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experiment. The studies were ended after 32 days. Seeds not germinating by day 32 were considered germinated on day 33. Data were analyzed as means for each treatment combination (petri plate) using the Means, Correlation, and ANOVA procedures of SAS (SAS Institute). Results. The first experiment was designed to determine optimum temperature for cold germination tests. Maximum germination percentage (84 and 83%) was observed at 22 and 18°C, respectively (Table 1). The minimum germination percentage (44%) was observed at 14°C. In the first experiment, 'Charleston Gray' had 71 % germination compared to 64% for 'Petite Sweet'. The second experiment was to screen a diverse set of cultivars for germination speed at the chosen temperatures. In this experiment, 44 cultivars were tested at two different temerature, 14 and 18°C in the growth chamber. The maximum germination percentage (100%) was obtained for 17 cultivars: Blackstone, AU-Golden Producer, AU-Sweet Scarlet, Big Crimson, Black Diamond Yellow Belly, Calhoun Gray, Calsweet, Desert King, Fiesta, Klondike Striped II, Mickylee, Minilee, Peacock Shipper, Sugar Baby, Sultan F1, Super Gold, and Crimson Sweet (Table 2). Black Diamond Yellow Belly and Mickylee had high percentage germination (100%) at 18°C, but very low (0 to 5%) at 14°C. At 14°C, 'Blackstone' and 'Starbrite' had the highest germination percentage (90 to 95%), while the other cultivars were below 80% (Table 2). Low germination percentage at low temperature has been reported in cucumber at 14°C (Harrington and Minges, 1954; Hegarty, 1977). The watermelon cultivars used in this study were able to germinate at 14°C, but not at 10°C, during the 30 days used for the testing method. Some cultivars germinated rapidly, and with a high percentage at 14°C. If the trait is heritable, it could be transferred into elite cultivars to provide growers with more safety in early spring plantings. Improved cold germination ability may also provide better germination of triploid hybrids. In the future, a larger collection of germplasm should be screened for germination at low temperature. Cucurbit Genetics Cooperative Report 24:59-64 (2001) Also, temperatures between 10 and 14°C should be evaluated to determine whether the best watermelon accessions can germinate below 14°C. Literature Cited 1. Downs, R.J. and J.F. Thomas. 1983. Phytotron Procedural Manual for Controlled Environment Research at the Southeastern Plant Environment Laboratory. NCARS Tech. Bul. 244 (revised), 44 p. 2. Ellis, R.H., T.D. Hong, and E.H. Roberts. 1985. Handbook of seed technology for genebanks, volume II, pp. 317-327. Internat. Board Plant Genet. Resources, Rome, Italy. 3. Hall, M.R., S.R. Ghate, S.C. Phatak. 1989. Germinated seeds for field-establishment of watermelon. HortScience 24:236-238. 4. Harrington, J.F. and Minges, P.A. 1954. Vegetable seed germination. Univ. Calif. Agric. Ext. Serv. (Mimeo). 5. Hegarty, J.W. 1973. Temperature relations of germination in the field. In: W. Heydecker (Editor), Seed Ecology, Ch. 23 Proc. 19 th Easter school Agric. Sci. Univ. Nottingham, 1972. Butterworths, London, pp.411-432. 6. Hegarty, J.W. 1979. Factors influencing the emergence of calabrese and carrot seedlings in the field. J. Hort. Sci. 54: 194-207. 7. Jennings, P. and M. E. Saltveit. 1994. Temperature effects on imbibition and germination of cucumber (Cucumis sativus) seeds. J. Amer. Soc. Hort. Sci. 119: 464-467. 8. Khan, A.A., Karssen, C.M., Leve, E.F. and Roe, C.H. 1979. Preconditioning of seeds to improve performance. In T.K. Scott (Editor). Plant regulation and world Agriculture, Plenum Press, New York, pp. 395-414. 9. Kotowski, F. 1962. Temperature relations to germination of veg. Seeds Proc. Am. Soc. Hort. Sci. 23:176-184. 10. Milotay, P.L. Kovacs, and A. Barta. 1991. The effect of suboptimum temperatures on cucumber seed germination and seedling growth. Zoldsetermesztesi Kuutato Intezer Bullentinje 24: 33-45. 63
11. Mozumder, B.K.G, N.E. Caroselli, and L.S. Albert. 1970. Influence of water activity, temperature, and their interaction on germination of Verticillium albo-atrum conidia. Plant Physiol. 46: 347-349. 12. Nienhuis, J., Lower, R.L., and J. Staub. 1983. Selection for improved low temperature germination in cucumber (Cucumis sativus L.). J. Amer. Soc. Hort. Sci. 108: 1040-1043. 13. Nilsson, C. 1968. Temperature and germination in Cucumis sativus L. Agri. Hort. Genet. 25: 161-168. 14. Roeggen, O. 1987. Variation in minimum germination temperature for cultivars of bean (Phaseolus vulgaris L.), cucumber (Cucumis sativus L) and tomato (Lycopersicon esculentum Mill.) Scientia Horticulturae 33: 57-65. 15. Sachs, M. 1977. Priming of watermelon seeds for low temperature germination. HortScience 102: 175-178. 16. SAS Institute Inc. 1988. SAS/STAT user's guide, release 6.03 edition. Cary, NC 27512- 8000, USA. Cucurbit Genetics Cooperative Report 24:59-64 (2001) 17. Seget, A.V. 1966. The effect of temperature and properties of the soil substrate on the emergence of cucumber. Rostl. Vyroba.,12:317-334 (English summary). 18. Simon, E.W., M.C. Minchin, M.M. McMenamin, and Smith, J.M. 1976. The low temperature limit for seed germination. New Phytol. 77: 301- 311. 19. Staub, J.E., T.C. Wehner, and G.E. Tolla. 1989. The effect of chemical seed treatments on horticultural characteristics in cucumber (Cucumis sativus L). Scientia Hort. 38:1-10. 20. Thomas, T.H. 1981. Seed treatment and techniques to improve germination. Scientia Hort. 32:47-59. 21. Thompson, P.A. 1974. Effect of fluctuating temperature on germination. J. Exp. Bot., 25:164- 175. 22. U.S. Department of Agriculture. 1998. Agricultural statistics. U.S. Government Printing Office, Washington D.C. 64
