Biomedical Engineering – From Theory to Applications

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230 Biomedical Engineering – From Theory to Applications Polymer-coated CdSe/ZnS core shell quantum dots were first conjugated to diaminomodified PEG molecules and then to GSH through amide bond formation. The resulting bioconjugated were extensively characterized to confirm the presence of the surface functionalizations (Tortiglione et al., 2007). Both PEG-QDs and PEG-GSH-QDs were supplied to living polyps at different concentrations and then observed by fluorescence microscopy. A biological response consisting in mouth opening and QD entry into the gastric cavity was elicited by GSH-QDs. The elicitation of this behaviour, although slightly different from the classical feeding response (consisting of tentacle writhing and mouth opening) and occurring in a small percentage of animals (15%), was specific for GSH coated QDs, and indicated the bioactivity of the new GSH abduct. Fluorescent QD targeted cells were found within the inner endodermal cells, which internalized the QD upon mouth opening (see Figure 4) (Tortiglione et al., 2007). Fig. 4. In vivo fluorescence imaging of Hydra polyps treated with 300 nM GSH-QDs (emission max: 610 nm). a) Bright field image of Hydra treated with GSH-QDs showing animal basic structure. The foot is on the lower part of the panel, while a crown of tentacles surrounds the mouth. b) Image taken 24 h after treatment: an intense fluorescence is distributed all along the gastric region. c) Cellular localization of QDs in Hydra cross sections. The whole Hydra was treated with 300 nM GSH-QDs for 24 h, fixed in 4% paraformaldehyde, and included for cryosectioning. Images were collected using an inverted microscope (Axiovert, 100, Zeiss) equipped with fluorescence filter sets (BP450-490/FT510/LP515). Endodermal cells(en) are separated from ectodermal cells (ec) by an extracellular matrix, the mesoglea (m), indicated by the arrow. Red fluorescence corresponds to GSH-QDs located specifically into endodermal cells. Scale bars: 500 m in a, b; 200 m in c The fluorescence pattern and intensity lasted unaltered until the animals were fed again, after which the signal started to fade slowly and was diluted throughout the emerging buds (Figure 5).
An Ancient Model Organism to Test In Vivo Novel Functional Nanocrystals Fig. 5. Tracking QD fluorescence under normal feeding regime 231
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BIOMEDICAL ENGINEERING - FROM THEOR
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free online editions of InTech Book
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VI Contents Chapter 9 Targeted Magn
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X Preface stand-alone and readers c
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6 Biomedical search engine Scientif
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12 Bireme http://regional.bv salud.
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110 Method (Detection) Analyte Matr
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120 6. Conclusion Biomedical Engine
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150 5. Conclusions Biomedical Engin
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162 1 st phase : half year 4 2 nd p
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172 12. Maturing projects’ story
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454 In this case, the eigenvalues a
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456 where Biomedical Engineering -
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472 Nb b b l l1 Biomedical Engineer
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478 Load F (μN) Parallel-oriented
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Biomedical Engineering – From Theory to Applications

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