GROWING GOURMET - Anto2ni.it

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CULTURING MUSHROOM MYCELIUM ON AGAR MEDIA
Malt Extract, Yeast Agar
1000 milliliters (1 liter) water
20 grams agar agar
20 grams barley malt sugar
2 gram yeast (nutritional)
1 gram peptone (optional, soybean derived)
(The above medium is abbreviated as MYA.
With the peptone, which is not critical for most
of the species described in this book, this medium
is designated MYPA.)
Potato, Dextrose, Yeast Agar
1000 milliliters (1 liter) water
300 grams of potatoes (i. e. the broth from boiling
potatoes in 2-3 liters of water for 1 hour)
20 grams agar agar
10 grams dextrose
2 grams yeast
1 gram peptone (optional, soybean derived)
(This medium is designated PDYA, or
PDYPA if peptone is added. Note that only the
broth from boiling the potatoes is used-the potatoes
are discarded. The total volume of the
media should equal 1 liter.)
Oatmeal, Malt, Yeast Enriched Agar
1000 milliliters water (1 liter)
80 grams instant oatmeal
20 grams agar agar
10 grams malt sugar
2 grams yeast
(This rich medium is called OMYA. The
oatmeal does not have to be filtered out although
some prefer to do so.)
Dog Food Agar
1000 milliliters water (1 liter)
20 grams dry food*
20 grams agar agar
* Dog food was first used as a component for agar medium by
the late Dr. Steven Pollock.
Corn Meal, Yeast, Glucose Agar
1000 milliliters water (1 liter)
20 grams agar agar
10 grams cornmeal
5 grams malt or glucose
1 gram yeast
(This medium is known as CMYA and is
widely used by mycological laboratories for
storing cultures and is not as nutritious as the
other above-described formulas.)
The pH of the above media formulations, after
sterilizing, generally falls between 5.5-6. 8.
This media can be further fortified with the addition
of 3-5 grams of the end-substrate (in most
cases hardwood sawdust) upon which mushrooms
willbeproduced. If samples of soil ordung are desired,
they first must be pre-boiled for 1-2 hours
before adding to any of the above formulas. One
potential advantage of the addition of these endsubstrate
components is a significant reduction in
the "lag period. "The lag period is seen when
mushroommyceliumencountersunfarniliarcomponents.
(See Leatham & Griffin, 1984; Raaska
1990). This simple step can greatly accelerate
the mushroom life cycle, decreasing the duration
of colonization prior to fruiting.
The dry components are mixed together,
placed into a flask, to which 1 liter of water is
added. This cultivator finds that well-water,
spring water, or mineral water works well.
Chlorinated water is not recommended. Purchasing
distilled water is unnecessary in my
opinion. Once the media has been thoroughly
mixed, the media flask is placed into a pressure
cooker. The top of the media flask is either
stopped with non-absorbent cotton, wrapped in
aluminum foil, or, if equipped with a screw cap,
the cap is loosely tightened. Sterilize for 45
minutes @ 15 psi or @ 2500 F
Pressure cookers or sterilizers that do not re-
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