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Cucurbit Genetics Cooperative Coope
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The Cucurbit Genetics Cooperative (
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this would be a welcome benefit to
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Georgia, Statesboro, GA, “Evaluat
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http://www.ashs.org/ashspress/ wate
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Cucurbit Genetics Cooperative Finan
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Table 1. The composition of culture
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Prezygotic barriers have previously
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Cucumber Inbred Line USDA 6632E Jac
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Multiple Shoot Induction from the S
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Table 3. Effects of combinations of
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Page 29 and 30: Figure 1. The viability of Cucumis
Page 31 and 32: Gynogenesis in a Dihaploid Line of
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Page 37 and 38: Fusarium Wilt Resistance in Eight I
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Page 43 and 44: of the loci (e.g., M7675, U13831, A
Page 45 and 46: Table 1. Percentage of RAPD band pr
Page 47 and 48: Inodorus Primer Casaba Honeydew Cha
Page 49 and 50: Frequency of RAPD Polymorphisms in
Page 51 and 52: BC551 76.6 92.9 38.5 20.5 38.9 42.2
Page 53 and 54: Powdery Mildew: An Emerging Disease
Page 55 and 56: Table 1 (continued).z Cultivar Coun
Page 57 and 58: Disease Severeity Rating Figure 1.
Page 59 and 60: In the present study C. lanatus ent
Page 61 and 62: Lakes, NJ) was a 0.5 cc (50 unit) s
Page 63 and 64: Characterization of a New Male Ster
Page 65 and 66: Table 1. Progeny test of male steri
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Page 69 and 70: elatively small during its developm
Page 71 and 72: content had no significant effect o
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Page 77 and 78: Table 1. Vine length of watermelon
Page 79 and 80: Survey of Watermelon Trialing Metho
Page 81 and 82: assigned to adjacent plots (Table 2
Page 83 and 84: Variation among tropical pumpkin cu
Page 85 and 86: Table 1. Aggressiveness of powdery
Page 87 and 88: A large variability of aggressivene
Page 89 and 90: Table 1: Observed reaction of melon
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Page 93 and 94: Relationship between Fruit Shape an
Page 95 and 96: Table 3. Seed yield per fruit and s
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Page 99 and 100: Lee et al. (17) developed a RAPD ma
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Page 105 and 106: Genetic Variability in Pumpkin (Cuc
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Page 115 and 116: Table 1. Components used for cultur
Page 117 and 118: 6. Jia, Shi-rong, You-ying Fu and Y
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Page 121 and 122: Gene List 2001 for Cucumber Jiahua
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de I determinate habit. Short vine
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gi - ginkgo. Leaves reduced and dis
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Mdh-4 Mdh- 3 Malate dehydrogenase-4
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h from 'Gy 2 cp cp', 'Spartan Salad
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wf - White flesh. Intense white fle
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M16056 Cotyledon cDNA Encoding ribu
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(for CsPK2.2) AB001590 Hypocotyl RN
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Caruth, T. F. 1975. A genetic study
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John, C. A. and J. D. Wilson. 1952.
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Ohkawa, J., A. Shinmyo, M. Kanchana
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Shimizu, S., K. Kanazawa, and A. Ka
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Youngner, V. B. 1952. A study of th
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Cucurbit Genetics Cooperative 2001-
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Denlinger, Phil Mt. Olive Pickle Co
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Keita, Sugiyama Kurume Branch, Natl
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germplasm evaluation and conservati
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Sarfatti, Matti Hazera Ltd., Resear
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Alabama Fenny Dane Arkansas Ted Mor
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Covenant and By-Laws of the Cucurbi
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ARTICLE IX. General Prohibitions No
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