GROWING GOURMET - Anto2ni.it

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Viii GROWING GOURMET AND MEDICINAL MUSHROOMS Soybean Waste 55 Supplements 55 Structure of the Habitat 56 7. Biological Efficiency: An Expression of Yield 57 8. Home-madevs.CommercialSpawn 61 9. 'I'he 1\'Iiishroorn Life (ycle 65 10. The Six Vectors ofContaniination . . . . . 75 11. MindandMethodsforMushroomCulture 83 Overview of, Techniques for Cultivating Mushrooms 85 12. CulturingMushroomMyceliumonAgarMedia ......... 89 Preparing Nutrified Agar Media 89 Malt Extract, Yeast Agar 90 Potato, Dextrose, Yeast Agar 90 Oatmeal, Malt, Yeast Enriched Agar 90 DogFoodAgar 90 Corn Meal, Yeast, Glucose Agar 90 Nitrogen & Carbohydrate Supplements 91 End-Substrate Supplements 92 Pouring Agar Media 92 Starting a Mushroom Strain by Cloning 93 Cloning Wild Specimens vs. Cloning Cultivated Mushrooms 96 How to Collect Spores 96 Germinating Spores 100 Purifying a Culture 101 13. The Stock Culture Library: A Genetic Bank of Mushroom Strains..... 103 Preserving the Culture Library 104 The Stamets "P" Value System for Age Determination of a Strain 106 Iconic Types of Mushroom Mycelium 108 The Event of Volunteer Primordia on Nutrified Agar Media 115 14. EvaluatingaMushroomStrain . .................. 117 28 Features for Evaluating and Selecting a Mushroom Strain 118 15. Generating Grain Spa'svn . 127 Formulas for Creating Grain Spawn 129 Grain Formulas for Spawn Production 130 First Generation Grain-Spawn Masters 133 Steps for Generating Grain Spawn Masters 134 Second and Third Generation Grain Spawn 135 PDF compression, OCR, web-optimization with CVISION's PdfCompressor
GROWING GOURMET AND MEDICINAL MUSHROOMS IX Steps for Creating Second and Third Generation Grain Spawn 136 Autoclavable Spawn Bags 139 Liquid Inoculation Techniques 142 Spore Mass Inoculation 142 Liquid Inoculation Techniques: Mycelial Fragmentation and Fermentation 146 Pelletized (Granular) Spawn 151 Matching the Spawn with the Substrate: Critical Choices on the Mycelial Path 152 Spawn Storage 153 16. treating Sasvdtist Spawn 155 Step-by-Step Instructions for Inoculating Sawdust 156 17. Growing Gourmet Mushrooms on Enriched Sawdust 161 The Supplemented Sawdust "Fruiting" Formula: Creating the Production Block 162 Testing For Moisture Content 164 Choosing a Sterilizer, a.k.a the Retort or Autoclave 165 Sterilization of Supplemented Substrates 167 Post-Autoclaving 170 Unloading the Autoclave 170 Atmospheric Steam Sterilization of Sawdust Substrates 171 Inoculation of Supplemented Sawdust: Creating the Production Block 173 Incubation of the Production Blocks 176 Achieving Full Colonization on Supplemented Sawdust 177 Handling the Bags Post Full Colonization 179 18. Cultivating Gourmet Mushrooms on Agricultural Waste Products .... 181 Alternative Fruiting Formulas 182 Heat Treating the Bulk Substrate 183 Alternative Methods for Rendering Straw & other Bulk Substrates for Mushroom Cultivation 189 19. Cropping Containers 191 Tray Culture 192 Vertical Wall Culture 195 Slanted Wall or "A" Frame Culture 196 Bag Culture 196 Column Culture 198 Bottle Culture 204 20. Casing: A Topsoil Promoting Mushroom Formation . .... 209 21. Growth Parameters for Gourmet and Medicinal Mushroom Species... 211 Spawn Run: Colonizing the Substrate 212 Primordia Formation: The Initiation Strategy 213 PDF compression, OCR, web-optimization with CVISION's PdfCompressor
Page 1 and 2: GROWING GOURMET and MEDICINAL MUSHR
Page 3 and 4: This book should help advance the c
Page 5 and 6: GROWING GOURMET AND MEDICINAL MUSHR
Page 7: GROWING GOURMET AND MEDICINAL MUSHR
Page 17 and 18: CHAPTER 1 Mushrooms, Civilization &
Page 19 and 20: Immortality (1976) where he postula
Page 21 and 22: CHAPTER 2 The Role of Mushrooms in
Page 23 and 24: tive species and planted in plots s
Page 25 and 26: ducing organ: the mushroom. Further
Page 27 and 28: fungi first to capture a twig, a bl
Page 29 and 30: The Global Environmental Shift and
Page 31 and 32: nm peroxidases and cellulases which
Page 33 and 34: CHAPTER 3 S electing a Candidate fo
Page 35 and 36: SELECTING A CANDIDATE FOR CULTIVATI
Page 37 and 38: N atural CHAPTER 4 Natural Culture:
Page 39 and 40: NATURAL CULTURE: CREATING MYCOLOGIC
Page 57 and 58: CHAPTER 5 The Stametsian Model: Per
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PERMACULTURE WITH A MYCOLOGICAL TWI
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PERMACULTURE WITH A MYCOLOGICAL TWI
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CHAPTER 6 Materials for Formulating
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MATERIALS FOR FORMULATING A FRUITIN
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CHAPTER 7 Biological Efficiency: An
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BIOLOGICAL EFFICIENCY: AN EXPRESSIO
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CHAPTER8 Home-made vs. Commercial S
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HOME-MADE VERSUS COMMERCIAL SPAWN 6
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CHAPTER 9 The Mushroom Life Cycle W
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Figure 44. Scanning electron microg
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scape, the inycosphere, will give r
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Figure 51. A fully mature basidium.
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posites. The method of spore ejecti
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C ultivating CHAPTER 10 The Six Vec
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floor. After a minute or two, the c
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The best one can hope is that conta
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Figure 60. Storing petri mobile cre
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S terile CHAPTER 11 Mind and Method
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MIND AND METHODS FOR MUSHROOM CULTI
