Bwa-yo - Société Audubon Haiti
Bwa-yo - Société Audubon Haiti
Bwa-yo - Société Audubon Haiti
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138 Nim<br />
Table 16.1 Proximate analysis (% dry weight) of A. indica, after Gohl(1975).<br />
COMPONENT CRUDE CRUDE CRUDE CARBO- ASH Ca P<br />
PROTEIN FIBER FAT HYDRATES<br />
Fresh leaves (India) 15.4 12.7 4.2 56.5 11.2 2.65 0.24<br />
Fresh leaves (Pakistan) 13.4 14.7 6.2 55.5 10.3 1.94 0.17<br />
leucostica or C. meliae (Tourigny, 1987), under humid or poorly-ventilated conditions;<br />
a "carrot top" foliar disease ofuncertain cause; and leaf chlorosis caused by using potting<br />
medium contaminated with nematodes (Josiah, 1989; Runion et aI., 1990).<br />
Seedlings normally require 14 weeks in a Rootrainer or Winstrip prior to outplanting,<br />
with the initial 4 weeks under shade and the last 4 weeks reserved for hardening off.<br />
There are several alternative propagation methods that are less costly and simpler<br />
than the containerized seedling. The two methods that are the most practical for neem<br />
is stump propagation and the transplanting ofvolunteers from beneath selected mother<br />
trees. Stumps are prepared by raising seedlings directly in a raised bed and pruning both<br />
stem and roots prior to outplant. Reid (1991) .showed· no differences in survival or<br />
growth between stumps and containerized seedlings at 2 sites in <strong>Haiti</strong> following a year<br />
of growth. Volunteers are lifted from beneath selected mother trees and transplanted<br />
during the rains. A portion of the lifted seedlings are rejected because of natural root<br />
deformities associated with looping and poor vigor. One study revealed that up to 22%<br />
of the volunteers had root deformities, while 39% had excellent taproot formation<br />
(Larson et aI., 1985). While transplanting volunteers increases mortality rates and poorer<br />
root development, the trade-off has to be measured in terms of the economics and<br />
practicality'ofmanaging a containerized nursery under typical <strong>Haiti</strong>an farm conditions.<br />
Seed Handling: One of the most serious limitations in artificial propagation is the<br />
problem ofseed longevity and adequate germination procedures to test seed viability.<br />
Howeve.r, neem seed may be stored successfully up to 4 months if the seed is dried<br />
immediately following harvest in the sun for 3 days and stored in cotton bags at 15° C<br />
at reduced levels ofhumidity (Chaisurisri, 1986). Seed can be stored for longer periods<br />
of time if moisture content is lowered to 6.6-7.3% (dry weight basis) and stored continuously<br />
in sealed containers at 4°C. Furthermore, dormancy factors associated with<br />
the seed hull must be overcome to maximize germination capacity. Removal ofthe seed<br />
coat has shown a 10-fold increase in germination ofstored seed in <strong>Haiti</strong> (Timyan, 1991).<br />
Similar results were shown for 2 seed lots from Africa: 2-year-old seed germinated 24%<br />
compared to 62% with the endocarp removed, while 8 1h-year-old seed exhibited differences<br />
of 20% and 70% (Bellefontaine and Audinet, 1993).<br />
Azadirachtin Levels: Azadirachtin levels in neem seed were analyzed for tree andseed<br />
maturity effects in 1989 (Timyan and Walter, 1990). This study was initiated based on<br />
reports from W.R. Grace & Co. that certain seed lots from Africa tested 2- to 10-fold<br />
higher than seed lots from <strong>Haiti</strong>. It was suspected that both genetic and environmental<br />
effects contributed to this difference. No differences were detected between 3 levels of<br />
seed maturity, ranging from green seed on the tree and fallen seed on the ground.<br />
However, differences were detected among trees, sites, and seed collected during different<br />
seasons. There is reason to believe that even with the narrow genetic base that is