2012 Promega catalogue

Life
Science
Catalog
2012
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Cell Signaling
74
DNA Ladders
Product Size Conc. Cat.# Price ($)
PCR Markers 250 µl ~0.06 µg/µl G3161 206.00
100bp DNA Ladder 250 µl 0.13 µg/µl G2101 171.00
1kb DNA Ladder 500 µl 0.1 µg/µl G5711 171.00
For Laboratory Use.
Description: The DNA Ladders are ladders with defined sizes. The ladders
are not intended for use in quantitative analysis. Each ladder is provided with
a tube of 6X Blue/Orange Loading Dye.
PCR Markers: Six bands of equal intensity of 50, 150, 300, 500, 750 and
1,000bp. The PCR Markers may be run on polyacrylamide gels with less
loading volume; however, additional bands may be visible compared to those
visible on agarose gels.
0094TA05_3A
PCR Markers
Cat.# G3161
Load 5µl/lane.
bp
2% agarose
– 1,000
– 750
– 500
– 300
– 150
– 50
0973TC03_5A
2% agarose
100bp DNA Ladder
Cat.# G2101
Load 5µl/lane.
bp
– 1,500
– 1,000
– 900
– 800
– 700
– 600
– 500
– 400
– 300
– 200
– 100
100bp DNA Ladder: Eleven fragments that range in size from 100bp to
1,000bp in 100bp increments with an additional band at 1,500bp. The 500bp
fragment is present at increased intensity for easy identification. The ladder is
dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4
Polynucleotide Kinase, allowing visualization by autoradiography.
1kb DNA Ladder: Thirteen blunt-ended fragments with sizes ranging from
250bp to 10,000bp. The 1,000bp and 3,000bp fragments have increased
intensity relative to the other bands on ethidium bromide-stained agarose gels
for easy identification. All other fragments are of equal intensity. The ladder is
dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4
Polynucleotide Kinase, allowing visualization by autoradiography.
Recommended Loading: Load 5μl/lane.
Storage Conditions: Store at –20°C.
1409TA03_6A
1kb DNA Ladder
Cat.# G5711
Load 5µl/lane.
bp
– 10,000
– 8,000
– 6,000
– 5,000
– 4,000
– 3,000
– 2,500
– 2,000
– 1,500
– 1,000
– 750
– 500
– 250,
253
0.7% agarose
For complete and up-to-date product information visit: www.promega.com/catalog
8654TA