Complete volume with articles 1 to 35 - Cucurbit Breeding - North ...
Complete volume with articles 1 to 35 - Cucurbit Breeding - North ... Complete volume with articles 1 to 35 - Cucurbit Breeding - North ...
Figure 1. Emergence of multiple shoots (shoot cluster) from the shoot tip explants of 7 day old seedlings of ‘Poinsett 76’ cucumber. Figure 2. Elongated shoot excised from the shoot cluster. Cucurbit Genetics Cooperative Report 24: 8-12 (2001) 11
concentration of NAA (0.1 mg/l) with optimal concentrations of BAP (1.0 mg/l) was found essential in the induction of adventitious buds and subsequent multiple shoots from the shoot tip explants. At least three subcultures were required to produce the maximum number of shoots per explant. No phenotypic changes were observed in control plants and plants derived from the shoot tip cultures. The system described would be applied for transgenic recovery and in breeding programs. Our results are in agreement with the observation of Plader et al. (4). Literature cited 1. Gambley, R.L., and N.A.Dodd. 1990. An in vitro technique for the production of de novo multiple shoots in cotyledon explants of cucumber (Cucumis sativus L.). Plant Cell Tissue and Organ Culture. 20:177-183. 2. Handley, W.and O.L.Chambliss. 1979. In vitro propagation of Cucumis sativus L HortScience 14:22-23. 3. Murashige, T. and F.Skoog. !962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473- 497. 4. Plader, W., W.Burza, and Z. Rusinowski. 1998. The relationship between the regeneration system and genetic variability in cucumber (Cucumis sativus L.). Euphytica 103:9-15. 5. Schulze, J., C. Balko, B. Zellner, T. Koprek, R. Hansch, A. Nerlich, and R.R.Mendel. 1995. Biolistic transformation of cucumber using embryogenic suspension cultures: Long- term expression of reporter genes. Plant Science. 112: 197-20. Cucurbit Genetics Cooperative Report 24: 8-12 (2001) 12
- Page 1 and 2: Cucurbit Genetics Cooperative Coope
- Page 3 and 4: The Cucurbit Genetics Cooperative (
- Page 5 and 6: this would be a welcome benefit to
- Page 7 and 8: Georgia, Statesboro, GA, “Evaluat
- Page 9 and 10: http://www.ashs.org/ashspress/ wate
- Page 11 and 12: Cucurbit Genetics Cooperative Finan
- Page 13 and 14: Table 1. The composition of culture
- Page 15 and 16: Prezygotic barriers have previously
- Page 17 and 18: Cucumber Inbred Line USDA 6632E Jac
- Page 19 and 20: Multiple Shoot Induction from the S
- Page 21: Table 3. Effects of combinations of
- Page 25 and 26: eplicate. Shoot differentiation and
- Page 27 and 28: Table 2. Shoot induction efficiency
- Page 29 and 30: Figure 1. The viability of Cucumis
- Page 31 and 32: Gynogenesis in a Dihaploid Line of
- Page 33 and 34: Resistant Blister Reaction to Powde
- Page 35 and 36: Table 1. Analysis of variance for p
- Page 37 and 38: Fusarium Wilt Resistance in Eight I
- Page 39 and 40: aised PM seedlings and plants, alth
- Page 41 and 42: Table 1. F2 single factor segregati
- Page 43 and 44: of the loci (e.g., M7675, U13831, A
- Page 45 and 46: Table 1. Percentage of RAPD band pr
- Page 47 and 48: Inodorus Primer Casaba Honeydew Cha
- Page 49 and 50: Frequency of RAPD Polymorphisms in
- Page 51 and 52: BC551 76.6 92.9 38.5 20.5 38.9 42.2
- Page 53 and 54: Powdery Mildew: An Emerging Disease
- Page 55 and 56: Table 1 (continued).z Cultivar Coun
- Page 57 and 58: Disease Severeity Rating Figure 1.
- Page 59 and 60: In the present study C. lanatus ent
- Page 61 and 62: Lakes, NJ) was a 0.5 cc (50 unit) s
- Page 63 and 64: Characterization of a New Male Ster
- Page 65 and 66: Table 1. Progeny test of male steri
- Page 67 and 68: Progeny Diploid Year Season generat
- Page 69 and 70: elatively small during its developm
- Page 71 and 72: content had no significant effect o
Figure 1. Emergence of multiple shoots (shoot cluster) from the shoot tip explants of 7 day old<br />
seedlings of ‘Poinsett 76’ cucumber.<br />
Figure 2. Elongated shoot excised from the shoot cluster.<br />
<strong>Cucurbit</strong> Genetics Cooperative Report 24: 8-12 (2001) 11
Change language