2012 Promega catalogue

Cell Signaling
Blue/Orange Loading Dye, 6X
Product Size Cat.# Price ($)
Blue/Orange Loading Dye, 6X 3 ml G1881 60.00
For Laboratory Use.
Description: Blue/Orange Loading Dye, 6X, is a convenient marker dye
containing 0.4% orange G, 0.03% bromophenol blue, 0.03% xylene cyanol
FF, 15% Ficoll ® 400, 10mM Tris-HCl (pH 7.5) and 50mM EDTA (pH 8.0). It is
provided in a premixed, ready-to-use form. The dye is used for loading DNA
samples into gel electrophoresis wells and tracking migration during electrophoresis.
In a 0.5–1.4% agarose gel in 0.5X TBE, xylene cyanol FF migrates
at approximately 4kb, bromophenol blue at approximately 300bp and orange G
at approximately 50bp.
Features:
• Quality Tested: Each lot of Blue/Orange Loading Dye, 6X, is tested and
certified to be free of nuclease activity.
Storage Conditions: Store at –20°C.
Boric Acid, Molecular Biology Grade
(orthoboric acid)
Product Size Cat.# Price ($)
Boric Acid, Molecular Biology Grade 500 g H5001 46.00
For Research Use Only. Not for Use in Diagnostic Procedures.
1 kg H5003 87.00
Description: Boric Acid, Molecular Biology Grade, in conjunction with Tris, is
commonly used in buffers for the preparation of agarose or acrylamide gels
and their associated running buffers.
Formula Weight: 61.84.
Properties:
• Purity: ≥99.5%.
• Iron: ≤5ppm.
• Lead: ≤5ppm.
• Moisture: ≤0.5%.
• A 260 at 1M: ≤0.015.
• A 280 at 1M: ≤0.010.
Features:
• Quality Tested: Each lot of Boric Acid is tested and certified to be free of
DNase, RNase and protease activity.
Storage Conditions: Store at 22–25°C.
Bovine Serum Albumin, Acetylated
Product Size Conc. Cat.# Price ($)
Bovine Serum Albumin, Acetylated 1 ml 10 mg/ml R3961 40.00
For Laboratory Use.
400 µl 1 µg/µl R9461 13.00
Description: Bovine Serum Albumin, Acetylated, can be used as an enzyme
stabilizer or as a carrier protein. It is prepared by a modification of the method
of Gonzalez et al. and dialyzed extensively with deionized water to remove
impurities.
Features:
• Quality Tested: Each lot of BSA is tested and certified to be free of DNase
and RNase activity.
Storage Conditions: Store at –20°C.
Coelenterazines
For complete and up-to-date product information visit: www.promega.com/catalog
Product Size Cat.# Price ($)
Coelenterazine 250 µg S2001 199.00
Coelenterazine-h
For Research Use Only. Not for Use in Diagnostic Procedures.
250 µg S2011 204.00
Description: Luciferases from Renilla, Aequorea and other marine organisms
are commonly used as indicators or reporters for studying cellular phenomena
in expression assays in eukaryotic cells. Renilla luciferase is often used as a
reporter of transcription regulation, whereas apoaequorin is often used as a
calcium indicator. Other uses of coelenterazines include chemiluminescent
detection of Reactive Oxygen Species (ROS) in cells or tissues. Promega offers
the following coelenterazine analogs.
Coelenterazine (native) is the luminescent substrate for Renilla luciferase
and apoaequorin. Formula: C 26H 21N 3O 3. Formula Weight: 423.5. Form: Film.
Coelenterazine-h imparts a luminescent intensity with its aequorin complex
that is reported to be 10–20 times higher than that of native coelenterazine,
making this derivative a useful tool for measuring small changes in Ca 2+
concentrations. Formula: C 26H 21N 3O 2. Formula Weight: 407.5. Form: Film.
Features:
• Highly Pure: 95%.
• Custom Capabilities: Custom packaging and sizes available.
• Easy to Prepare: Supplied as a dried substrate for easy preparation in
methanol or ethanol.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
DTT, Molecular Grade (DL-Dithiothreitol)
Product Size Conc. Cat.# Price ($)
DTT, Molecular Grade 100 µl 100 mM P1171 47.00
DTT, Molecular Grade (Dry Powder) 5 g V3151 97.00
25 g V3155 362.00
P1171 For Laboratory Use. V3151, V3155 For Research Use Only. Not for Use in Diagnostic
Procedures.
Description: DTT, Molecular Grade, is an antioxidant used to stabilize
enzymes and other proteins containing sulfhydryl groups. The liquid form
of the product is a 100mM solution of DTT in water.
Formula: C 4H 10O 2S 2.
Formula Weight: 154.25.
Form: White crystals/powder or liquid in deionized water.
Properties:
• Purity: ≥99.0%.
• Melting Point: 40–44°C.
• A 283 at 20mM: ≤0.04.
• % Oxidized: ≤0.50%.
Features:
• Quality Tested: Each lot of DTT is tested and certified to be free of DNase,
RNase and protease activity.
Storage Conditions: Store at –20°C.
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Biochemicals and Labware
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