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Figure 1. Globular embryo of C. sativus seven days after in situ pollination. Figure 2. Hybrid embryo of C. sativus x C. melo seven days after in situ pollination. Cucurbit Genetics Cooperative Report 24: 1-5 (2001) 3
Prezygotic barriers have previously been found during interspecific crosses C. melo x C. metuliferus (1), or by crosses of C. melo (2n) x C. melo (4n) (10). However, in our experiments, these kinds of barriers were not observed. Seven days after self-pollination of C. sativus (Fig. 1) and C. melo plants, globular embryos developed. Thereafter, normal development of embryos, seeds and fruits was recorded. The hybrid immature seeds contained globular embryos seven days after pollination (Fig. 2). However, the development of embryos and fruits stopped at this stage. Embryos aborted and immature fruits became yellow in color. This developmental sequence is in agreement with previously published data (8). The abortion of hybrid embryos (postzygotic barrier) could be considered as a main factor in the inability of these Cucumis species to cross fertilize. Differences in fertilization in situ and in vitro. The pollen germination in vitro started 10 min after transfer to the culture medium. This was a shorter time than that demonstrated by germination in situ. The suitability of medium for germination in vitro was demonstrated by the absence of cracked pollen tubes and calloses in tubes. The highest concentration of boric acid and sucrose stimulated pollen germination and pollen tube growth. The tubes length was around 450 µm one hour after germination in both species, and 1350 µm for C. sativus and 1100 µm for C. melo 24 hours after cultivation. At that time, the tubes growth stopped. In contrast to pollination in situ, no taxis of tubes were observed; except for a very small area near the ovules. Penetration of ovules by pollent tubes was noted and globular embryos were detected in both species and their hybrid seven days after cultivation. These results showed that the complete process of sexual reproduction can be accomplished in C. sativus x C. melon matings, and that embryos can be obtained for in vitro cultivation at an early stage of development after fertilization. Literature Cited 1. Beharav, A., Cohen, Y. 1995. Attempts to overcome the barrier of interspecific hybridization between Cucumis melo and C. metuliferus. Israel Journal of Plant Sciences 43, 113-123. 2. Dryanovska, O.A., Ilieva, I.N., 1983. In vitro anther and ovule culture in muskmelon (Cucumis melo L.). Comptes rendus de l´Académie Bulgre des Sciences 36, 8, 16-19. 3. Faris, M. N. and Niemirowicz-Szczytt, K. 1999. Cucumber (Cucumis sativus L.) embryo development in situ after pollination with irradiated pollen. Acta biologica Cracoviensia 41, 111-118. 4. Lebeda, A., 1999. Pseudoperonospora cubensis on Cucumis spp. and Cucurbita spp. - resistance breeding aspects. Acta Hort. 492, 363-370. 5. Lebeda, A., K!ístková, E., Kuba-láková, M. 1996. Interspecific hybridization of Cucumis sativus x Cucumis melo as a potential way to transfer resistance to Pseudoperono-spora cubensis. In: Gómez-Guillamón, M.L., Soria, C., Cuartero, J., Torés, J.A., Fernández-Munoz, R. (eds.): Cucurbits Towards 2000. Proceedings of the VIth Eucarpia Meeting on Cucurbit Genetics and Breeding, May 28-30, 1996, Malaga (Spain), 31-37. 6. Lebeda, A., Kubaláková, M., K!ístková, E., Dole%al, K., Navrátilová, B., Dole%el, J., Lysák, M. 1999. Morphological and physio-logical characteristics of plants issued from an interspecific hybridization of Cucumis sativus x Cucumis melo. Acta Hort. 492, 149-155. 7. Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 474- 497. 8. Niemirowicz-Szczytt, K., Wyszogrod-zka, A., 1976. Embryo culture and in vitro pollination of excised ovules in the family Cucurbitaceae. In: Novák, F.J. (ed.), 1976. Use of tissue cultures in plant breeding. Proc. Int. Symp. Olomouc, 6.-11. Sept., 571-576. 9. Rodkiewicz, B., et al. 1996. Embriologia Angiospermae rozwojo-wa i eksperymentalna. Wydawnictwo UMCS, Lublin, Poland, p. 251- 256. 10. Susín, I., Álvarez, M. J. 1997. Fertility and pollen tube growth in polyploid melons (Cucumis melo L.). Euphytica 93, 369-373. Cucurbit Genetics Cooperative Report 24: 1-5 (2001) 4
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Page 11 and 12: Cucurbit Genetics Cooperative Finan
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Page 17 and 18: Cucumber Inbred Line USDA 6632E Jac
Page 19 and 20: Multiple Shoot Induction from the S
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Page 23 and 24: concentration of NAA (0.1 mg/l) wit
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Page 27 and 28: Table 2. Shoot induction efficiency
Page 29 and 30: Figure 1. The viability of Cucumis
Page 31 and 32: Gynogenesis in a Dihaploid Line of
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Page 37 and 38: Fusarium Wilt Resistance in Eight I
