2012 Promega catalogue

Cell Signaling
Kinases
T4 Polynucleotide Kinase
Product Size Conc. Cat.# Price ($)
T4 Polynucleotide Kinase 100 u 5–10 u/µl M4101 74.00
1,000 u 5–10 u/µl M4103 553.00
Available Separately Size Cat.# Price ($)
T4 PNK Buffer Pack 1.5 ml C1313 32.00
For Laboratory Use.
Description: T4 Polynucleotide Kinase catalyzes the transfer of the
γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mono-
nucleotides bearing a 5´-hydroxyl group. The enzyme, purified from
recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic
oligonucleotides prior to subsequent manipulations such as ligation.
Features:
• May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated
by heating at 68°C for 10 minutes.
• Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C),
100mM MgCl 2, 50mM DTT.
• Blue/White Cloning Qualified: Promega’s blue/white cloning assay
provides a higher level of quality control for enzymes used in cloning
applications.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol Part#
T4 Polynucleotide Kinase Technical Bulletin TB519
Nucleases
Exonuclease III
Product Size Conc. Cat.# Price ($)
Exonuclease III 5,000 u 150–200 u/µl M1811 99.00
For Laboratory Use.
25,000 u 150–200 u/µl M1815 363.00
Description: Exonuclease III is a 3´→5´ exonuclease specific for doublestranded
DNA. The enzyme catalyzes the stepwise removal of mononucleotides
starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs
of less than 4 bases, yielding nucleoside 5´-phosphates. Exonuclease III will
degrade DNA from 3´-phosphate ends due to its intrinsic 3´-phosphatase
activity. In addition, the enzyme has apurinic endonuclease activity and
ribonuclease H activity. Exonuclease III is used in conjunction with S1 nuclease
for unidirectional deletion of sequences from the termini of DNA fragments.
Features:
• Flexible: Control deletion rate by varying incubation temperature.
• May Be Heat-Inactivated: Exonuclease III may be inactivated by heating
to 75°C for 10 minutes.
• Provided with 10X Reaction Buffer: 660mM Tris-HCl (pH 8.0 at 25°C),
6.6mM MgCl 2.
Storage Conditions: Store at –20°C.
Protocol Part#
Exonuclease III Protocol 9PIM181
For complete and up-to-date product information visit: www.promega.com/catalog
Erase-a-Base System
Product
Erase-a-Base System (minus vectors &
Size Cat.# Price ($)
bacterial strain) 1 system E5750 743.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Erase-a-Base System is designed for the rapid
construction of plasmid or M13 subclones containing progressive uni-
directional deletions of any inserted DNA. The system is based on the
procedure developed by Henikoff, in which exonuclease III (Exo III) is used to
specifically digest insert DNA from a 5´ protruding or blunt end restriction site.
The adjacent sequencing primer binding site is protected from digestion by a
4-base 3´ overhang restriction site or by an α-phosphorothioate-filled end.
Features:
• Rapid: Construction of nested deletions from plasmid or M13 clones are
rapid. Construction is complete in a few hours.
• Efficient: Produce deletion sets spanning several kilobases.
Storage Conditions: Store at –20°C.
Protocol Part#
Erase-a-Base ® System Technical Manual
TM006
Mung Bean Nuclease
Product Size Conc. Cat.# Price ($)
Mung Bean Nuclease 2,000 u 50–100 u/µl M4311 152.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Mung Bean Nuclease catalyzes the degradation of singlestranded
DNA and RNA endonucleolytically to yield 5´-phosphoryl-terminated
products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at
very high concentrations the enzyme degrades double-stranded DNA from both
ends. Mung Bean Nuclease has been used for transcript mapping studies, for
flushing staggered ends and for the separation of cDNA strands after synthesis
with reverse transcriptase and DNA Polymerase I.
Features:
• Provided with 10X Reaction Buffer: 300mM sodium acetate
(pH 5.0 at 15°C), 500mM NaCl, 10mM ZnCl 2.
Storage Conditions: Store at –20°C.
Protocol Part#
Mung Bean Nuclease Protocol 9PIM431
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Cloning and DNA Markers
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