Biology 65 Syllabus - Columbia College
Biology 65 Syllabus - Columbia College
Biology 65 Syllabus - Columbia College
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
INTRODUCTION TO MICROBIOLGY<br />
<strong>Biology</strong> <strong>65</strong>--Spring 2013<br />
Lecture T/Th 8:00am-9:25am in Sugar Pine 126<br />
Lab T/Th 9:40am-11:05am (section 0497) or 11:20am-12:45pm( section 0496) in Sugar Pine 207<br />
Instructor information: Helen Clyatt Yost, M.D. email: yosth@yosemite.edu phone: 736-9456<br />
office: adjunct office Tamarack 209, cubicle A hours: Tues 1-2pm and by arrangement<br />
Course Description: This is a college level, transferable, four unit, lab science course in introductory<br />
microbiology. Since many students take this class as a prerequisite for nursing or other health-related<br />
fields, in addition to general principals of microbial identification, growth, culture, and safe handling,<br />
we particularly consider microbes that cause human illness and human defenses against infectious<br />
disease. Aside from disease, however, we hope you'll gain appreciation for microbiologist Mark<br />
Wheelis's statement that "the earth is fundamentally a microbial planet" and marvel at the diversity and<br />
significance of microbial life.<br />
Four themes run through this course:<br />
I. Microbes are incredibly diverse.<br />
II. Microbes are indispensable for continued life on the planet.<br />
III. Humans and microbes interact every day in many ways.<br />
IV. Human activities are contributing to the emergence and spread of infectious diseases.<br />
Texts: Tortora, Funk, and Case Microbiology, an Introduction, (11th edition preferred, 10th ok) is the<br />
required text for this class. Expensive as such textbooks are, this one is worth purchasing to keep as a<br />
reference, especially for anyone entering a health care field. It is readable, highly cross-referenced, and<br />
includes many useful charts and illustrations.<br />
Mark Walters Six Modern Plagues and How We Are Causing Them is required reading. It is written for<br />
a general audience and may be found in a public library.<br />
Leboffe and Pierce A Photographic Atlas for the Microbiology Laboratory, also required, has excellent<br />
photos and background information both for laboratory work and some of the lecture topics. .<br />
George Sackheim An Introduction to Chemistry for <strong>Biology</strong> Students is useful for its teach-yourself<br />
presentation of chemistry fundamentals important in the biological sciences, and is an optional text.<br />
Each of these books will be available on reserve in the <strong>Columbia</strong> <strong>College</strong> library.<br />
Blackboard: This syllabus, assignments, lecture outlines and notes, slides, study guides, links, and<br />
announcements will be posted throughout the semester on Blackboard. Information on accessing<br />
Blackboard for this class is available on the college home page under Distance Learning--> Student<br />
Resources. Also be sure to activate your student email since that is the way I can reach you.<br />
Grading:<br />
4 lecture/lab exams = 200-325 points A: 90% and above<br />
class assignments = 40-100 points B: 80-89%<br />
lab reports and skills = 115-130 points C: 70-79%<br />
lab skills comprehensive exam= 50-75 points D: 60-69%<br />
comprehensive final= 120-170 points F: less than 60%<br />
total = 525-800 points (approximate point count, and ranges above are approximate)<br />
Late work is discouraged but accepted up until the assignment is handed back to the class. There will<br />
be a twenty percent penalty unless prior arrangement has been made. For true emergencies on exam
days, please leave a voice or email with the instructor as soon as possible. Written documentation (eg.<br />
doctor's note, court papers, funeral notice, police report) is a good way to explain the circumstances.<br />
Make-up exams may be all essay or oral at the instructor's discretion. Assignments and lab reports may<br />
