2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 60 Kinase Enzyme Systems-continued Product Cat.# Size Kinase Molecular Weight Substrate Other RON Kinase Enzyme System V3921 10µg ADP-Glo Kinase Assay + RON Kinase Enzyme System V8071 1 each RSK2 Kinase Enzyme System V3501 10µg ADP-Glo Kinase Assay + RSK2 Kinase Enzyme System V9651 1 each SGK1 Kinase Enzyme System V2911 10µg ADP-Glo Kinase Assay + SGK1 Kinase Enzyme System V9671 1 each SRC Kinase Enzyme System V2921 10µg ADP-Glo Kinase Assay + SRC Kinase Enzyme System V9741 1 each SYK Kinase Enzyme System V3801 10µg ADP-Glo Kinase Assay + SYK Kinase Enzyme System V8271 1 each TBK1 Kinase Enzyme System V3991 10µg ADP-Glo Kinase Assay + TBK1 Kinase Enzyme System V8291 1 each TGFbR2 Kinase Enzyme System V3931 10µg ADP-Glo Kinase Assay + TGFbR2 Kinase Enzyme System V8301 1 each TRKA Kinase Enzyme System V2931 10µg ADP-Glo Kinase Assay + TRKA Kinase Enzyme System V9761 1 each ULK1 Kinase Enzyme System V3521 10µg ADP-Glo Kinase Assay + ULK1 Kinase Enzyme System V9191 1 each ZAP70 Kinase Enzyme System V3811 10µg ADP-Glo Kinase Assay + ZAP70 Kinase Enzyme System V8311 1 each RON, 10µg (Human, recombinant; amino acids 983-end) RSK2, 10µg (Human, recombinant full-length) SGK1, 10µg (Human, recombinant; amino acids 60-end) SRC, 10µg (Human, recombinant full-length) SYK, 10µg (Human, recombinant full-length) TBK1, 10µg (Human, recombinant full-length) TGFbR2, 10µg (Human, recombinant full-length) TRKA, 10µg (Human, recombinant; amino acids 440-end) ULK1, 10µg (Human, recombinant; amino acids 1–649) ZAP70, 10µg (Human, recombinant full-length) ~71 kDa ~112 kDa Axltide (KKSRGDYMTMQIG); derived from the mouse Insulin receptor substrate 1 (amino acid 979-989). RSK Substrate (KRRRLSSLRA); Derived from human 40S ribosomal protein S6 (amino acid 230-239). Reaction Buffer; DTT Reaction Buffer; DTT ~73 kDa Akt (PKB) substrate (CKRPRAASFAE) Reaction Buffer; DTT ~83 kDa SRC substrate (KVEKIGEGTYGVVYKamide); derived from human p34cdc2 (amino acid 6-20). Reaction Buffer; DTT; MnCl 2 ~100kDa Poly (4:1 Glu, Tyr) Peptide Reaction Buffer; DTT ~105kDa ~68kDa Native Swine Myelin Basic Protein (MBP) Native Swine Myelin Basic Protein (MBP) Reaction Buffer; DTT Reaction Buffer; DTT ~66 kDa Poly (4:1 Glu, Tyr) Peptide Reaction Buffer; DTT ~125kDa Native Swine Myelin Basic Protein (MBP) ~96 kDa Poly (4:1 Glu, Tyr) Peptide Reaction Buffer; DTT Reaction Buffer; DTT; MnCl 2 9526LE 9526LE For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling Kinase-Glo ® Kinase-Glo Luminescent Kinase Assays ® Luminescent Kinase Assays Product Size Cat.# Price ($) Kinase-Glo ® Luminescent Kinase Assay 10 ml V6711 153.00 10 × 10 ml V6712 690.00 100 ml V6713 592.00 10 × 100 ml V6714 Pls. Enq. Kinase-Glo ® Max Luminescent Kinase Assay 10 ml V6071 178.00 10 × 10 ml V6072 782.00 100 ml V6073 697.00 10 × 100 ml V6074 Pls. Enq. Kinase-Glo ® Product Size Cat.# Price ($) Kinase-Glo Plus Luminescent Kinase Assay 10 ml V3771 168.00 10 × 10 ml V3772 753.00 100 ml V3773 653.00 10 × 100 ml V3774 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. ® Luminescent Kinase Assay 10 ml V6711 153.00 10 × 10 ml V6712 690.00 100 ml V6713 592.00 10 × 100 ml V6714 Pls. Enq. Kinase-Glo ® Max Luminescent Kinase Assay 10 ml V6071 178.00 10 × 10 ml V6072 782.00 100 ml V6073 697.00 10 × 100 ml V6074 Pls. Enq. Kinase-Glo ® Plus Luminescent Kinase Assay 10 ml V3771 168.00 10 × 10 ml V3772 753.00 100 ml V3773 653.00 10 × 100 ml V3774 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Kinase-Glo ® Luminescent Kinase Assays are homogeneous nonradioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction. The assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS). The