2012 Promega catalogue

Life
Science
Catalog
2012
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Table of
Contents
Cell Signaling
94
DNA 5´ End-Labeling System
Product Size Cat.# Price ($)
DNA 5´ End-Labeling System 10 reactions U2010 144.00
For Laboratory Use.
Description: The DNA 5´ End-Labeling System is a complete system for
phosphorylating both double- and single-stranded DNA and RNA with T4
Polynucleotide Kinase and [γ- 32 P]ATP. The system includes enzymes, buffers
and control DNA standards to measure reaction efficiencies. Calf Intestinal
Alkaline Phosphatase is included for removal of the 5´-phosphate prior to
labeling with T4 Polynucleotide Kinase.
Features:
• Convenient: Can use to label both single-stranded and double-stranded
DNA and RNA.
• Complete: System includes enzymes, buffers and control DNA standards
for measuring reaction efficiencies (except radionucleotides).
• Flexible: Works with [γ- 32 P]ATP, [γ- 33 P]ATP or [γ- 35 S]ATP.
Storage Conditions: Store at –20°C.
Protocol Part#
DNA 5´ End-Labeling System Technical Bulletin TB096
Prime-a-Gene ® Labeling System
Product Size Cat.# Price ($)
Prime-a-Gene ® Labeling System
Available Separately
30 reactions U1100 282.00
Nuclease-Free Water 150 ml P1195 125.00
Labeling 5X Buffer 300 µl U1151 58.00
U1100, U1151 For Laboratory Use. P1195 For Research Use Only. Not for Use in Diagnostic
Procedures.
Description: The Prime-a-Gene ® Labeling System provides a complete set
of complementary reagents, including Labeling 5X Buffer that contains random
synthetic hexadeoxynucleotide primers for random-primed labeling of linear
template DNA with radionucleotides. As little as 25ng of input DNA can be used
to generate probes with specific activities >1 × 10 9 cpm/μg.
Storage Conditions: Store at –20°C.
Protocol Part#
Prime-a-Gene ® Labeling System Technical Bulletin
TB049
Ribonuclease H
Product Size Conc. Cat.# Price ($)
Ribonuclease H 50 u 0.5–2 u/µl M4281 207.00
For Laboratory Use.
250 u 0.5–2 u/µl M4285 884.00
Description: Ribonuclease H (RNase H) is an endonuclease that specifically
hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce
3´-OH and 5´-P-terminated products. It will not degrade single-stranded nucleic
acids, double-stranded DNA or double-stranded RNA.
Storage Conditions: Store at –20°C.
RNase ONE Ribonuclease
Product Size Conc. Cat.# Price ($)
RNase ONE Ribonuclease 1,000 u 5–10 u/µl M4261 177.00
For Laboratory Use.
5,000 u 5–10 u/µl M4265 665.00
Description: RNase ONE Ribonuclease is a 27kDa periplasmic enzyme
from E. coli that catalyzes the degradation of RNA to cyclic nucleotide mono-
phosphate (NMP) intermediates. Slower hydrolysis further catalyzes the
degradation of these intermediates to 3´-NMPs. RNase ONE Ribonuclease is
one of the few known RNases that can cleave a phosphodiester bond between
any two ribonucleotides. RNase ONE Ribonuclease may be used to remove
RNA from DNA preparations, for RNase protection assays and for mapping or
quantitation of RNA by selective cleavage of single-stranded regions.
Features:
• Flexible: RNase ONE Ribonuclease has the ability to cleave phosphodiester
bonds between any two ribonucleotides.
• Provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5 at 25°C),
50mM EDTA, 2M sodium acetate.
Storage Conditions: Store at –20°C. Do not freeze at –70°C. Do not store
on dry ice.
RQ1 RNase-Free DNase
Product Size Conc. Cat.# Price ($)
RQ1 RNase-Free DNase
For Laboratory Use.
1,000 u 1 u/µl M6101 86.00
Description: RQ1 RNase-Free DNase is a preparation of deoxyribonuclease I
that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl
oligonucleotides. This preparation is qualified for use in applications where
maintaining the integrity of RNA is critical.
Features:
• Convenient: 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C],
100mM MgSO 4, 10mM CaCl 2) and Stop Buffer (20mM EGTA [pH 8.0 at
25°C]) are provided.
Storage Conditions: Store at –20°C.
Protocol Part#
RQ1 RNase-Free DNase Protocol 9PIM610
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