2012 Promega catalogue

Life
Science
Catalog
2012
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Table of
Contents
Cell Signaling
Ligases
92
LigaFast Rapid DNA Ligation System
Product Size Cat.# Price ($)
LigaFast Rapid DNA Ligation System 30 reactions M8221 113.00
150 reactions M8225 371.00
Available Separately
2X Rapid Ligation Buffer
For Laboratory Use.
1.5 ml C6711 156.00
Description: The LigaFast Rapid DNA Ligation System is designed for
the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just
5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is
based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation
Buffer. The LigaFast System is designed to eliminate any further purification
prior to transformation of ligated DNA. The specially formulated 2X Rapid
Ligation Buffer requires no additional ATP or Mg 2+ addition prior to use.
Features:
• Flexible: Use with 5´, 3´ or blunt-ended DNA inserts.
• Fast: Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes
at room temperature.
• Convenient: No requirement to purify ligated DNA prior to heat-shock
transformation in E. coli. Ligations conducted at room temperature.
• Ready-To-Use: No additional buffer modifications required prior to use.
• Efficient: Ligations performed using the LigaFast System are
comparable to standard overnight ligations.
• Blue/White Cloning Qualified: Promega’s blue/white cloning assay
provides a higher level of quality control for enzymes used in cloning
applications.
Storage Conditions: Store at –20°C.
Protocol Part#
LigaFast Rapid DNA Ligation System Protocol 9PIM822
T4 DNA Ligase
Product Size Conc. Cat.# Price ($)
T4 DNA Ligase 100 u 1–3 u/µl M1801 67.00
500 u 1–3 u/µl M1804 242.00
T4 DNA Ligase (HC) 500 u 10–20 u/µl M1794 242.00
Available Separately Size Cat.# Price ($)
T4 DNA Ligase Buffer Pack
For Laboratory Use.
1.5 ml C1263 32.00
Description: T4 DNA Ligase catalyzes the joining of two strands of DNA
between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides
in either a cohesive-ended or blunt-ended configuration. The enzyme has also
been shown to catalyze the joining of RNA to either a DNA or RNA strand in a
duplex molecule but will not join single-stranded nucleic acids.
The T4 DNA Ligase Buffer Pack includes 3 tubes of T4 DNA Ligase 10X
Reaction Buffer. The composition of the 10X reaction buffer is 300mM Tris-HCl
(pH 7.8 at 25°C), 100mM MgCl 2, 100mM DTT and 10mM ATP.
Features:
• Available at High Concentration: Cat.# M1794 contains 500 units of
T4 DNA Ligase at 10–20u/μl.
• Flexible: Use with 5´, 3´ or blunt-ended DNA inserts.
• Provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C),
100mM MgCl 2, 100mM DTT and 10mM ATP.
• Blue/White Cloning Qualified: Promega’s blue/white cloning assay
provides a higher level of quality control for enzymes used in cloning
applications.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol Part#
T4 DNA Ligase, Blue/White Cloning Qualified Protocol 9PIM180
T4 RNA Ligase
Product Size Conc. Cat.# Price ($)
T4 RNA Ligase 500 u 10 u/µl M1051 152.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: T4 RNA Ligase catalyzes the ATP-dependent ligation of singlestranded
RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA
or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient),
has an apparent molecular weight of 43.5kDa. T4 RNA Ligase also catalyzes
the addition of [5´- 32 P] nucleoside 3´,5´-bis (phosphate) onto single-stranded
RNA.
Features:
• May Be Heat-Inactivated: T4 RNA Ligase may be inactivated by heating
at 65°C for 15 minutes.
• Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.8 at 25°C),
100mM MgCl 2, 50mM DTT, 10mM ATP.
Storage Conditions: Store at –20°C.
Protocol Part#
T4 RNA Ligase Protocol 9PIM105
For complete and up-to-date product information visit: www.promega.com/catalog