assay procedure involves a single reagent addition directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity. The Kinase-Glo ® Assays generate a “glow-type” luminescent signal produced using a patented stabilized luciferase (Ultra-Glo Luciferase) coupled with a proprietary buffer system. When assayed in the presence of kinase reaction buffers, such as the reaction buffer for PKA, the half-life of the luminescent output is greater than five hours, eliminating the need for luminometers with injectors and allowing for batch plate processing. The assay produces excellent Z´-factor values of greater than 0.7 in 96- and 384-well formats, easily detects known kinase inhibitors and provides IC50 values comparable to those reported in the literature. The Kinase-Glo ® Assay systems are differentiated by their linear response to ATP (see figure below). The original Kinase-Glo ® Assay is linear to 10μM ATP, while Kinase-Glo ® Plus Assay is linear to 100μM ATP. The newest assay, Kinase-Glo ® Max, is linear to 500μM ATP, making it well suited for use with kinases with high Km for ATP as well as for screening for kinase inhibitors that do not compete at the ATP binding site. Features: • Assay a Variety of Kinases: Use with a wide range of kinases (including lipid, sugar and alcohol kinases) and substrates (peptides, proteins, lipids, sugars and alcohols). • Obtain Reliable Results: Less interference from library compounds. Z´-factor values greater than 0.7 in either 96- or 384-well plate formats. • Simplify Your Assay: Homogeneous—everything is performed in a single well. • Non-Radioactive: No radioactive waste disposal and safety issues. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ • Screen for Non-ATP Binding Site Inhibitors: Use ATP concentrations as high as 500μM (Kinase-Glo ® Description: The Kinase-Glo Max Assay). .Storage Conditions: Store at –20°C. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes. These fluctuations can greatly alter product stability. ® Luminescent Kinase Assays are homogeneous nonradioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction. The assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS). The assay procedure involves a single reagent addition directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity. The Kinase-Glo ® Assays generate a “glow-type” luminescent signal produced using a patented stabilized luciferase (Ultra-Glo Luciferase) coupled with a proprietary buffer system. When assayed in the presence of kinase reaction buffers, such as the reaction buffer for PKA, the half-life of the luminescent output is greater than five hours, eliminating the need for luminometers with injectors and allowing for batch plate processing. The assay produces excellent Z´-factor values of greater than 0.7 in 96- and 384-well formats, easily detects known kinase inhibitors and provides IC50 values comparable to those reported in the literature. The Kinase-Glo ® Assay systems are differentiated by their linear response to ATP (see figure below). The original Kinase-Glo ® Assay is linear to 10μM ATP, while Kinase-Glo ® Plus Assay is linear to 100μM ATP. The newest assay, Kinase-Glo ® Max, is linear to 500μM ATP, making it well suited for use with kinases with high Km for ATP as well as for screening for kinase inhibitors that do not compete at the ATP binding site. Features: • Assay a Variety of Kinases: Use with a wide range of kinases (including lipid, sugar and alcohol kinases) and substrates (peptides, proteins, lipids, sugars and alcohols). • Obtain Reliable Results: Less interference from library compounds. Z´-factor values greater than 0.7 in either 96- or 384-well plate formats. • Simplify Your Assay: Homogeneous—everything is performed in a single well. • Non-Radioactive: No radioactive waste disposal and safety issues. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ • Screen for Non-ATP Binding Site Inhibitors: Use ATP concentrations as high as 500μM (Kinase-Glo ® Max Assay). .Storage Conditions: Store at –20°C. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes. These fluctuations can greatly alter product stability. Protocol Part# Kinase-Glo ® Protocol Part# Kinase-Glo Luminescent Kinase Assay Platform Technical Bulletin TB372 ® Luminescent Kinase Assay Platform Technical Bulletin TB372 A. 3.0 × 108 A. 3.0 × 108 For complete and up-to-date product information visit: www.promega.com/catalog Luminescence (RLU) Luminescence (RLU) 2.5 × 108 2.5 × 108 2.0 × 108 2.0 × 108 1.5 × 108 1.5 × 108 1.0 × 108 1.0 × 108 0.5 × 108 0.5 × 108 0 0 r2 r =0.9993 2 =0.9993 20 40 60 ATP (µM) 80 100 120 B. y = 280837x + 2 × 10 ATP (µM) 16 r2 2 × 10 = 0.9971 0 7 4 × 107 6 × 107 8 × 107 1 × 108 1.2 × 108 1.4 × 108 1.6 × 108 B. y = 280837x + 2 × 10 0 100 200 300 400 500 600 ATP (µM) 16 r2 2 × 10 = 0.9971 0 7 4 × 107 6 × 107 8 × 107 1 × 108 1.2 × 108 1.4 × 108 1.6 × 108 0 100 200 300 400 500 600 Luminescent output correlates with amount of ATP. A direct relationship exists between the luminescence measured with the Kinase-Glo ® Assay systems and the amount of ATP in the reaction. Panel A. Kinase-Glo ® Plus Assay. Panel B. Kinase-Glo ® Max Assay. The Kinase-Glo ® Luminescent output correlates with amount of ATP. A direct relationship exists between the luminescence measured with the Kinase-Glo Assay is linear to 10μM (data not shown). ® Assay systems and the amount of ATP in the reaction. Panel A. Kinase-Glo ® Plus Assay. Panel B. Kinase-Glo ® Max Assay. The Kinase-Glo ® Assay is linear to 10μM (data not shown). ProFluor ® ProFluor PKA Assay ® PKA Assay Product Size Cat.# Price ($) ProFluor ® Product Size Cat.# Price ($) ProFluor PKA Assay 4 plate V1240 1185.00 For Research Use Only. Not for Use in Diagnostic Procedures. 8 plate V1241 2040.00 ® PKA Assay 4 plate V1240 1185.00 For Research Use Only. Not for Use in Diagnostic Procedures. 8 plate V1241 2040.00 Description: The ProFluor ® Description: The ProFluor PKA Assay measures protein kinase A activity using purified kinase in a multiwell plate format and involves “add-mix-read” steps only—ideal for high-throughput applications. The assay begins with a standard kinase reaction performed with a provided PKA bisamide rhodamine 110 peptide substrate. Following the kinase reaction, a termination buffer containing a protease reagent is added, which simultaneously stops the kinase reaction and removes amino acids specifically from the nonphosphorylated PKA substrate, liberating highly fluorescent rhodamine 110. Phosphorylated PKA substrate, however, is resistant to digestion by the protease reagent and remains nonfluorescent. Thus, fluorescence intensity measured in this assay is inversely correlated with kinase activity. The assay produces excellent Z´-factor values (>0.8) in either 96- or 384-well plate formats and easily distinguishes known PKA inhibitors from other compounds. Features: • Achieve Highly Predictive Results: Robust Z´ values greater than 0.7 in either 96- or 384-well plate formats. • Observe Minimal Test Compound Interference: Fluorescent signal from Rhodamine 110 is much stronger than fluorescent signals given off by test compounds. • Homogeneous: Add-mix-read format reduces the number of steps. • Non-Radioactive: No radioactive waste disposal and safety issues. