GROWING GOURMET - Anto2ni.it

366 GROWTH PARAMETERS
exponential expansion of mycelial mass. The
simplest method for most cultivators to follow
is similar to the classic spawn expansion schedule
employed by most laboratories. The first
stage is to grow the mycelium on nutrified agar
media in petri dishes. The next is transferring
pure cultures onto sterilized grain, typically in
jars (quart, half gallons, gallons).At75° F. (24°
C.), two to three weeks pass before colonization
is complete. Each of these "Grain
Masters" can inoculate 10 gallon jars each containing
1000-1200 grams of sterilized rye
grain. Once grown out, each gallon jar of
spawn can readily inoculate 10 5 lb. bags of
hardwood sawdust (wet weight, 65-70% moisture).
The resulting sawdust spawn is the last
step before inoculating a substrate capable of
supporting fruitbodies.
At this juncture, the cultivator
has several options. Four are: to grow fruitbodies on sawdust/chips;
to grow fruitbodies on buried logs; to inoculate stumps; or to fruit Ganoderma lucidum on vertically
arranged columns of heat treated sawdust. (This last method is currently under development by the
author.) These four strategies all follow the same expansion schedule from agar-to-grain-to-sawdustto
the production block.
Suggested Agar Culture Media: MEA. OMYA, PDYA and/or DFA. Uninhibited by gentamycin
sulfate (1/15th gram/liter.)
1st Generation Spawn Media: Rye grain, wheat grain, other cereal grains. Fruitbodies do not form
on most grains except milo (a type of sorghum), whereupon fans of growth climb the inside surfaces
of the spawn containers and fruit within. If mature grain spawn is not Figure 331. Brown spores, although released from
the underside, tend to collect on the upper cap surfaces
of G. lucidum.
used directly after colonization,
over-incubation results, making it difficult to disperse the grain kernels upon shaking. In this case, a
transfer tool such as a sterilized spoon, knife, or similar tool is needed to break apart the spawn within
the jar.
I prefer the liquid inoculation method of generating spawn similar to that described in The
Mushroom Cultivator by Stamets & Chilton (1983). Further, the mycelium of this species readily
adapts to submerged fermentation. Submerged fermentation (liquid culture) of Ganoderrna lucidum
mycelium is considered "traditional" in China.
2nd & 3rd Generation Spawn Media: Each unit of primary spawn can be expanded (3 cups
grain in 1/2 gallon jar) into 10 (5-20) units of 5 lbs. sterilized, moist sawdust. Once inoculated,
and incubated at 75 ° F. (24° C.) colonization is complete in 8-12 days. The 10 sawdust spawn
blocks can be expanded into 100-200 3-5 lbs. sawdust/chip bags, which in turn, are colonized in a
similar period of time.
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