2012 Promega catalogue

Cell Signaling
DNA Step Ladders
Product Size Conc. Cat.# Price ($)
10bp DNA Step Ladder 32.5 µg 0.65 µg/µl G4471 171.00
25bp DNA Step Ladder 100 µg 0.36 µg/µl G4511 171.00
50bp DNA Step Ladder 90 µg 0.34 µg/µl G4521 184.00
100bp DNA Step Ladder 100 µg 1 µg/µl G6951 261.00
200bp DNA Step Ladder 100 µg 1 µg/µl G6961 213.00
1kb DNA Step Ladder 90 µg 0.3 µg/µl G6941 235.00
For Laboratory Use.
Description: The DNA Step Ladders are ladders of defined sizes with exact
incremental steps between bands. The ladders are not intended for use in
quantitative analysis. Each ladder is provided with a tube of 6X Blue/Orange
Loading Dye. The fragments may be stained with ethidium bromide.
10bp DNA Step Ladder: Ten blunt-ended DNA fragments ranging from
10bp to 100bp in exactly 10bp increments. All bands are of approximately
equal intensity with the exception of the 10bp band, which may appear slightly
less intense.
25bp DNA Step Ladder: Twelve DNA fragments ranging from 25bp to 300bp
in 25bp increments. An 1,800bp “backbone” fragment is also visible. The
300bp band is ≈3 times more intense than all other bands.
bp
– 100
––––– 90
– 80
––––– 70
– 60
– 50
– 40
– 30
– 20
– 10
4% agarose
(3% NuSieve ® GTG ®
agarose/1% agarose)
10bp DNA Step Ladder
Cat.# G4471
Load 1µl/lane.
bp
25bp DNA Step Ladder
Cat.# G4511
Load 5µl/lane.
– 300
––––– 275
– 250
––––– 225
– 200
– 175
– 150
– 125
– 100
– 75
– 50
– 25
2% agarose/TAE
50bp DNA Step Ladder
Cat.# G4521
Load 5µl/lane.
bp
– 800
––––– 750
– 700
––––– 650
– 600
––––– 550
– 500
– 450
– 400
– 350
– 300
– 250
– 200
– 150
– 100
2% agarose/TAE
For complete and up-to-date product information visit: www.promega.com/catalog
– 50
8652TA
50bp DNA Step Ladder: Sixteen DNA fragments ranging from 50bp to 800bp
in 50bp increments plus an 1,800bp “backbone” fragment. All bands except
the 800bp band are of equal intensity; the 800bp band is ≈3 times more
intense.
100bp DNA Step Ladder: Forty blunt-ended DNA fragments ranging from
100bp to 4,000bp in 100bp increments between 100–2,000bp and in 200bp
increments between 2,000–4,000bp. Two internal features facilitate band
identification. First, a high-intensity 500bp band stands out at the lowest
segment of the ladder (