2012 Promega catalogue

Life
Science
Catalog
2012
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Contents
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Contents
Cell Signaling
76
ΦX174 DNA/HinfI Dephosphorylated Markers
Product Size Cat.# Price ($)
ΦX174 DNA/HinfI Dephosphorylated Markers 2.5 µg E3511 111.00
For Laboratory Use.
Description: ΦX174 DNA/HinfI Dephosphorylated Markers are prepared
by digesting double-stranded ΦX174 DNA to completion with HinfI. The DNA
fragments are then treated with calf intestinal alkaline phosphatase,
phenol:chloroform-extracted, ethanol-precipitated and resuspended in
TE buffer, making the markers ready for 5´ end-labeling. The 20 DNA
fragments range in size from 24–726bp. The markers are not intended for
use in quantitative analysis.
This marker is especially convenient for applications such as primer extension,
requiring DNA or RNA size estimations.
Features:
• Concentration: 50μg/ml.
• Range (bp): 24–726.
• Number of Bands: 20.
• Convenient: Ready to label.
Storage Conditions: Store at –20°C.
0265TA05_2A
ΦX174 DNA/HinfI Markers
Cat.# G1751
Load 1µl/lane.
bp
8% acrylamide
–
–––––
726
713
– 553
– 500 427
417
413
– 311
– 249
– 200
–––––
– 151
140
– 118
– 100
– 82
– 66
48
42
– 24 40
Analytical Marker DNA Wide Range
Product Size Conc. Cat.# Price ($)
Analytical Marker DNA Wide Range 2 µg 0.2 µg/µl DG1931 139.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Analytical Marker DNA Wide Range, intended for autoradiography,
provides an evenly spaced distribution of 32 DNA fragments ranging from
702bp to 29,950bp in size and 4 smaller fragments (498, 525, 536 and
645bp) that are generally not visible unless the gel is overexposed. This
marker is composed of a mixture of restriction enzyme digests of λ DNA
(Cat.# D1501) and ΦX174 DNA (Cat.# D1531). The marker is not intended
for use in quantitative analysis.
Storage Conditions: Store at –20°C.
0401TA04_2A
bp
––––––––
– 29,950
24,510
– 20,560
– 17,053
– 15,258
– 13,822
– 12,188
– 10,171
– 8,453
– 7,242
– 6,369
– 5,687
– 5,220
– 4,822
– 4,324
– 3,976
– 3,675
– 3,194
– 2,804
– 2,582
– 2,323
– 2,121
– 1,929
– 1,747
– 1,560/1,503
– 1,371
– 1,264
– 1,079
– 964/962
– 702
0.5% agarose
[645, 536,
525, 498]
For complete and up-to-date product information visit: www.promega.com/catalog