Biomedical Engineering – From Theory to Applications

More documents

Recommendations

Info

108 Method (Detection) CIEF-tITP-CZE (UV) LOD (LOQ) Linearity (r) Biomedical Engineering – From Theory to Applications Precision a (RSD, %) - - 5-10 - 3.5 (218nm) 0.9996 - CZE-CZE (DAD) 0.4 (280nm) 0.9998 CZE-MEKC (UV) - - Microdialysis- CZE (LIF) 0.02 and 0.04 SPE-CE (UV) - - SPE-CE (UV) SEC-SPE-CE (DAD) 100 ng/mL >0.9998 0.9828 - 0.9879 100 ng/mL >0.9950 Recovery (%), (matrix) - 93 – 98 (urine) 1.7 - 4.0 (migration times) 2.5 - 4.9 (peak heights) - ~100 (caudate nucleus) 0.5 - 5.2 (migration - times) 57 – 90 8.1 - 11.9 (plasma) 10 (peak heights) MLC-CZE (UV) - 0.9998 0.7 - 3.5 - Chips 65 (cerebrospinal fluid) ITP-ZE (CON) 90-94 (serum) ITP-ZE (CON) (LOQ 1.10 ng/mL) 3-5.10 3 0.9542 - 0.9999 6.7 - 11.8 - ITP-GE (LIF) - 0.9992 - - ITP-ZE (LIF) 4.10 -7 - - - Membrane filtration-ZE (LIF) 14.6 (plasma) and 8.9 (cells) Remarks References Mohan & Lee, 2002 Danková et al., 2003 Sahlin, 2007 Lada & Kennedy, 1997 Bonneil & Waldron, 2000 Puig et al., 2007 Tempels et al., 2006 Pálmarsdótti r & Edholm, 1995 Ölvecká et al., 2003 Ölvecká et al., 2004 Huang et al., 2005 Wainright et al., 2002 Long et al., 2006 a RSD of peak areas LOD=limit of detection, LOQ=limit of quantification, r=correlation coefficient, RSD=relative standard deviation, CON= conductivity detection, UV=spectral UltraViolet detection, DAD=diode array detection, LIF=laser fluorescence detection, MS=mass spectrometry, ITP=capillary isotachophoresis, CZE=capillary zone electrophoresis, CE=capillary electrophoresis, ZE=zone electrophoresis, CEC=capillary electrochromatography, MEKC=micellar eelctrokinetic chromatography, CIEF=capillary isoelectric focusing, CGE=capillary gel electrophoresis, GE=gel eelctrophoresis, SEC=size exclusion chromatography, CLC=column liquid chromatography, SPE=solid phase extraction. Table 1. Performance parameters of column coupling electrophoretic methods
Column Coupling Electrophoresis in Biomedical Analysis 5. Applications of column coupling electrophoresis The column coupled methods, described in previous sections (3 and 4) concerning the theory (principles, experimantal arrangement, benefits, limitations) and performance parameters (LOD, precision, recovery/accuracy, etc.), have been used in many applications. Capillary (CE) and microchip (MCE) formats were taken into consideration. CE and MCE determinations of various biologically active compounds in model (spiked samples) as well as real biological matrices, employing an on-line coupling of the electrophoretic methods with other electrophoretic or non electrophoretic methods, are listed in Table 2. In this table we tried to emphasize briefly the most important features and characteristics of the applications of column coupling electrophoresis in capillary as well as in chip arrangement. This table is further supported by the text where other relevant parameters and explanations are included for the real bioapplications and proteinic analysis. Method (Detection) Analyte Matrix Capillary arrangement ITP-CZE (CON) ITP-CZE (UV) ITP-CZE (UV) ITP-CZE (UV) ITP-CZE (DAD-spectral informations) ITP-CZE (DAD-spectral informations) ITP-CZE (DAD-spectral informations) ITP-CZE (DAD-spectral informations) ITP-CZE (DAD-spectral informations) ITP-CZE (UV) Valproic acid Serum Lamotrigine Serum 5-methyltetrahydrofolate Sulphanilate, 3,5-dinitrosalicylate Orotic acid Urine Amlodipine Urine Amlodipine (enantiomers) Urine Celiprolol Urine Pheniramine (enantiomers) Urine Pheniramine, dimethindene, dioxopromethazine (enantiomers) Blood, serum and urine Type of application Biomedical (Theurapeutic drug monitoring) Biomedical Urine (spiked) Model Urine Biomedical (biomarker analysis) References Budáková et al., 2007 Budáková et al., 2009 109 Pantůčková et al., 2010 Kaniansky et al., 1993 Danková et al., 2001 Biomedical Mikuš et al., (pharmakokine 2009 tic study) Biomedical Mikuš et al., (pharmakokine 2008b tic study) Biomedical Mikuš et al., (pharmakokine 2008a tic study) Biomedical (metabolic study) Biomedical (metabolic study) Marák et al., 2007 Mikuš et al., 2006
Page 1 and 2:
BIOMEDICAL ENGINEERING - FROM THEOR
Page 3:
free online editions of InTech Book
Page 6:
