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GROWING GOURMET - Anto2ni.it

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176<br />

<strong>GROWING</strong> <strong>GOURMET</strong> MUSHROOMS ON ENRICHED SAWDUST<br />

servation. If the leak is due to a puncture, the<br />

hole is covered w<strong>it</strong>h plastic packaging tape. If<br />

the seal is imperfect, the bag is returned to the<br />

First Assistant for a second try at heat sealing.<br />

Once each bag has been properly sealed and assured<br />

of proper labelling, thorough shaking is<br />

in order. Using a combination of ag<strong>it</strong>ation and<br />

rotation, the sawdust spawn is mixed through<br />

the supplemented sawdust. The sawdust spawn<br />

has a lighter color than the supplemented sawdust<br />

and hence <strong>it</strong> is easy to see when the spawn<br />

has been evenly dispersed.<br />

The Second Assistant gently slams the<br />

sealed, domed bag on a tabletop to close any<br />

open spaces, and increase the dens<strong>it</strong>y of the<br />

mass. (In shaking, the mixture becomes qu<strong>it</strong>e<br />

loose.) The bag is pos<strong>it</strong>ioned on a wire shelf<br />

where daily observations are made for the next<br />

few weeks. The bags are kept at least a fingerbreadth<br />

apart These newly inoculated,<br />

incubating bags must not touch one another<br />

Now that the duties of the three laboratory<br />

personnel are clearly defined, working in concert<br />

becomes the foremost prior<strong>it</strong>y. A wellorganized<br />

laboratory team achieves a furious<br />

pace. Conversation is kept to a minimum. (The<br />

person doing the inoculations can't talk anyhow,<br />

except between sets of inoculations, lest<br />

his breath spread bacteria. Wearing a filter<br />

mask reduces this risk.) However, times arise<br />

when one or more of the three-person production<br />

flows is interrupted. During these<br />

down-times, counter-tops should be vacuumed<br />

and wiped clean w<strong>it</strong>h alcohol. Garbage can be<br />

consolidated. Finally, one's hands are washed<br />

prior to any more inoculation activ<strong>it</strong>y. The pace<br />

during the inoculation process should be both<br />

rhythmic and fast. Inoculations are purposely<br />

interrupted after every 50 or so bags so periodic<br />

cleaning can occur. Depending on the<br />

equipment, design of the facil<strong>it</strong>y, and experi-<br />

ence of the laboratory personnel, better ways of<br />

organizing the labor during inoculations will<br />

naturally evolve. However, this method works<br />

well. Hundreds of bags can be inoculated during<br />

a single shift. Greater efficiency is realized<br />

if two or more sealers are employed simulta-<br />

neously.<br />

After inoculation, each bag can be shaken by<br />

hand. Larger farms place the bags onto a grav<strong>it</strong>y<br />

conveyor leading to a multiple bag,<br />

automatic shaking machine. As the crew's performance<br />

improves w<strong>it</strong>h experience, block<br />

production soars into the thousands per shift. W<strong>it</strong>h<br />

each bag yielding $ 10-50 + U.S., every doubling<br />

of production over a baseline level, realizes proportionally<br />

prof<strong>it</strong>s for the owners.<br />

Once shaken the inoculated bags are placed<br />

on open wire shelves and spaced about 1 inch<br />

apart for incubation. Bags contacting one another<br />

are likely to contaminate w<strong>it</strong>h black pin<br />

or other thermophilic molds.<br />

Incubation of the<br />

Production Blocks<br />

The first two weeks of incubation after inoculation<br />

are the most cr<strong>it</strong>ical. If the<br />

supplemented sawdust is not fully colonized<br />

during that time period, contamination usually<br />

arises soon thereafter. W<strong>it</strong>hin several days of<br />

inoculation, out-gassing of volatile by-products<br />

causes a distinctly noticeable fragrance.<br />

As soon as the laboratory is entered, the atmosphere<br />

imparts a unique "odor signature?' The<br />

smell is generally described as sweet, pleasant,<br />

and refreshing.<br />

The incubation room should be maintained<br />

at 750 F. (24° C.) and have an ambient humid<strong>it</strong>y<br />

between 30-50%. Since the internal temperatures<br />

of the incubating blocks are often 20° F.<br />

higher than ambient air temperatures, keeping<br />

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