Page 39 and 40: aised PM seedlings and plants, alth
Page 41 and 42: Table 1. F2 single factor segregati
Page 43 and 44: of the loci (e.g., M7675, U13831, A
Page 45 and 46: Table 1. Percentage of RAPD band pr
Page 47 and 48: Inodorus Primer Casaba Honeydew Cha
Page 49 and 50: Frequency of RAPD Polymorphisms in
Page 51 and 52: BC551 76.6 92.9 38.5 20.5 38.9 42.2
Page 53 and 54: Powdery Mildew: An Emerging Disease
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Page 57 and 58: Disease Severeity Rating Figure 1.
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Table 1. Progeny test of male steri
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Progeny Diploid Year Season generat
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content had no significant effect o
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Stars-N-Stripes 26.3 12.0 35 8.5 5.
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11. Mozumder, B.K.G, N.E. Caroselli
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Table 1. Vine length of watermelon
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Survey of Watermelon Trialing Metho
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assigned to adjacent plots (Table 2
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Variation among tropical pumpkin cu
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Table 1. Aggressiveness of powdery
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A large variability of aggressivene
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Table 1: Observed reaction of melon
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Same Gene for Bush Growth Habit in
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Relationship between Fruit Shape an
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Table 3. Seed yield per fruit and s
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The four cultivar-groups differed m
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Lee et al. (17) developed a RAPD ma
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19. Lelley, T and S. Henglmüller,
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Those primers that had strong bands
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Genetic Variability in Pumpkin (Cuc
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those obtained by the ANOVA and the
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Agarose discs were prepared as desc
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Table 4. Effect of different densit
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Isolation and Callus Production fro
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Table 1. Components used for cultur
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6. Jia, Shi-rong, You-ying Fu and Y
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the preceding one or two days (depe
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Gene List 2001 for Cucumber Jiahua
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Ar - Anthracnose resistance. One of
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de I determinate habit. Short vine
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gi - ginkgo. Leaves reduced and dis
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Mdh-4 Mdh- 3 Malate dehydrogenase-4
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h from 'Gy 2 cp cp', 'Spartan Salad
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wf - White flesh. Intense white fle
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M16056 Cotyledon cDNA Encoding ribu
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(for CsPK2.2) AB001590 Hypocotyl RN
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Caruth, T. F. 1975. A genetic study
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John, C. A. and J. D. Wilson. 1952.
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Ohkawa, J., A. Shinmyo, M. Kanchana
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Shimizu, S., K. Kanazawa, and A. Ka
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Youngner, V. B. 1952. A study of th
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Cucurbit Genetics Cooperative 2001-
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Denlinger, Phil Mt. Olive Pickle Co
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Keita, Sugiyama Kurume Branch, Natl
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germplasm evaluation and conservati
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Sarfatti, Matti Hazera Ltd., Resear
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Alabama Fenny Dane Arkansas Ted Mor
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Covenant and By-Laws of the Cucurbi
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ARTICLE IX. General Prohibitions No
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