2012 Promega catalogue

Cell Signaling
ProMega-Markers ® Lambda Ladders
Product Size Cat.# Price ($)
ProMega-Markers ® Lambda Ladders 40–60 lanes G3011 198.00
For Laboratory Use.
Description: ProMega-Markers ® Lambda Ladders are prepared by concatemerization
of λ phage DNA into multimers ranging in size from 50kb to
800kb and up, with each multimer, or rung, of the 20-step ladder differing in
size by one λ genome (approximately 48.5kb). The ladders are embedded in
dye-colored, 0.5% agarose string molds in 50mM EDTA. The ladders are not
intended for use in quantitative analysis.
Features:
• Concentration: 0.5μg/5mm.
• Range (bp): 50,000–800,000 and up.
Storage Conditions: Store at 4°C. Do not freeze.
0080TA04_3A
kb
– 800
– 500
– 400
– 200
– 50
0.6% agarose
RNA Markers
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Product Size Cat.# Price ($)
RNA Markers 50 µl G3191 198.00
For Laboratory Use.
Description: Promega RNA Markers are suitable for size estimation of singlestranded
RNA from 0.28–6.58kb in glyoxal or formaldehyde-agarose gels. The
RNA Markers consist of a ladder of nine RNA transcripts that are synthesized
in vitro from specific templates. The sizes are 281, 623, 955, 1,383, 1,908,
2,604, 3,638, 4,981 and 6,583 bases. The markers are not intended for use in
quantitative analysis. After electrophoresis, the fragments can be visualized by
ethidium bromide staining.
Recommended Loading: 3μl (prepared in formaldehyde/MOPS buffer and
separated onto a 1% formaldehyde-agarose gel using MOPS running buffer).
Features:
• Range (bases): 281–6,583.
• Number of Bands: 9.
Storage Conditions: Store at –70°C.
Protocol Part#
RNA Markers Protocol 9PIG319
0775TB09_4A
bases
– 6,583
– 4,981
– 3,638
– 2,604
– 1,908
– 1,383
– 955
– 623
– 281
1% formaldehyde-agarose
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Cloning and DNA Markers
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