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CULTURING MUSHROOM MYCELIUM ON AGAR MEDIA 95
When cloning a mushroom for the first time,
I recommend a minimum of 5 repetitions. If the
mushroom specimen is rare, cloning into several
dozen dishes is recommended. As the
specimen dries out, viable clones become increasingly
less likely. With the window of
opportunity for cloning being so narrow, the
cultivator should clone mushrooms within
hours of harvesting. If the mushrooms must be
stored, then the specimen should refrigerated at
F. C.).After three to four days from
harvest, finding viable and clean tissue for cloning
is difficult.
A few days to two weeks after cloning the
mushroom, the tissue fragment springs to life,
becoming fuzzy in appearance. Contaminants
usually become visible at this stage. As a rule,
the cultivator always transfers viable mycelium
away from contamination, not the other way
around. The essential concept here, is that the
cultivator "runs" with the mycelium, subculturing
away from contamination as many times as
is necessary until a pure culture is established.
Each transfer from an older petri dish culture
to a newer petri dish moves upstream. The scalpel
is brought into contact with heat. The tip is
cooled into the dish destined to receive the
mycelium.The lid of this dish is lifted, the scalpel
is cooled, and then the lid is replaced. Next,
the lid of the dish hosting the mature mycelium
is opened. The mycelium is cut. The wedge is
transferred to the newly poured media plate.
With the lid replaced, the culture is labelled and
moved aside. The process is repeated until a
number of plates are inoculated.
As each lid is lifted, care is taken not to extend
the fingers beyond the lip of each top.The
overhanging of fingers results in off-flaking of
contaminants into the petri dish. Furthermore,
the lids are lifted with their undersides catch-
Figure 72. Faul Stamets' simple, etfective 12 ft. long, laminar flow bench designed for commercial cultivation.
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