2012 Promega catalogue

Cell Signaling
Casein Kinase I
Product Size Cat.# Price ($)
Casein Kinase I 100 u V5631 323.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Casein Kinase I (CKI or CK-1) is a ubiquitous and highly
conserved serine/threonine protein kinase found in eukaryotic cells. CKI exists
in multiple forms in mammalian tissue and is present in the nucleus, cytosol,
plasma membrane and microsomes. CKI isolated from most species is a
35–37kDa monomer. In contrast to Casein Kinase II, CKI primarily uses Mg 2+ /
ATP as the phosphate donor and is not sensitive to heparin inhibition.
Storage Conditions: Store at –20°C.
Protocol Part#
Casein Kinase I Product Information 9PIV563
Casein Kinase II
Product Size Cat.# Price ($)
Casein Kinase II
For Research Use Only. Not for Use in Diagnostic Procedures.
100 u V5621 323.00
Description: Casein Kinase II (CKII or CK-2) is a ubiquitous serine/threonine
protein kinase found in eukaryotic cells and is also known as phosvitin kinase,
glycogen synthase 5 kinase, troponin T kinase and casein kinase G. CKII is a
multifunctional protein kinase implicated in a variety of cellular processes and
functions, including mitosis and cellular transformation.
CKII isolated from most species is composed of α and α´ subunits (37–44kDa)
and β subunits (24–28kDa). The holoenzyme exists as an αα´β 2 tetramer. The
α subunit contains the catalytic domain, whereas the β subunits presumably
regulate the catalytic activity of the holoenzyme. Casein Kinase II is purified
from rat liver.
Storage Conditions: Store at –70°C.
Protocol Part#
Casein Kinase II Product Information 9PIV562
EGF Receptor
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Product Size Cat.# Price ($)
EGF Receptor
For Research Use Only. Not for Use in Diagnostic Procedures.
10 u V5551 734.00
Description: Epidermal Growth Factor Receptor (EGF Receptor) is a cellsurface
glycoprotein composed of a single polypeptide chain that binds the
peptide Epidermal Growth Factor (EGF). The EGF Receptor consists of an
extracellular ligand binding domain, a single transmembrane region and a cytoplasmic
intrinsic tyrosine kinase domain. Upon ligand binding, the EGF Receptor
autophosphorylates, activating the tyrosine kinase domain of the EGF Receptor.
EGF Receptor is immunopurified from the A431 cell line following a procedure
detailed by Weber et al.. The purified EGF Receptor does possess tyrosine
kinase activity due to the bound EGF; however, the EGF Receptor has not been
autophosphorylated.
Storage Conditions: Store at –70°C.
Protocol Part#
EGF Receptor Protocol 9PIV555
Protein Kinase C
Product Size Conc. Cat.# Price ($)
Protein Kinase C 1 µg 25 µg/ml V5261 488.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Protein Kinase C is an 82kDa monomeric enzyme consisting of a
C-terminal catalytic domain and a cysteine-rich N-terminal regulatory domain.
The regulatory domain contains the sites for calcium and phospholipid binding
and a pseudosubstrate subdomain, the target for PKC autophosphorylation.
PKC is isolated from rat brain following the procedure of Walton et al. The
purified PKC consists primarily of α, β and γ isoforms with lesser amounts of δ
and ζ isoforms.
Features:
• Highly Pure: PKC is greater than 90% pure as determined by SDS-PAGE.
Storage Conditions: Store at –70°C.
Protocol Part#
Protein Kinase C Product Information 9PIV526
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Cell Signaling
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