2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
A. Anti-ACTIVE ® MAPK pAb
NGF-Treated Untreated
B. Anti-ACTIVE ® JNK pAb
Sorbitol-Treated Untreated
C. Anti-ACTIVE ® p38 pAb
Sorbitol-Treated Untreated
Detection of activated MAPK, JNK and p38 in PC12 cells by
immunocytochemistry. PC12 cells were grown to 80% confluence
in RPMI 1640 medium supplemented with 25mM HEPES, 300mg/L
l-glutamine, 10% horse serum, 5% fetal bovine serum and 0.5mM
EGTA. Cells were either untreated or treated with 200ng/ml NGF or 1M
sorbitol as indicated. ICC was performed as described in Promega Technical
Bulletin #TB262. Anti-ACTIVE ® antibodies were used at the following dilutions:
Panel A. MAPK, 1:500; Panel B. JNK, 1:1,000; Panel C. p38, 1:500.
Protocols developed and performed at Promega.
68
2870TA02_0A
Product Size Cat.# Price ($)
Anti-ERK 1/2 pAb, Rabbit 40 µl V1141 367.00
kDa
98 –
64 –
50 –
36 –
Anti-ACTIVE ®
MAPK pAb
Anti-ERK
1/2 pAb
1 2 1 2
Detection of the specifically phosphorylated form of MAPK in NGFtreated
PC12 cell extracts. Anti-ACTIVE ® MAPK pAb (Cat.# V8031) and
Anti-ERK 1/2 (“pan ERK 1/2”) pAb (Cat.# V1141) detection of ERK 1/2 in
untreated (lanes 1) or NGF-treated (lanes 2) PC12 cell extracts (2µg).
Product Size Cat.# Price ($)
Donkey Anti-Rabbit IgG (H+L), HRP 60 µl V7951 179.00
Protein Kinase Inhibitors and
Activators
Product Size Cat.# Price ($)
MEK Inhibitor U0126 5 mg V1121 293.00
cAMP-Dependent Protein Kinase Peptide Inhibitor 1 mg V5681 338.00
Myristoylated Protein Kinase C Peptide Inhibitor 1 mg V5691 147.00
InCELLect AKAP St-Ht31 Inhibitor Peptide 150 µl V8211 486.00
InCELLect St-Ht31P Control Peptide 150 µl V8221 486.00
Olomoucine (cdc2 Protein Kinase Inhibitor) 0.5 mg V2372 99.00
10 mg V2373 481.00
PD 98059 5 mg V1191 293.00
SB 203580 1 mg V1161 293.00
LY 294002 5 mg V1201 367.00
PMA 5 mg V1171 367.00
4α-PMA 1 mg V1181 222.00
cGMP, 1mM 500 µl V6411 76.00
cAMP, 1mM 500 µl V6421 76.00
For complete and up-to-date product information visit: www.promega.com/catalog
2582TB03_9A