc
- Page 47 and 48: 1.4.1 DOMOIC ACID 31 INTRODUCTION D
- Page 49 and 50: 33 INTRODUCTION One of the first ba
- Page 51 and 52: 35 INTRODUCTION Initially, a lot of
- Page 53 and 54: 37 INTRODUCTION P. multiseries was
- Page 55 and 56: 39 INTRODUCTION pungens f. multiser
- Page 57 and 58: 41 INTRODUCTION Dickman, M. and Gle
- Page 59 and 60: 43 INTRODUCTION Hallegraeff, G.M. A
- Page 61 and 62: 45 INTRODUCTION Impellizzeri, G., M
- Page 63 and 64: 47 INTRODUCTION Mann, D. G. 1978. S
- Page 65 and 66: 49 INTRODUCTION Reap, M.E. 1991. Ni
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51 INTRODUCTION Phytoplankton, Leaf
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53 INTRODUCTION Tryphonas, L., True
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CHAPTER 2
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ABSTRACT 56 CHAPTER 2 The presence
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58 CHAPTER 2 offshore. Occasional s
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Latitude (N) 57 56 55 54 53 52 51 E
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62 CHAPTER 2 Table 1. Morphometric
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Plate 1. Pseudo-nitzschia pungens (
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Plate 3. Pseudo-nitzschia australis
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Plate 4. Pseudo-nitzschia fraudulen
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Plate 5. Pseudo-nitzschia delicatis
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Plate 6. Pseudo-nitzschia pseudodel
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Plate 7. Pseudo-nitzschia cf. seria
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76 CHAPTER 2 Spatial and Temporal d
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Latitude (N) Latitude (N) 55.0 54.0
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80 CHAPTER 2 if the specimens exami
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82 CHAPTER 2 over P. multiseries le
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84 CHAPTER 2 can be mixed across th
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86 CHAPTER 2 Hasle, G.R. 1993. Nome
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CHAPTER 3
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ABSTRACT 89 CHAPTER 3 The presence
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91 CHAPTER 3 In the Bay of Fundy (E
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Latitude (N) Latitude (N) 53.0 52.5
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Latidtude (N) 53.0 52.5 52.0 51.5 M
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97 CHAPTER 3 Quantitative phytoplan
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h (m) 0 50 100 150 200 250 0 50 100
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101 CHAPTER 3 1313 (12m), “P. ser
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103 CHAPTER 3 Figure 8. Distributio
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cells.mL -1 105 Φ (J.m -3 ) 0 50 1
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107 CHAPTER 3 up to a maximum of 7.
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109 CHAPTER 3 Figure 13. Vertical p
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111 CHAPTER 3 Figure 14. Vertical p
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113 CHAPTER 3 The “P. delicatissi
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115 CHAPTER 3 Figure 18. Vertical p
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Salinity Salinity Salinity 35.5 35.
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119 CHAPTER 3 Figure 22. Temperatur
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h (m) 0 50 100 150 200 0 50 100 150
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123 CHAPTER 3 layer were low, betwe
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125 CHAPTER 3 Figure 27. Distributi
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127 CHAPTER 3 Table 1. Ranges in th
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129 CHAPTER 3 Figure 30. Areas of a
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Salinity 36.0 35.5 35.0 34.5 34.0 3
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DISCUSSION 133 CHAPTER 3 Pseudo-nit
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135 CHAPTER 3 greater diversity and
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137 CHAPTER 3 spp. have a tendency
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139 CHAPTER 3 thermocline is genera
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141 CHAPTER 3 are solely based on t
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REFERENCES 143 CHAPTER 3 Bates, S.S
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145 CHAPTER 3 genus Pseudo-nitzschi
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147 CHAPTER 3 Míguez, Á., Fernán
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149 CHAPTER 3 Roden, C.M., Lennon,
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151 CHAPTER 3 Walz, P.M., Garrison,
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152 CHAPTER 4 CONFIRMATION OF DOMOI
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INTRODUCTION 154 CHAPTER 4 The penn
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156 CHAPTER 4 for the unknown and a
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158 CHAPTER 4 with nebulizer-assist
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160 CHAPTER 4 Cultures 1 - 4 (under
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162 CHAPTER 4 Plate 1. Pseudo-nitzs
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Cells•mL -1 (x 10 3 ) 1000 100 10
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DISCUSSION 166 CHAPTER 4 Diatoms of
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168 CHAPTER 4 many studies have sho
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170 CHAPTER 4 Bates, S. S., Garriso
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172 CHAPTER 4 Hasle, G. R. 1965. Ni
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174 CHAPTER 4 Roden, C. M., Lennon,
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CHAPTER 5
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ABSTRACT 177 CHAPTER 5 Ribosomal DN
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179 CHAPTER 5 fact, in New Zealand,
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181 CHAPTER 5 Gel purification of t
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183 CHAPTER 5 Maximum-Parsimony tre
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185 CHAPTER 5 Table 3. LSU rRNA-tar
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Phylogenetic analysis 187 CHAPTER 5
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189 CHAPTER 5 Plate 1. Scanning ele
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Figure 1 continued. 191 CHAPTER 5
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193 CHAPTER 5 Figure 1 continued. 1
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195 CHAPTER 5 Figure 3. Phylogeneti
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197 CHAPTER 5 Application of rRNA-t
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DISCUSSION 199 CHAPTER 5 Since trad
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201 CHAPTER 5 probes (Scholin et al
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203 CHAPTER 5 The fluorescently lab
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205 CHAPTER 5 If the probes are to
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207 CHAPTER 5 Maidak B. L., Cole J.
