2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
88
XmaI
Product Size Conc. Cat.# Price ($)
XmaI 50 u 1–5 u/µl R6491 110.00
For Laboratory Use.
Description:
C ▼ CCGG G
G GGCC ▲C
Storage Conditions: Store at –20°C.
XmnI
37
250 u 1–5 u/µl R6495 414.00
Product Size Conc. Cat.# Price ($)
XmnI 500 u 10 u/µl R7271 81.00
For Laboratory Use.
2,500 u R7273 296.00
Description:
GAANN ▼ NNT TC
CT TNN ▲NNAAG
Storage Conditions: Store at –20°C.
MULTI-CORE Buffer Pack
Product Size Cat.# Price ($)
MULTI-CORE Buffer Pack
For Laboratory Use.
3 × 1 ml R9991 22.00
Description: The MULTI-CORE Buffer Pack contains convenient aliquots
of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE
Buffer is formulated to provide simple buffering conditions for performing
multiple digestions. Many Promega restriction enzymes have between 50%
and 100% activity in reactions using MULTI-CORE Buffer.
Features:
• Convenient and Economical: MULTI-CORE Buffer enables codigestion
of DNA with more than one enzyme in a single reaction. In most
cases, only modest adjustments in the amount of enzyme used will ensure
complete multiple digestions.
Storage Conditions: Store at –20°C.
37
4-CORE ® Buffer Pack
Product Size Cat.# Price ($)
4-CORE ® Buffer Pack (Buffers A, B, C and D), 1ml
each
For Laboratory Use.
4 ml R9921 22.00
Description: The 4-CORE ® Buffer Pack contains convenient aliquots of
Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of
Promega restriction enzymes have optimal activity in one of these four 10X
reaction buffers.
Storage Conditions: Store at –20°C.
Alkaline Phosphatases
Alkaline Phosphatase, Calf Intestinal (CIAP)
Product Size Conc. Cat.# Price ($)
Alkaline Phosphatase, Calf Intestinal 1,000 u 1 u/µl M1821 107.00
Alkaline Phosphatase, Calf Intestinal (HC) 1,000 u 20 u/µl M2825 107.00
Available Separately Size Cat.# Price ($)
CIAP Buffer Pack
For Laboratory Use.
1.5 ml M1833 32.00
Description: Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the
hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside
triphosphates. This enzyme is used to prevent recircularization
and religation of linearized cloning vector DNA by removing phosphate groups
from both 5´-termini and may also be used for the dephosphorylation of 5´
phosphorylated ends of DNA or RNA for subsequent labeling with [ 32 P]ATP and
T4 Polynucleotide Kinase. CIAP is active on 5´ overhangs, 5´ recessed and blunt
ends.
Features:
• Available at High Concentration: Cat.# M2825 contains 1,000 units of
CIAP at 20u/μl.
• Blue/White Cloning Qualified: Promega’s blue/white cloning assay
provides a higher level of quality control for enzymes used in cloning
applications.
• Provided with 10X Reaction Buffer: 0.5M Tris-HCl (pH 9.3 at 25°C),
10mM MgCl 2, 1mM ZnCl 2, 10mM spermidine.
Storage Conditions: Store at –20°C.
Protocol Part#
Alkaline Phosphatase, Calf Intestinal (CIAP) Protocol 9PIM182
For complete and up-to-date product information visit: www.promega.com/catalog