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MIND AND METHODS FOR MUSHROOM CULTI
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CHAPTER 12 Culturing Mushroom Mycel
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CULTURING MUSHROOM MYCELIUM ON AGAR
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E very CHAPTER 13 The Stock Culture
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If one has ten or more replicates,
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eads: FVITC P2 11/16/92 C # 0825905
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Figure 87. Rhizomorphic mycelium di
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exemplified by Laetiporus suiphureu
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molds belonging to Penicillium, Asp
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duce odors which humans can recogni
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CHAPTER 14 Evaluating a Mushroom St
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Figure 96. Healthy mushroom myceliu
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EVALUATING sustained. Without secon
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14. Duration between 1st, 2nd and 3
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substantially in flavor. The cultiv
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G rain CHAPTER 15 Generating Grain
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Figure 101. One memod for preparing
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is added proportionately. Whereas t
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grams. 4 cups is approximately a li
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t .,..,..,,,.. ,'"'•• Figure 10
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Figure 111. Grain inoculated with m
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Figure 113. Gallon jars of 3rd gene
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ag resembles those still widely in
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Figure 116. Scanning electron nucro
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F— GENERATING GRAIN SPAWN 145 Fig
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Figure 120. Actively growing myceli
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Figure 122. Inoculating mycelium in
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the norm. The jars normally grow ou
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Figure 124. (Jyster mushrooms out"
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S awdust CHAPTER 16 Creating Sawdus
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Figure 125. Sawdust spawn of Reishi
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Figure 129. Dispersing the spawn th
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CHAPTER 17 Growing Gourmet Mushroom
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GROWING GOURMET MUSHROOMS ON ENRICH
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C H A P T E R 1 8 Cultivating Gourm
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ice, oat and sorghum straws are the
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Figure 146. A brick keeps the straw
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Figure 149. Newly constructed, scre
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spawn is gravity fed or hand broadc
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C hoosing CHAPTER 19 Cropping Conta
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onments typically ensue until the p
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Figure 158. The Phoenix Oyster, Ple
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Figure 162. The hinged wall-frame
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Figure 167. Bouquets of Golden Oyst
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Figure 171. By slamming the culumn
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latedsubstrate.An 8-ft. longcolumn,
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came a template for the cultivation
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CROPPING CONTAINERS 207 Figures 182
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CHAPTER 20 Casing: A Topsoil Promot
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CHAPTER 21 Growth Parameters for Go
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Figure 188. The Button mushroom (Ag
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of the surface, the region supporti
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other gourmet and medicinal mushroo
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INDOOR GROWTH PARAMETERS The Gilled
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Figure 193.20 days after inoculatio
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Growth Parameters Spawn Run: Incuba
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Harvest Hints: Since this mushroom
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The Enoki Mushroom Fkxmmulina velut
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GROWTH PARAMETERS 233 aspen, willow
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GROWTH PARAMETERS 235 short period
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Introduction: Before the publicatio
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GROWTH PARAMETERS 24f doors, this m
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Figure 216. Inoculated logs of Kuri
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GROWTH PARAMETERS 247 spores of spe
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In Japan, H. tessulatus is marketed
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I Figure 224. Commercial cultivatio
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GROWTH PARAMETERS 255 Figures 226 a
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The Shiitake Mushroom of the Genus
