2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
4
Antibiotic G-418 Sulfate
Product Size Cat.# Price ($)
Antibiotic G-418 Sulfate 100mg V7981 52.00
1g V7982 188.00
5g V7983 648.00
Antibiotic G-418 Sulfate Solution 20ml V8091 162.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Antibiotic G-418 Sulfate is an aminoglycosidic antibiotic toxic
to both prokaryotic and eukaryotic cells. It acts by interfering with protein
synthesis and is used as an agent for selection of cultured cells expressing
a gene (i.e., aminoglycoside 3´ phosphotransferase [APH 3´]) that confers
resistance to G-418. The liquid form of the product is in distilled water and
aseptically filtered.
Formula Weight: 692.6 (anhydrous).
Form: White powder.
Physical/Chemical Properties of Powder:
• Appearance: White powder.
• TLC: Single major spot.
• Elemental Analysis: %C = 28.8–36.07; %H = 5.76–7.76;
%N = 6.72–8.41.
• Absorbance: A 280 (1mg/ml) = 0–0.015; A 570 (100mg/ml) = 0–0.1.
• Specific Rotation: +104° to +121°.
Properties Specific to V7981, V7982, V7983:
• Appearance: White powder.
• Hydration Waters: 0–6, as determined from Elemental Analysis.
• Potency: >500μg/mg.
Properties Specific to V8091:
• Potency: 40–60mg/ml.
• Sterility: Aseptically filtered.
Features:
• Sterile: Antibiotic G-418 Sulfate Solution is quality tested for sterility.
Storage Conditions: Store powder at 22–25°C. Store liquid at –20°C.
BCIP/NBT Color Development Substrate
(5-bromo-4-chloro-3-indolyl-phosphate/nitro
blue tetrazolium)
Product Size Cat.# Price ($)
BCIP/NBT Color Development Substrate
For Laboratory Use.
1.25/2.5 ml S3771 123.00
Description: BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in
conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of
alkaline phosphatase activity. Each vial of BCIP is supplied with a vial of NBT.
Preparation of Substrates to Detect Alkaline Phosphatase: For every
5ml of alkaline phosphatase buffer (100mM Tris-HCl [pH 9.0], 150mM NaCl,
1mM MgCl 2), add 33μl NBT and 16.5μl BCIP. Add the NBT first, mix, add the
BCIP, and mix again. Use within 1 hour, and discard any unused solution.
Concentration: BCIP (50mg/ml) in 100% dimethylformamide; NBT (50mg/ml)
in 70% dimethylformamide.
Features:
• Quality Tested: Each lot of BCIP/NBT Color Development Substrate is
tested and qualified for use in blotting.
Storage Conditions: Store at either 4°C or –20°C.
Beetle Luciferin, Potassium Salt
Product Size Cat.# Price ($)
Beetle Luciferin, Potassium Salt 5 mg E1601 125.00
50 mg E1602 607.00
250 mg E1603 1771.00
For Research Use Only. Not for Use in Diagnostic Procedures.
1 g E1605 Pls. Enq.
Description: Luciferase genes from the North American firefly (Photinus
pyralis) and from other beetles are commonly used as reporter genes for
studying transcription regulation in transient assay systems and as markers for
stably transformed eukaryotic cells. Beetle luciferin (also known as d-luciferin)
is synthesized as the monopotassium salt and is a substrate for the beetle
luciferase reporter systems. d-luciferin is provided for those researchers who
prefer to formulate their own assay reagents for monitoring in vitro or in vivo
luciferase activity.
Formula: C 11H 7N 2O 3S 2•K.
Formula Weight: 318.4Da (anhydrous).
Features:
• Formulation: Supplied as a potassium salt for easy preparation in
aqueous buffer.
• Choose Your Configuration: Learn more about our custom options
for this product at: www.promega.com/myway/
Storage Conditions: Store at –70°C.
Bisacrylamide, Molecular Grade (N,N´-
Methylenebisacrylamide)
Product Size Cat.# Price ($)
Bisacrylamide, Molecular Grade 25 g V3141 63.00
For Research Use Only. Not for Use in Diagnostic Procedures.
125 g V3143 223.00
Description: Bisacrylamide, Molecular Grade, is a cross-linking agent used in
the preparation of polyacrylamide gels. This product is tested for its efficiency
in gel polymerization.
Formula Weight: 154.20.
Form: White, free-flowing crystals.
Properties:
• Purity: ≥99.0%.
• Acrylic Acid (CH 2:CHCOOH): ≤0.001%.
• A 290 (1% solution): ≤0.20.
• Magnesium: ≤2ppm.
• Conductivity (2% in water): ≤10μmhos.
Features:
• Quality Tested: Each lot of Bisacrylamide is tested and certified to be free
of DNase, RNase and protease activity.
Storage Conditions: Store at 22–25°C.
For complete and up-to-date product information visit: www.promega.com/catalog