GROWING GOURMET - Anto2ni.it

450T R 0 U B L ES H 00 TIN G
PROBLEM
CAUSE
Incubation at too high a
temperature
SUPPLEMENTED SAWDUST CULTURE
Mycelium fails to grow Bags inoculated too hot
through within two weeks
Insufficient spawning rate
Mycelium grows & then
stops. Often accompanied by
foul odors, slimy fluids.
Yellow, green, or black molds
Inadequate mixing
of spawn through sawdust
Mis-match of mycelium &
wood type
Sawdust too dry
Sawdust over-sterilized
Presence of contaminantsbacteria
or molds
Inadequate sterilization
Sterilization
sufficient but contaminated
during cool-down
Grain or Sawdust
spawn infected
Person inoculating
introduced contaminants
Bags not separated to
allow heat loss during
incubation
Airborne
contamination
Excessive carbon
dioxide levels during
incubation (>25%)
SOLUTION
Lower incubation temperature.
Allow to cool before substrate
is inoculated
Increase spawning rate.
mixing andlor
spawn rate.
Use woods native to mushroom.
Increase moisture.
Reduce sterilization time.
Consult Chapter 10:
The Six Vectors oftion
Contamination.
Increase sterilization time
Filter air during cool-down,
check autoclave
seals, drains, etc.
Use pure spawn.
Adhere to good sterile
technique.
Space bags 2 in. apart,
maintain air temperature
at 75° F. (24° C.).
Use HEPA filters & good
sterile technique.
Increase surface area,
or transpiration rate of filter
media.
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