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GROWTH PARAMETERS 261 Figure 231 &
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Figure 235. After soaking, logs can
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GROWTH PARAMETERS 265 Figure 240. A
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GROWTH PARAMETERS 269 This species
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Figure 249. The "Donko" form of Shi
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When I was a impoverished, near-sta
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GROWTH PARAMETERS 275 nated) for 24
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The Nameko Mushroom of the Genus Ph
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GROWTH PARAMETERS 281 Natural Metho
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The Oyster Mushrooms of the Genus P
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Introduction: Few mushrooms are as
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259. Bottle culture of P. cttrtnopl
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Figure 262. A beautiful clustered b
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oom Kit. ri Pertecti's oyster Musn-
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Figure Wild fruiting of P cystidios
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Pleurotus djamor (Fries) Boedjin se
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GROWTH PARAMETERS 299 studies are c
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Medicinal Properties: Not known to
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Figure 275 and 276. P. eryngii frui
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GROWTH PARAMETERS 309 Pleurotus euo
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GROWTH PARAMETERS 313 Pleurotus ost
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Figure 282. A sporeless strain of P
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Suggested Agar Culture Media: MYPA,
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Spawn Run: Temperature: 75° F. (24
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Plate 1. Fruiting culture of Agrocy
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Plate 7. Flammulina velutipes, the
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Plates 14. Shiitake mushrooms, Lent
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Plate 22. Pleurotus eryngii, the Ki
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Plate 44. Two strains of Ganoderma
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Plate 51. Succulent bouquet of .Mai
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Pleurotuspulmonarius (Fries) Quelet
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Figure 287. F. pulmonarius fruiting
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GROWTH PARAMETERS 327 The Caramel C
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GROWTH PARAMETERS 329 Psilocybe whi
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dicinal properties, instead focusin
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The King Stropharia of the Genus St
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Figure 297. Azureus Stamets holding
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GROWTH PARAMETERS 341 room can also
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GROWTH PARAMETERS 343 The Paddy Str
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Figure 303. Soaked straw is thrown
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GROWTH PARAMETERS 349 Figure 312—
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INDOOR GROWTH PARAMETERS T he The P
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GROWTH PARAMETERS 353 tude sickness
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GROWTH PARAMETERS 355 Ganoderma luc
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figure 318. U. lucidum (F'orintek's
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Natural Habitat: An annual mushroom
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GROWTH PARAMETERS 361 Figure 323—
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GROWTH PARAMETERS 363 Figures 327
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GROWTH PARAMETERS 367 Natural Metho
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GROWTH PARAMETERS 369 Comments: A s
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GROWTH PARAMETERS 371 form. Microsc
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Iligure 335. Maitake usually truits
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GROWTH PARAMETERS 377 1992, dried M
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GROWTH PARAMETERS 379 Fruitbody dev
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Figure 341. Sclerotia of North Amer
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GROWTH PARAMETERS 385 placed into a
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INDOOR GROWTH PARAMETERS The Lion's
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GROWTH PARAMETERS 389 Description:
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GROWTH PARAMETERS 393 Figure 349. W
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INDOOR GROWTH PARAMETERS The Wood E
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GROWTH PARAMETERS 397 A. polytricha
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INDOOR GROWTH PARAMETERS M orels Th
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GROWTH PARAMETERS 403 Figure 357. T
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GROWTH PARAMETERS 405 thousands per
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Figure 363. A Morel primordium form
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Common Names: The Black Morel The C
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GROWTH PARAMETERS 413 Figure 372 &