VI Contents Chapter 9 Targeted Magn
Page 10:
X Preface stand-alone and readers c
Page 14 and 15:
2 Biomedical Engineering - From The
Page 16 and 17:
4 Fig. 2. Process for retrieval inf
Page 18 and 19:
6 Biomedical search engine Scientif
Page 20 and 21:
8 Biomedical Engineering - From The
Page 22 and 23:
10 Biomedical Engineering - From Th
Page 24 and 25:
12 Bireme http://regional.bv salud.
Page 26 and 27:
14 Semantic Networks analysis Biome
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71: 58 Biomedical Engineering - From Th
Page 112 and 113: 100 Biomedical Engineering - From T
Page 122 and 123: 110 Method (Detection) Analyte Matr
Page 132 and 133: 120 6. Conclusion Biomedical Engine
Page 162 and 163: 150 5. Conclusions Biomedical Engin
Page 170 and 171:
158 Biomedical Engineering - From T
Page 172 and 173:
Page 174 and 175:
162 1 st phase : half year 4 2 nd p
Page 176 and 177:
Page 178 and 179:
166 7. Innovative active training B
Page 180 and 181:
Page 182 and 183:
Page 184 and 185:
172 12. Maturing projects’ story
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
180 16. Acknowledgments Biomedical
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 202 and 203:
190 R* M M M M MR*M O O O O M O R*
Page 204 and 205:
Page 206 and 207:
Page 208 and 209:
196 Fig. 9. Chemical structure of 5
Page 210 and 211:
198 Relative Intensity (AU) Biomedi
Page 212 and 213:
Page 214 and 215:
Page 216 and 217:
204 2. Preparation of IO nanopartic
Page 218 and 219:
Page 220 and 221:
Page 222 and 223:
Page 224 and 225:
Page 226 and 227:
Page 228 and 229:
Page 230 and 231:
Page 232 and 233:
Page 234 and 235:
Page 236 and 237:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
Page 244 and 245:
Page 246 and 247:
Page 248 and 249:
Page 250 and 251:
Page 252 and 253:
Page 254 and 255:
Page 256 and 257:
Page 258 and 259:
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
256 3. Deposition techniques Biomed
Page 270 and 271:
Page 272 and 273:
Page 274 and 275:
Page 276 and 277:
Page 278 and 279:
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
Page 288 and 289:
Page 290 and 291:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304 and 305:
Page 306 and 307:
Page 308 and 309:
Page 310 and 311:
Page 312 and 313:
300 2. Nanoparticles in biomedical
Page 314 and 315:
Page 316 and 317:
Page 318 and 319:
Page 320 and 321:
Page 322 and 323:
Page 324 and 325:
Page 326 and 327:
Page 328 and 329:
Page 330 and 331:
Page 332 and 333:
Page 334 and 335:
Page 336 and 337:
Page 338 and 339:
Page 340 and 341:
Page 342 and 343:
Page 344 and 345:
Page 346 and 347:
Page 348 and 349:
Page 350 and 351:
Page 352 and 353:
Page 354 and 355:
Page 356 and 357:
Page 358 and 359:
Page 360 and 361:
Page 362 and 363:
Page 364 and 365:
Page 366 and 367:
Page 368 and 369:
Page 370 and 371:
Page 372 and 373:
Page 374 and 375:
Page 376 and 377:
Page 378 and 379:
Page 380 and 381:
Page 382 and 383:
Page 384 and 385:
Page 386 and 387:
Page 388 and 389:
Page 390 and 391:
Page 392 and 393:
Page 394 and 395:
Page 396 and 397:
Page 398 and 399:
Page 400 and 401:
Page 402 and 403:
Page 404 and 405:
Page 406 and 407:
Page 408 and 409:
Page 410 and 411:
Page 412 and 413:
Page 414 and 415:
Page 416 and 417:
Page 418 and 419:
Page 420 and 421:
Page 422 and 423:
Page 424 and 425:
Page 426 and 427:
Page 428 and 429:
Page 430 and 431:
Page 432 and 433:
Page 434 and 435:
Page 436 and 437:
Page 438 and 439:
Page 440 and 441:
Page 442 and 443:
Page 444 and 445:
Page 446 and 447:
434 3. Three dimensional virtual mo
Page 448 and 449:
Page 450 and 451:
Page 452 and 453:
Page 454 and 455:
Page 456 and 457:
Page 458 and 459:
Page 460 and 461:
Page 462 and 463:
Page 464 and 465:
Page 466 and 467:
454 In this case, the eigenvalues a
Page 468 and 469:
456 where Biomedical Engineering -
Page 470 and 471:
Page 472 and 473:
Page 474 and 475:
Page 476 and 477:
Page 478 and 479:
Page 480 and 481:
Page 482 and 483:
Page 484 and 485:
472 Nb b b l l1 Biomedical Engineer
Page 486 and 487:
Page 488 and 489:
Page 490 and 491:
478 Load F (μN) Parallel-oriented
Page 492 and 493:
Page 494 and 495:
Page 496 and 497:
Page 498:
show all

Biomedical Engineering – From Theory to Applications

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?