2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Molecular Weight Markers
72
BenchTop DNA Markers
Product Size Cat.# Price ($)
BenchTop ΦX174 DNA/HaeIII Markers 250 µl G7511 239.00
BenchTop pGEM ® DNA Markers 250 µl G7521 186.00
BenchTop PCR Markers 300 µl G7531 239.00
BenchTop 1kb DNA Ladder 600 µl G7541 189.00
BenchTop 100bp DNA Ladder 300 µl G8291 183.00
For Laboratory Use.
Description: The BenchTop DNA Markers offer the convenience of storage
at room temperature (22–25°C) as well as the capability of direct loading onto
agarose gels. The BenchTop DNA Markers are supplied in a stabilizing solution
of 1X Blue/Orange Loading Dye, which circumvents any requirements for
further manipulation.
BenchTop ΦX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanolprecipitated
DNA fragments ranging in size from 72bp to 1,353bp.
BenchTop pGEM ® DNA Markers: Fifteen phenol-extracted, ethanol-precipitated
DNA fragments ranging in size from 36bp to 2,645bp. These unique
markers are generated from separate digests of pGEM ® -3 Vector DNA with
HinfI, RsaI and SinI later combined to form the markers.
BenchTop PCR Markers: Six bands of equal intensity of 50, 150, 300, 500,
750, and 1,000bp. The BenchTop PCR Markers may be run on polyacrylamide
gels with less loading volume; however, additional bands may be visible compared
to those visible on agarose gels.
2% agarose
bp
– 1,353
– 1,078
– 872
– 603
BenchTop φX174
DNA/HaeIII Markers
Cat.# G7511
Load 5µl/lane.
– 310
– 281/271
– 234
– 194
– 118
– 72
2% agarose
BenchTop pGEM ®
DNA Markers
Cat.# G7521
Load 5µl/lane.
bp
– 2,645
– 1,605
– 1,198
– 676
– 517
– 460
– 396
– 350
– 222
– 179
– 126
– 75/65
[51,36]
bp
2% agarose
BenchTop
PCR Markers
Cat.# G7531
Load 6µl/lane.
BenchTop 1kb DNA Ladder: Thirteen blunt-ended fragments with sizes
ranging from 250bp to 10,000bp. The 1,000bp and 3,000bp fragments have
increased intensity relative to the other bands on ethidium bromide-stained
agarose gels for easy identification. All other fragments are of equal intensity.
The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes
using T4 Polynucleotide Kinase, allowing visualization by autoradiography.
BenchTop 100bp DNA Ladder: Eleven fragments that range in size from
100bp to 1,000bp in 100bp increments with an additional band at 1,500bp.
The 500bp fragment is present at increased intensity for easy identification.
The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes
using T4 Polynucleotide Kinase, allowing visualization by autoradiography.
Recommended Loading: Cat.# G7511, G7521: Load 5μl/lane. Cat.# G7531,
G7541, G8291: Load 6μl/lane.
Features:
• Convenient: Storage at 22–25°C.
• Efficient: Premixed with loading buffer. Ready to load onto agarose gels.
• Versatile: Five different BenchTop DNA Markers available.
Storage Conditions: Store at 22–25°C.
– 1,000
– 750
– 500
– 300
– 150
– 50
bp
– 10,000
– 8,000
– 6,000
– 5,000
– 4,000
– 3,000
– 2,500
– 2,000
– 1,500
– 1,000
– 750
– 500
– 250,
253
0.7% agarose
BenchTop 1kb
DNA Ladder
Cat.# G7541
Load 6µl/lane.
2% agarose
For complete and up-to-date product information visit: www.promega.com/catalog
bp
– 1,500
– 1,000
– 900
– 800
– 700
– 600
– 500
– 400
– 300
– 200
– 100
BenchTop
100bp DNA Ladder
Cat.# G8291
Load 6µl/lane.
8651TA