be left in the instructor's box in Manzanita. It's best to notify the instructor if you are doing this.<br />
Attendance: Past experience shows that good attendance at all class sessions makes successful<br />
completion of the course more likely. In the laboratory where skills in safely handling microbes are<br />
learned sequentially, repeated absences will render a student potentially a danger to him/herself and<br />
others. For this reason a student frequently absent from the lab will be dropped from the class.<br />
Lab Schedule and Tentative Lecture Schedule:<br />
Wk Date Lecture Topics (Tortora chapter) Lab Exercises (Leboffe pages)<br />
(T=10th ed pages; E= 11th ed pages)<br />
1 1/15 Intro; History microbiology, overview<br />
microbes; classification (1; 10 T:p.274-282;<br />
E:p.272-281) (Leboffe p. 1<strong>65</strong>-7, archaea)<br />
Intro; Safety; Media; Handwashing<br />
(p.1- 4)<br />
1/17 continue microbiology history Preparation of media, sterilization<br />
2 1/22 review chemistry basics: macromolecules,<br />
reactions, pH, ATP (2)<br />
1/24 Microscopy (3)(Leboff p. 31-36)<br />
biofilms (T:p. 162-163; E: p. 160-161)<br />
3 1/29 Cell Structure and Function--Prokaryotes (4)<br />
current event #1 due<br />
1/31 Cell Structure and Function--Procaryotes and<br />
Eukaryotes (4)<br />
Microscope use (p.31-36)<br />
Bring pond water Wet mounts--water and<br />
termite gut microbes (p.55; p.170-176)<br />
Bring interesting food sample Ubiquitous<br />
microbes; selective, differential media<br />
(p.5-6, 11-12 ); continue wet mounts as<br />
needed; aseptic transfer practice<br />
4 2/5 Exam #1<br />
Evaluate environmental plates; (p.19-27)<br />
streak plates for isolation (p.5-6)<br />
Simple Stain--prokaryotes and eukaryotes<br />
(p. 37-44); evaluate streak plates ;<br />
evaluate Sab plates<br />
2/7 Metabolism: enzymes, oxidation-reduction,<br />
ATP (5)<br />
Gram Stain (p.45-48)<br />
5 2/12 Metabolism: carbohydrate catabolism, aerobic<br />
and anaerobic (5)<br />
2/14 Metabolism: Fermentation; Fermentation<br />
technology; photosynthesis<br />
(5; 28 T:p. 797-800; E: 805-807)<br />
6 2/19 Metabolic Diversity; nitrogen and sulfur cycles<br />
(5; 11 T:p.313-315.E: p. 320-324;<br />
27 T: 768-774; E: P. 776-780)<br />
2/21 wrap up metabolism and cycles<br />
Microbial Growth Requirements(6)<br />
Negative Stain (p. 38); aseptic transfers;<br />
dilution theory (p.217-219)<br />
Milk analysis, Bring sample<br />
Evaluate quantitative milk plates;<br />
Endospore Stain (p.51-54)<br />
Anaerobic cultures (p.27-30)
7 2/26 Exam #2 Evaluate anaerobic cultures and subculture<br />
gaspak organism;<br />
set up fungal slide cultures (p. 178-182)<br />
lecture in lab<br />
2/28 Control of microbial growth:<br />
(7: 28 T:p.794-797; E: 800-804 )<br />
8 3/5 Microbial Genetics: DNA structure and<br />
replication (8)<br />
current event #2 due<br />
3/7 Genetic expression, transfer, mutation (8)<br />
(Leboffe p. 114-116)<br />
Evaluate anaerobic subculture;<br />
Set up Chemical Control test;<br />
Bring samples (p.223-5)<br />
Evaluate chemical control plates and<br />
subculture; evaluate fungal slide cultures,<br />
prepare yeast wet mounts (p. 178-182)<br />
continue fungal slides, wet mounts as<br />
needed; evaluate chemical control<br />
subculture; Water analysis (p.11; 228)<br />
Tortora (T:p, 779-81; E: 784-787)<br />
Bring water samples<br />
9 3/12 continue microbial genetics evaluate water analysis plates;<br />
Video: When Wonder Drugs Don't Work;<br />
MRSA detection (p.14)<br />
3/14 continue microbial genetics<br />
Viruses (13)<br />
(Leboffe p. 117-122)<br />
evaluate MRSA plates<br />
Acid-fast Stain (p.49-50)<br />
(Tortora T:p.594, 619-620, 682-686;<br />
E: p. 599, 625-626,688-691)<br />
10 3/19 Exam #3 Set up Plaque Assay for phage count<br />
(p. 220-221; 117-119)<br />
3/21 Viruses cont. (polio T:p. 621-2; E:626-8;<br />
rabies T: p. 622-4; E: 628-30;<br />
influenza T:p. 692-5; E: 699-701)<br />
11 3/26 continue viruses<br />
Biotechnology and Recombinant DNA (9)<br />