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CONCLUDING REMARKS
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210 CONCLUDING REMARKS species were
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212 CONCLUDING REMARKS eastern Prin
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214 CONCLUDING REMARKS California.
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APPENDIX 1
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216 TERMINOLOGY TERMINOLOGY IN ACCO
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218 TERMINOLOGY TERMINOLOGY IN ACCO
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APPENDIX 2
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Pseudo-nitzschia pungens (Robust: v
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Pseudo-nitzschia multiseries (Robus
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Pseudo-nitzschia seriata f. seriata
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Pseudo-nitzschia seriata f. obtusa
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229 Pseudo-nitzschia australis g) T
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231 Pseudo-nitzschia fraudulenta th
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ELECTRON MICROSCOPY (EM) ACID CLEAN
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235 Pseudo-nitzschia heimii f) A ce
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237 Pseudo-nitzschia delicatissima
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239 Pseudo-nitzschia pseudodelicati
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Appendix III 240 APPENDIX III Micro
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Figs. 1-18 LIGHT MICROGRAPHS (LM).
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Figs. 1-18 Cont'd. Figs.1-17 Cont'd
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Figs. 19-40 Cont'd. Figs. 26-30 Con
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Fig. 41-55 TRANSMISSION ELECTRON MI
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Pseudo-nitzschia australis (Figures
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Figs. 56-86 Cont'd. Figs. 56-82 Con
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Figs. 56-86 Cont'd. Figs. 56-82 Con
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Figs. 56-86 Cont'd. LIGHT MICROGRAP
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Figs. 87-105 SCANNING ELECTRON MICR
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Figs. 106-154 TRANSMISSION ELECTRON
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Figs. 106-154 TRANSMISSION ELECTRON
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Figs. 106-154 TRANSMISSION ELECTRON
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Figs. 106-154 TRANSMISSION ELECTRON
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Figs. 155-168 LIGHT MICROGRAPHS (LM
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270 Pseudo-nitzschia fraudulenta Fi
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Figs. 169-210 Cont'd. Figs. 178-181
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Figs. 169-210 Cont'd. 274 Pseudo-ni
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Figs. 199-226 Cont'd. TRANSMISSION
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Figs. 199-226 Cont'd. TRANSMISSION
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Figs. 228-236 SCANNING ELECTRON MIC
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Pseudo-nitzschia delicatissima (Fig
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Figs. 237-247 Cont'd. LIGHT MICROGR
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Figs. 250-261 SCANNING ELECTRON MIC
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Figs. 262-334 Cont'd. TRANSMISSION
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Figs. 262-334 Cont'd. TRANSMISSION
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Figs. 262-334 Cont'd. TRANSMISSION
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Figs. 262-334 Cont'd. TRANSMISSION
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Figs. 262-334 Cont'd. TRANSMISSION
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Figs. 262-334 Cont'd. TRANSMISSION
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Figs. 335-351 SCANNING ELECTRON MIC
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Figs. 335-351 Cont'd. SCANNING ELEC
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304 Pseudo-nitzschia cf. multiserie
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Figs. 365-377 TRANSMISSION ELECTRON
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Pseudo-nitzschia cf. seriata (Figur
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Figs. 378-386 Cont'd. TRANSMISSION
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Appendix IV 311 APPENDIX IV A list
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313 CLEANING METHODS activity. Clea
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CHEMICAL CLEANING 315 CLEANING METH
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HYDROCHLORIC ACID AND POTASSIUM PER
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319 CLEANING METHODS Several ranges
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321 CLEANING METHODS PREPARATION OF
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Appendix V 322 APPENDIX V Short des
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324 PHYLOGENETICS pyrimidines, C an
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326 PHYLOGENETICS Figure 1. Multipl
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Figure 1 continued. 328 PHYLOGENETI
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Figure 1 continued. 330 PHYLOGENETI
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332 PHYLOGENETICS Figure 2. Multipl
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Figure 2 continued. 334 PHYLOGENETI
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Figure 2 continued. 336 PHYLOGENETI
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Figure 2 continued. 338 PHYLOGENETI
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Figure 2 continued. REFERENCES 340
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APPENDIX 6
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243 Pseudo-nitzschia pungens Cell n
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245 Pseudo-nitzschia pungens No. of
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347 Pseudo-nitzschia cf. seriata f.
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349 Pseudo-nitzschia australis Cell
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351 Pseudo-nitzschia australis Cell
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353 Pseudo-nitzschia fraudulenta Ce
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355 Pseudo-nitzschia cf. subpacific
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357 Pseudo-nitzschia delicatissima
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APPENDIX 7
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360 STATION DATA Table 1. Station p
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362 STATION DATA Table 3. Station p
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364 STATION DATA Table 5. Station p
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366 STATION DATA Table 7. Values fo
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368 STATION DATA Table 9. Values fo
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370 STATION DATA Table 11. Nutrient
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372 STATION DATA Table 13. Nutrient
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374 STATION DATA Table 15. Nutrient
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376 STATION DATA Figure 1. Vertical
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378 STATION DATA Table 17. Pseudo-n
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380 STATION DATA Table 21. Pseudo-n