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GROWING GOURMET - Anto2ni.it

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Figure 116. Scanning electron nucrograph of spores<br />

in a frenzied state of spore germination.<br />

mushrooms that require the presence of microfbra,<br />

such as the Button Mushroom (Agaricus<br />

brunnescens) and the King Stropharia (Stmpharia<br />

rugoso-annulata) are excluded. The key requirement<br />

is that the parent mushroom fru<strong>it</strong>s on a<br />

sterilized substrate, w<strong>it</strong>hin a sterile environment,<br />

and sporulates abundantly. The following<br />

mushrooms are some of those which qualify.<br />

All are wood or straw saprophytes.<br />

Agrocybe aeger<strong>it</strong>a<br />

Flammulina velutipes<br />

Ganoderma lucidum and allies<br />

Lentinula edodes<br />

Pholiota narneko<br />

Pleurotus c<strong>it</strong>rinopileatus<br />

Pleurotus djamor<br />

Pleurotus eryngii<br />

Pleurotus euosmus<br />

GENERATING GRAIN SPAWN 143<br />

Pleurotus ostreatus<br />

Pleurotus pulmonarius<br />

A practical approach is to first sterilize a halffilled<br />

gallon of wood chips which is then<br />

inoculated w<strong>it</strong>h grain spawn. After several<br />

weeks of incubation, depending on the species,<br />

mushrooms form w<strong>it</strong>hin the environment of the<br />

gallon jar. (Supplementation, for instance w<strong>it</strong>h<br />

rice bran, oatmeal, or rye flour facil<strong>it</strong>ates mushroom<br />

formation.) Once mature, the mushrooms<br />

are aseptically removed and immersed in sterilized<br />

water. Commonly the water is enriched<br />

w<strong>it</strong>h sugar-based nutrients and trace minerals<br />

to encourage rapid spore germination. Millions<br />

of spores are washed into the surrounding broth.<br />

After vigorous shaking (a few seconds to a few<br />

minutes), the spore-enriched liquid is poured<br />

off into another sterile container, creating a<br />

Spore-Mass Master.<br />

Spores begin to germinate w<strong>it</strong>hin minutes of<br />

Figure 117. Pressurizei vessels in China designed<br />

for spore mass fermentation.<br />

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