2012 Promega catalogue

Life
Science
Catalog
2012
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Cell Signaling
90
DNA Polymerase I Large (Klenow) Fragment
Mini Kit
Product Size Cat.# Price ($)
DNA Polymerase I Large (Klenow)
Fragment Mini Kit 150 u U1300 128.00
Available Separately
DNA Polymerase I Large (Klenow)
Size Conc. Cat.# Price ($)
Fragment
Set of dATP, dCTP,
150 u 5 u/µl M2201 117.00
dGTP, dTTP
For Laboratory Use.
10µmol each 100 mM U1330 110.00
Description: The DNA Polymerase I Large (Klenow) Fragment Mini Kit
provides a convenient combination of polymerase and dNTPs. The kit
contains 5μmol each of dATP, dGTP, dTTP and dCTP (10mM in water) and
DNA Polymerase I Large (Klenow) Fragment, ready for use in a variety of
applications.
Features:
• Convenient: The kit provides DNA Polymerase I Large (Klenow) Fragment
and dNTPs conveniently packaged and ready to use in your application.
Storage Conditions: Store at –20°C.
DNA Polymerase I Large (Klenow) Fragment,
Exonuclease Minus
Product Size Conc. Cat.# Price ($)
Klenow Fragment, Exonuclease Minus
For Laboratory Use.
100 u 5–10 u/µl M2181 100.00
Description: DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus,
is a DNA-dependent DNA polymerase that lacks both the 5´→3´ and the 3´→5´
exonuclease activities present in intact E. coli DNA Polymerase I. It is used for
random primer labeling and in strand displacement amplification. Klenow
Fragment, Exonuclease Minus, will leave a single-base 3´ overhang on a
significant proportion of DNA fragments during fill-in of 5´-overhangs.
Therefore, this enzyme is not recommended for preparation of blunt-ended
fragments for ligation.
Features:
• Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.2 at 25°C),
100mM MgSO 4, 1mM DTT.
• May Be Heat-Inactivated: DNA Polymerase I Large (Klenow) Fragment,
Exonuclease Minus, is inactivated by heating at 75°C for 10 minutes.
Storage Conditions: Store at –20°C.
Protocol Part#
DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus
Protocol
9PIM218
T4 DNA Polymerase
Product Size Conc. Cat.# Price ($)
T4 DNA Polymerase 100 u 5–10 u/µl M4211 140.00
For Laboratory Use.
500 u 5–10 u/µl M4215 529.00
Description: T4 DNA Polymerase catalyzes the 5´→3´ synthesis of DNA from
a primed single-stranded DNA template. Although possessing a potent 3´→5´
proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease
activity. T4 DNA Polymerase can be used to fill 5´ protruding ends with labeled
or unlabeled dNTPs or for the generation of blunt ends from DNA molecules
with 3´ overhangs.
Features:
• High Fidelity: T4 DNA Polymerase is the enzyme of choice for applications
where misincorporation is a concern.
• Flexible: T4 DNA Polymerase may be used in a variety of molecular
applications. Active in many Promega 1X restriction enzyme buffers.
• May Be Heat-Inactivated: T4 DNA Polymerase is inactivated by heating
at 75°C for 10 minutes.
• Provided with 10X Reaction Buffer: 250mM Tris-acetate (pH 7.7),
1M potassium acetate, 100mM magnesium acetate and 10mM DTT.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol Part#
T4 DNA Polymerase Protocol 9PIM421
SP6 RNA Polymerase
Product Size Conc. Cat.# Price ($)
SP6 RNA Polymerase 1,000 u 10–20 u/µl P1085 259.00
5,000 u 10–20 u/µl P1081 971.00
SP6 RNA Polymerase (HC)
For Laboratory Use.
2,500 u 80 u/µl P4084 539.00
Description: SP6 RNA Polymerase is a DNA-dependent RNA polymerase that
exhibits extremely high specificity for its cognate promoter sequences. Only
SP6 DNA or DNA cloned downstream from an SP6 promoter can serve as a
template for SP6 RNA Polymerase-directed RNA synthesis.
Features:
• Specific: SP6 RNA Polymerase exhibits extremely high affinity and
specificity for SP6 promoter sequences.
• Highly Pure: SP6 RNA Polymerase is >90% pure as determined by
SDS polyacrylamide gel electrophoresis. Free of detectable levels of
contaminating RNase and DNase activity (