2012 Promega catalogue

Cell Signaling
Conventional DNA Markers
Product Size Conc. Cat.# Price ($)
Lambda DNA/HindIII Markers 100 µg 0.5 µg/µl G1711 81.00
Lambda DNA/EcoRI Markers 100 µg 0.5 µg/µl G1721 81.00
Lambda DNA/EcoRI + HindIII Markers 100 µg 0.5 µg/µl G1731 81.00
ΦX174 DNA/HaeIII Markers 50 µg 1 µg/µl G1761 221.00
ΦX174 DNA/HinfI Markers 50 µg 1 µg/µl G1751 221.00
pGEM ® DNA Markers 50 µg 1 µg/µl G1741 171.00
For Laboratory Use.
Description: The Conventional DNA Digest Markers are created by digesting
either λ DNA, ΦX174 replicative form DNA, or plasmids to completion with
one or more restriction enzymes. The enzymes are heat-inactivated, and the
DNA fragments are either phenol-extracted, then ethanol-precipitated or just
ethanol-precipitated. The precipitated fragments are resuspended in storage
buffer. The markers are not intended for quantitative analysis. Each marker is
supplied with a tube of 6X Blue/Orange Loading Dye.
1258TB09_5A
Lambda DNA/
HindIII Markers
Cat.# G1711
Load 1µl/lane.
bp
– 23,130
– 9,416
– 6,557
– 4,361
– 2,322
– 2,027
[564,
125]
0.7% agarose
1258TA09_5A
Lambda DNA/
EcoRI Markers
Cat.# G1721
Load 1µl/lane.
bp
0.7% agarose
– 21,226
– 7,421
5,804
5,643
– 4,878
– 3,530
Lambda DNA/EcoRI +
HindIII Markers
Cat.# G1731
Load 1µl/lane.
φX174 DNA/
HaeIII Markers
Cat.# G1761
Load 1µl/lane.
For complete and up-to-date product information visit: www.promega.com/catalog
1258TC09_5A
bp
– 21,226
5,148
4,973
– 4,268
– 3,530
– 1,584
– 1,375
– 947
– 831
0.7% agarose
– 2,027
––––––– 1,904
[564,
125]
λ DNA/HindIII Markers: Eight ethanol-precipitated DNA fragments ranging in
size from 125bp to 23,130bp.
λ DNA/EcoRI Markers: Six ethanol-precipitated DNA fragments ranging in
size from 3,530bp to 21,226bp.
λ DNA/EcoRI + HindIII Markers: Thirteen ethanol-precipitated DNA
fragments ranging in size from 125bp to 21,226bp.
ΦX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanol-precipitated
DNA fragments ranging in size from 72bp to 1,353bp.
ΦX174 DNA/HinfI Markers: Twenty ethanol-precipitated DNA fragments
ranging in size from 24bp to 726bp.
pGEM ® DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA
fragments ranging in size from 36bp to 2,645bp. These unique markers are
generated from separate digests of pGEM ® -3 Vector DNA with HinfI, RsaI and
AvaII later combined to form the markers.
Recommended Loading: Load 1μl/lane.
Storage Conditions: Store at –20°C.
1238TA09_5A
bp
– 1,353
– 1,078
– 872
– 603
– 310
– 281
––––– 271
– 234
– 194
– 118
[72]
8% acrylamide
0265TA05_2A
φX174 DNA/
HinfI Markers
Cat.# G1751
Load 1µl/lane.
bp
8% acrylamide
–
–––––
726
713
– 553
– 500 427
417
413
– 311
– 249
– 200
–––––
– 151
140
– 118
– 100
– 82
– 66
48
42
– 24 40
0266TA05_1A
pGEM ® DNA
Markers
Cat.# G1741
Load 1µl/lane.
bp
– 2,645
– 1,605
– 1,198
– 676
– 517
– 460
– 396
– 350
– 222
– 179
– 126
– 75
– 65
– 51
[36]
8% acrylamide
8655TA
75
4
Cloning and DNA Markers
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