Biomedical Engineering – From Theory to Applications

Column Coupling Electrophoresis in Biomedical Analysis
Fig. 20. ITP-GE (B) versus GE (A) mode of SDS-protein complexes analysis in the sieving
matrix of 10% dextran on microchip. Peak identification: 1, carbonic anhydrase (124
g/mL); 2, ovalbumin (20 g/mL); 3, BSA (50 g/mL); 4, conalbumin (60 g/mL).
Reprinted from ref. (Huang et al., 2005), with permission.
SPE-MCE. The study involved trypsin digestion, affinity extraction of histidinecontaining
peptides, and reversed-phase capillary electrochromatography of the selected
peptides in a single polydimethylsiloxane chip was described by Slentz et al. (Slentz et al.,
2003). Copper (II)-immobilized metal affinity chromatography 5m-particles have been
introduced into the chip. Frits have been fabricated in order to maintain the beads, with
collocated monolithic support structures (COMOSS). They were able to trap particulate
contaminants ranging down to 2m in size. Fig. 21 presents the on-chip separation of
fluorescein isothiocyanate-labeled bovine serum albumin digest (A) before and (B) after
affinity extraction.
Fig. 21. On-chip separation of fluorescein isothiocyanate-labeled bovine serum albumin
digest (A) before and (B) after affinity extraction. Reprinted from ref. (Slentz et al., 2003),
with permission.
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