2012 Promega catalogue

Cell Signaling
S1 Nuclease
Product Size Conc. Cat.# Price ($)
S1 Nuclease 10,000 u 20–100 u/µl M5761 81.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: S1 Nuclease degrades single-stranded DNA and RNA endonucleolytically
to yield 5´-phosphoryl-terminated products. Double-stranded
nucleic acids (DNA:DNA, DNA:RNA or RNA:RNA) are resistant to degradation
except with extremely high concentrations of enzyme. The enzyme is used
to remove single-stranded termini from double-stranded DNA, for selective
cleavage of single-stranded DNA and for mapping RNA transcripts.
Features:
• Provided with 10X Reaction Buffer: 0.5M sodium acetate
(pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO 4.
Storage Conditions: Store at –20°C.
Additional Enzymes
Single-Stranded DNA Binding Protein
Product Size Conc. Cat.# Price ($)
Single-Stranded DNA Binding Protein 100 µg 1–5 µg/µl M3011 115.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: E. coli Single-Stranded DNA Binding Protein (SSB) consists of
four identical 18.9kDa subunits. It binds with high affinity in a cooperative
manner to single-stranded DNA but does not bind well to double-stranded
DNA. It is involved in DNA replication and in recombination in vivo.
Storage Conditions: Store at –20°C.
Terminal Deoxynucleotidyl Transferase,
Recombinant
Product Size Conc. Cat.# Price ($)
Terminal Deoxynucleotidyl Transferase, 300 u 30 u/µl M1871 113.00
Recombinant
1,500 u 30 u/µl M1875 426.00
Available Separately Size Cat.# Price ($)
Terminal Transferase Buffer Pack
For Laboratory Use.
3 × 500 µl M1893 32.00
Description: Terminal Deoxynucleotidyl Transferase, Recombinant, catalyzes
the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA
initiator accompanied by the release of inorganic phosphate. Single-stranded
DNA is preferred as an initiator. Polymerization is not template-dependent.
The addition of 1mM Co 2+ (as CoCl 2) in the reaction buffer allows the tailing
of 3´-ends with varying degrees of efficiency.
Features:
• Tails Any Type of 3´ End: The presence of 1mM CoCl 2 in the reaction
buffer allows the tailing of any type of 3´ end (3´ and 5´ overhangs or blunt
ends).
• Tested for Apoptotic DNA Labeling: Each lot of enzyme is qualified for
success in the procedure outlined in the DeadEnd Fluorometric TUNEL
System Technical Bulletin #TB235.
• Provided with 5X Reaction Buffer: 500mM cacodylate buffer
(pH 6.8 at 25°C), 5mM CoCl 2, 0.5mM DTT.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol Part#
Terminal Deoxynucleotidyl Transferase, Recombinant Protocol 9PIM187
For complete and up-to-date product information visit: www.promega.com/catalog
AgarACE Enzyme
Product Size Conc. Cat.# Price ($)
AgarACE Enzyme 25 u 0.15–0.30 u/µl M1741 100.00
For Laboratory Use.
500 u 0.15–0.30 u/µl M1743 900.00
Description: AgarACE Enzyme is a unique agarose-digesting enzyme
developed by Promega for simple and quantitative recovery of intact DNA or
RNA from agarose gels. The enzyme is sufficiently thermostable that low-
melting-point (LMP) agarose melted at 65–75°C does not have to be
equilibrated to the reaction temperature before hydrolysis. Simply place the
tube containing the molten agarose at 42–47°C and add AgarACE Enzyme.
With slight modifications to the protocol, AgarACE Enzyme also can
effectively hydrolyze non-LMP agaroses, although the higher temperatures
required to melt them may be deleterious to the DNA or RNA. Finally,
AgarACE Enzyme does not require buffer exchange in order to exhibit high
agarose hydrolytic activity; its activity does not appreciably change in TAE or
TBE buffers used in normal electrophoretic procedures.
Features:
• Fast: Hydrolyzes 200mg of 1% LMP agarose in 15 minutes.
• Convenient: No buffer equilibration step required.
• Performance Tested: Nucleic acids recovered using AgarACE Enzyme
are ready for direct use in a number of procedures such as ligation,
random primer labeling, PCR and sequencing.
Storage Conditions: Store at –20°C.
Protocol Part#
AgarACE Agarose-Digesting Enzyme Technical Bulletin TB228
Topoisomerase I
Product Size Conc. Cat.# Price ($)
Topoisomerase I 200 u 2–10 u/µl M2851 197.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Topoisomerase I, isolated from wheat germ, is an enzyme
capable of removing negative supercoils from covalently closed circular DNA.
Storage Conditions: Store at –70°C.
95
4
Cloning and DNA Markers
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