2012 Promega catalogue

Cell Signaling
Product Size Cat.# Price ($)
Anti-ACTIVE ® JNK pAb, Rabbit, (pTPpY) 40 µl V7931 671.00
120 µl V7932 1115.00
Immunocytochemical detection of active JNK enzyme in Drosophila
pupal retina using the Anti-ACTIVE ® JNK pAb. Drosophila pupal retina
at 25% of pupal development were fixed in 3% paraformaldehyde in
PBS. The Anti-ACTIVE ® JNK pAb was diluted 1:100 in PBS containing 10%
fetal bovine serum and 0.2% Triton ® X-100. Samples were incubated with
the primary antibody overnight at 4˚C, washed 3 times (10 minutes each) with
0.2% Triton ® X-100 and then incubated with a goat anti-rabbit Cy ® 3 conjugate
for 2 hours at 4˚C. The results illustrate the presence of dually phosphorylated,
active forms of JNK in discrete structures of the fly retinal ommatidia including
intense staining of the inner cone cells as well as the mechanosensory bristles
and surrounding pigment cells. The pattern of staining (which was distinct from
results obtained with an antibody for active p38) and the absence of staining
in control experiments (data not shown) support the high specificity of the Anti-
ACTIVE ® JNK pAb. Image kindly provided by David T. Miller and Ross Cagan,
Department of Molecular Biology and Pharmacology, Washington University
School of Medicine, St. Louis, Missouri.
For complete and up-to-date product information visit: www.promega.com/catalog
1825TA07_7A
Product Size Cat.# Price ($)
Anti-ACTIVE ® MAPK pAb, Rabbit, (pTEpY) 40 µl V8031 671.00
Anti-ACTIVE ® p38 pAb, Rabbit, (pTGpY) 100 µl V1211 671.00
A.
B.
Anti-ACTIVE
NGF
kDa – +
® MAPK
98 –
64 –
50 –
36 –
– ERK1
– ERK2
– JNK2
– JNK1
1 2 3 4 5 6
Anti-MAPK
NGF
kDa – +
98 –
64 –
50 –
36 –
– ERK1
– ERK2
Anti-ACTIVE
Sorbitol
– +
® JNK
Anti-JNK
Sorbitol
– +
– JNK2
– JNK1
Anti-ACTIVE
Sorbitol
– +
® p38
Anti-p38
Sorbitol
– +
1 2 3 4 5 6
– p38
– p38
Detection of MAPK, JNK and p38 in PC12 cell extracts. Panel A.
Western blot analysis using Anti-ACTIVE ® MAPK, Anti-ACTIVE ® JNK and
Anti-ACTIVE ® p38 polyclonal antibodies to detect activated MAPK, JNK and
p38. Panel B. Western blot analysis using anti-MAPK, anti-JNK and anti-p38
antibodies to detect activated and nonactivated MAPK, JNK and p38 in
untreated or NGF- or sorbitol-treated PC12 cells.
1859TC
67
3
Cell Signaling
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