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Biomedical Engineering – From Theory to Applications

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Column Coupling Electrophoresis in <strong>Biomedical</strong> Analysis<br />

Method (Detection) Analyte Matrix<br />

CZE-MEKC<br />

(UV)<br />

Microdialysis-CZE<br />

(LIF-derivatization)<br />

SPE-CE<br />

(UV)<br />

SPE-CE<br />

(UV)<br />

Tryptic digest of bovine<br />

serum albumin<br />

Glutathione and cystine<br />

Tryptic peptides<br />

SEC-SPE-CE (DAD) Peptides<br />

MLC-CZE<br />

(UV)<br />

Chips<br />

ITP-ZE<br />

(CON)<br />

ITP-ZE<br />

(CON)<br />

ITP-GE<br />

(LIF)<br />

ITP-ZE<br />

(LIF)<br />

ITP-ZE<br />

(LIF)<br />

Extract of<br />

proteins<br />

Rat caudate<br />

nucleus<br />

(in vivo)<br />

Extract of<br />

proteins<br />

Type of<br />

application<br />

Model<br />

<strong>Biomedical</strong><br />

Model<br />

Cefoperazone and ceftiofur Plasma (spiked) Model<br />

Cerebrospinal<br />

fluid (spiked)<br />

Terbutalin (enantiomers) Plasma Model<br />

References<br />

Sahlin,<br />

2007<br />

111<br />

Lada & Kennedy,<br />

1997<br />

Bonneil &<br />

Waldron, 2000<br />

Puig et al.,<br />

2007<br />

Model Tempels et al., 2006<br />

Pálmarsdóttir &<br />

Edholm, 1995<br />

Valproate Serum Ölvecká et al., 2003<br />

Proteins Aqueous Model Ölvecká et al., 2004<br />

Sodium dodecylsulfate<br />

proteins<br />

Aqueous Model<br />

blockers Urine <strong>Biomedical</strong><br />

Fluorescently labeled<br />

ACLARA eTag reporter<br />

molecules<br />

Cell lysate<br />

(spiked)<br />

Model<br />

Huang et al.,<br />

2005<br />

Kriikku et al.,<br />

2004<br />

Wainright et al.,<br />

2002<br />

ZE-ZE Tryptic digest of proteins Cong et al., 2008<br />

ZE-ZE<br />

(LIF)<br />

Membrane filtration-<br />

ZE<br />

(LIF)<br />

SPE-ZE<br />

(LIF)<br />

Gemifloxacin enantiomers<br />

Reduced glutathione<br />

Peptides<br />

Urinary solution<br />

(spiked)<br />

Human plasma<br />

and red blood<br />

cells<br />

Extract of<br />

proteins<br />

Model<br />

Cho et al.,<br />

2004<br />

<strong>Biomedical</strong> Long et al., 2006<br />

Model Slentz et al., 2003<br />

CON= conductivity detection, UV=spectral UltraViolet detection, DAD=diode array detection, LIF=laser<br />

fluorescence detection, MS=mass spectrometry, ITP=capilaary isotachophoresis, CZE=capillary zone<br />

electrophoresis, CE=capillary electrophoresis, ZE=zone electrophoresis, CEC=capillary<br />

electrochroma<strong>to</strong>graphy, MEKC=micellar eelctrokinetic chroma<strong>to</strong>graphy, CIEF=capillary isoelectric<br />

focusing, CGE=capillary gel electrophoresis, GE=gel eelctrophoresis, SEC=size exclusion chroma<strong>to</strong>graphy,<br />

CLC=column liquid chroma<strong>to</strong>graphy, SPE=solid phase extraction, EDTA=ethylendiaminotetraacetic acid.<br />

Table 2. <strong>Applications</strong> of column coupling electrophoretic methods

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