(Leboffe 109-113)<br />
3/28 continue biotechnology<br />
Selected eucaryotes (12)<br />
12 4/2 Normal microbiota; principals of disease and<br />
epidemiology(14; vector-borne examples:<br />
lyme disease T:p.<strong>65</strong>2-4; E: <strong>65</strong>8-60,<br />
plague T:p.648-<strong>65</strong>2; E: <strong>65</strong>5-8)<br />
4/4 Microbial Pathogenicity; Toxins; Food-Borne<br />
Illness (15; tetanus T: p.615; E: 621-2;<br />
botulism T: p.618; E: 622-5;<br />
gastroenteritidies T:p.710-718; E: 716-722)<br />
Set up p-Blu transformation exercise;<br />
evaluate plaque assay<br />
Evaluate transformation exercise;<br />
Streak unknowns for isolation<br />
lecture in lab<br />
Plate pure cultures of unknowns;<br />
Microbial Metabolism: inoculate QRST<br />
organisms for glucose, IMViC, TSI (p.71;<br />
74-5; 82; 98;64-<strong>65</strong>; 95-96)<br />
Identify QRST orgs;<br />
Gram stain unknowns<br />
Inoculate unknowns for sugars, IMViC,<br />
Litmus milk (p. 78-80), TSI
13 4/9 Exam #4<br />
(includes Six Modern Plagues)<br />
Other special media and tests:(p. 96-97; 73;<br />
15; 63-64; 14; 70-71) Evaluate IMViC,<br />
sugars, TSI for unknowns<br />
4/11 Host Defenses (16,17) Continue unknowns; Parasites (p193-216)<br />
14 4/16 Host Defenses (16,17)<br />
4/18 Host Defenses; Vaccines; Monoclonal<br />
Antibodies (16, 17, 18)<br />
15 4/23 Microbial ID and diagnostics (10 T: p.282-294;<br />
E: 281-294 18 T: p.509-518; E: 511-523<br />
Leboffe p. 98-108)<br />
4/25 Hepatitis (T:p.721-8; E: 729-32);<br />
STI's (T:p.744-761; E: 754-7<strong>65</strong>)<br />
16 4/30 Comprehensive Final<br />
8 am-10 am<br />
Set up Nose and Throat Cultures (p.61,<strong>65</strong>-6)<br />
Evaluate Nose and Throat Cultures<br />
(p.61; <strong>65</strong>-66)<br />
finish up and review<br />
Lab Skills Comprehensive Exam<br />
<strong>Columbia</strong> <strong>College</strong> is committed to providing access to students with disabilities. If you have or<br />
believe you have a condition which requires special accommodations, please see me before the<br />
first exam so that we can talk about your options.<br />
The Student Conduct Code, found in the Student Handbook and college catalog, is the governing<br />
document for this and all <strong>Columbia</strong> <strong>College</strong> classes.<br />
Additional Lab Information: A lab packet will be available at the first lab. This packet and<br />
supplemental handouts form the beginning of a loose-leaf lab notebook to be kept by each student. The<br />
notebook will contain notes on the lab exercises, sketches of microscope observations, notes of<br />
techniques, work in progress, and reports as indicated below . Use lined, unlined, and graph paper as<br />
you wish.<br />
Keeping careful records is an extremely important skill in science, and the notebook may be reviewed<br />
from time to time by the instructor. Formal reports will be derived from these notes as indicated.<br />
Format for reports is specified in the lab packet, and those listed in bold are to be in formal scientific<br />
report format.<br />
All lab work (rough notes, exercises, reports, exams) should be retained in the lab notebook for the<br />
entire semester.
Lab skills and assignments Due dates Points Scores<br />
media calculations and glo-germ evaluation in notebook<br />
microscope report form in notebook<br />
pond water/ termite gut report 2/5 5<br />
environmental plate report with simple stain 2/14 5<br />
dilution problems in notebook<br />
milk analysis report in notebook<br />
streak plate 5<br />
gram stain 5<br />
negative stain (4) or with spirochete (5) 4 or 5<br />
endospore stain 5<br />
acid-fast stain 5<br />
anaerobic culture report 3/12 15<br />
fungal slides (Rhodotorula 3, Rhizopus 3, Penicillium 4) 10<br />
chemical control report 3/21 15<br />
plaque assay report 3/28 5<br />
transformation report 4/2 15<br />
unknowns report 4/23 20<br />
nose and throat culture report 4/23 10<br />
(possible notebook review at random 0-10)