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T e m p e r a U r e (°C) Profile o
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Figure 375. This mycological experi
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Growth Parameters* Spawn Run: Incub
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CHAPTER 22 Maximizing the Substrate
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dergoes another 50% reduction in ma
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CHAPTER 23 Harvesting, Storing, and
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Figure 378. Bouquets of Pleurotus o
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Figure 380. An example of poor pack
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P. a large blower located at one en
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CHAPTER 24 Mushroom Recipes: Enjoyi
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MUSHROOM RECIPES 433 Jack Czarnecki
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MUSHROOM RECIPES 435 Hope & Orson M
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MUSHROOM RECIPES 437 Robert Roselli
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Andrew Weil's Shiitake Teriyaki MUS
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MUSHROOM RECIPES 441 Recommended Mu
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T his CHAPTER 25 Cultivation Proble
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TROUBLESHOOTING 445 small tree frog
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Contaminants localized to point of
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TROUBLESHOOTING 449 PROBLEM CAUSE S
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APPENDIX I Descriptions of Environm
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proof electrical fixtures are essen
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neer systems with mushroom preserva
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APPENDIX II Designing and Building
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DESIGNING AND BUILDING A SPAWN LABO
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APPENDIX III The Growing Room: An E
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MOUTH MUSHROOM-CAVE NEAR PARI& THE
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Many farms employ pass-through curt
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move debris from the feet and enhan
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changes per hour required during th
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After washing, the growing room fee
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APPENDIX IV Resource Directory T he
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Mushroom Book Suppliers Fungi Perfe
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Mushroom Cultivation Seminars & Tra
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RESOURCE DIRECTORY 487 Chibougamau
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RESOURCE DIRECTORY 489 Nutmeg Mycol
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Centre for Land & Biological Resear
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J. B. Swayne Spawn (215) 444-0888 8
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RESOURCE DIRECTORY 495 The Mushroom
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Material APPENDIX V Analysis of Bas
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Material ANALYSIS OF B ASIC MAT ERI
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Material ANALYSIS OF BASI C M ATERI
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Material ANALYSIS OF B ASI C M ATER
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Material ANALYSIS OF BASI C M ATERI
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Material ANALYSIS OF BASI C MATERIA
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Material ANALYSIS OF BASI C MATERIA
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Material ANALYSIS OF BASI C M AT ER
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APPENDIX VI Data Conversion Tables
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1°F. = •5550 Kelvin 1° F = (.55
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DATA CONVERSION TABLES 517 Miscella
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Glossary A agar: a product derived
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droplets of spore bundles on relati
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mycophagist: a person or animal tha
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metabolic heat released as fungi, b
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Bibliography Adachi, K., H. Nanba,
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BIBLIOGRAPHY 529 Chang, S.T. &W.A.
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______ BIBLIOGRAPHY 531 Gormanson,D
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_____1995. BIBLIOGRAPHY 533 Ito, T.
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wheat straw. Mushroom Journal of th
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_____J.A. 1991. Manual on mushmom c
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_____1987b. BIBLIOGRAPHY 539 Royse,
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_____,A. BIBLIOGRAPHY 541 Stijve, T
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543 1993. Boost immunity with medic
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A agaricum 351 agarikon 351 agar me
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ganoderic acids 368 Ganoderma 353,
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N Nuematoloma N. capnoides 237 N. f
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spore germination 100 spore load 24
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Alan Bessette Figures 52, 53 Julie
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