GROWING GOURMET - Anto2ni.it

448 TROUBLESHOOTING
PROBLEM CAUSE SOLUTION
Mycelium grows but
spottily/incompletely
Grain spawn difficult
to break-up
Grain spawn appears
pure, but contaminates
with mold or bacteria
after inoculation.
Jars crack, bags break
Over-sterilized
Culture not receptive to
media formula
Insufficient distribution
of mycelium through grain
Insufficient inoculation rate
Bacterial contamination
Over-incubation
Excessive water
Use different type of grain
Mycelium endemically
contaminatedco-existing
with other
organism(s)
Over-incubation
Underside of filter
ladened with organic debris,
providing a medium for
contaminants to grow through.
Contamination airborne or
from lab personnel.
Radical fluctuation
in temperature and/or pressure
Inadequate quality
Reduce sterilization time.
Alter media formula.
Shake jars with greater
frequency.
Increase amount of mycelium
placed into each grain jar.
Clean up strain.
Increase sterilization time of
grain.
Use spawn sooner.
Reduce water in formula by
10-20%.
Use rye, wheat or millet
Add gypsum. See page 130.
Return to stock cultures
or clean strain.
Use spawn sooner. Normal for
old spawn to eventually
support other microorganisms
Soak filter discs in bleach
solution in between
spawn runs.
Install micron filters. Observe
good sterile technique.
Reduce temperature &
pressure flux.
Acquire higher quality, heat
tolerant jars & bags.
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