Biomedical Engineering – From Theory to Applications

108
Method
(Detection)
CIEF-tITP-CZE
(UV)
LOD
(LOQ)
Linearity
(r)
Biomedical Engineering – From Theory to Applications
Precision a
(RSD, %)
- - 5-10 -
3.5 (218nm) 0.9996 -
CZE-CZE (DAD)
0.4 (280nm) 0.9998
CZE-MEKC (UV) - -
Microdialysis-
CZE (LIF)
0.02 and
0.04
SPE-CE (UV) - -
SPE-CE (UV)
SEC-SPE-CE
(DAD)
100 ng/mL
>0.9998
0.9828 -
0.9879
100 ng/mL >0.9950
Recovery (%),
(matrix)
- 93 – 98 (urine)
1.7 - 4.0
(migration
times)
2.5 - 4.9
(peak
heights)
-
~100 (caudate
nucleus)
0.5 - 5.2
(migration -
times)
57 – 90
8.1 - 11.9
(plasma)
10
(peak
heights)
MLC-CZE (UV) - 0.9998 0.7 - 3.5 -
Chips
65
(cerebrospinal
fluid)
ITP-ZE (CON) 90-94 (serum)
ITP-ZE (CON)
(LOQ
1.10
ng/mL)
3-5.10 3
0.9542 -
0.9999
6.7 - 11.8 -
ITP-GE (LIF) - 0.9992 - -
ITP-ZE (LIF) 4.10 -7 - - -
Membrane
filtration-ZE
(LIF)
14.6
(plasma)
and 8.9
(cells)
Remarks References
Mohan &
Lee, 2002
Danková et
al., 2003
Sahlin,
2007
Lada &
Kennedy,
1997
Bonneil &
Waldron,
2000
Puig et al.,
2007
Tempels et
al., 2006
Pálmarsdótti
r & Edholm,
1995
Ölvecká et
al., 2003
Ölvecká et
al., 2004
Huang et al.,
2005
Wainright et
al., 2002
Long et al.,
2006
a RSD of peak areas
LOD=limit of detection, LOQ=limit of quantification, r=correlation coefficient, RSD=relative standard
deviation, CON= conductivity detection, UV=spectral UltraViolet detection, DAD=diode array
detection, LIF=laser fluorescence detection, MS=mass spectrometry, ITP=capillary isotachophoresis,
CZE=capillary zone electrophoresis, CE=capillary electrophoresis, ZE=zone electrophoresis,
CEC=capillary electrochromatography, MEKC=micellar eelctrokinetic chromatography, CIEF=capillary
isoelectric focusing, CGE=capillary gel electrophoresis, GE=gel eelctrophoresis, SEC=size exclusion
chromatography, CLC=column liquid chromatography, SPE=solid phase extraction.
Table 1. Performance parameters of column coupling electrophoretic methods