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store the entire system at –20°C. Protect the PKA R110 Substrate from light. For best results, make solutions fresh and use immediately. System components should be thawed on ice and returned to –20°C as soon as possible. The PKA R110 Substrate is provided in 100% DMSO and therefore requires thawing at room temperature. ® PKA Assay measures protein kinase A activity using purified kinase in a multiwell plate format and involves “add-mix-read” steps only—ideal for high-throughput applications. The assay begins with a standard kinase reaction performed with a provided PKA bisamide rhodamine 110 peptide substrate. Following the kinase reaction, a termination buffer containing a protease reagent is added, which simultaneously stops the kinase reaction and removes amino acids specifically from the nonphosphorylated PKA substrate, liberating highly fluorescent rhodamine 110. Phosphorylated PKA substrate, however, is resistant to digestion by the protease reagent and remains nonfluorescent. Thus, fluorescence intensity measured in this assay is inversely correlated with kinase activity. The assay produces excellent Z´-factor values (>0.8) in either 96- or 384-well plate formats and easily distinguishes known PKA inhibitors from other compounds. See figure on next page. Features: • Achieve Highly Predictive Results: Robust Z´ values greater than 0.7 in either 96- or 384-well plate formats. • Observe Minimal Test Compound Interference: Fluorescent signal from Rhodamine 110 is much stronger than fluorescent signals given off by test compounds. • Homogeneous: Add-mix-read format reduces the number of steps. • Non-Radioactive: No radioactive waste disposal and safety issues. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store the entire system at –20°C. Protect the PKA R110 Substrate from light. For best results, make solutions fresh and use immediately. System components should be thawed on ice and returned to –20°C as soon as possible. The PKA R110 Substrate is provided in 100% DMSO and therefore requires thawing at room temperature. Protocol Part# ProFluor ® Protocol Part# ProFluor PKA Assay Technical Bulletin TB315 ® PKA Assay Technical Bulletin TB315 6979MB 61 3 Cell Signaling Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
Page 13 and 14: Cell Signaling Sephacryl ® S-400 P
Page 15 and 16: Cell Signaling Tris Base, Molecular
Page 17 and 18: Cell Signaling Unmethylated Lambda
Page 19 and 20: Cell Signaling Promega Barrier Tips
Page 21 and 22: Cell Signaling Product Size Cat.# P
Page 23 and 24: Cell Signaling 2 Cell Health Assays
Page 25 and 26: Cell Signaling A. B. Luminescence/p
Page 27 and 28: Cell Signaling Luminogenic Enzyme S
Page 29 and 30: Cell Signaling Apoptosis
Page 31 and 32: Cell Signaling Caspase-Glo ® 2 Ass
Page 33 and 34: Cell Signaling Caspase-Glo ® 9 Ass
Page 35 and 36: Cell Signaling CaspACE Assay System
Page 37 and 38: Cell Signaling Anti-ACTIVE ® Caspa
Page 39 and 40: Cell Signaling BacTiter-Glo Microbi
Page 41 and 42: Cell Signaling CellTiter 96 ® Cell
Page 43 and 44: Cell Signaling MultiTox-Fluor Multi
Page 45 and 46: Cell Signaling CytoTox-Glo Cytotoxi
Page 47 and 48: Cell Signaling CytoTox 96 ® Non-Ra
Page 49 and 50: Cell Signaling GSH-Glo Glutathione
Page 51 and 52: Cell Signaling 3 Cell Signaling For
Page 53 and 54: Cell Signaling cAMP-Glo Max Assay P
Page 55 and 56: Cell Signaling Growth Factors Epide
Page 57 and 58: Cell Signaling SIRT-Glo Assays and
Page 59 and 60: Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62: Cell Signaling Kinase Enzyme System
Page 63: Cell Signaling Kinase Enzyme System
Page 67 and 68: Cell Signaling SignaTECT ® Protein
Page 69 and 70: Cell Signaling Casein Kinase I Prod
Page 71 and 72: Cell Signaling Product Size Cat.# P
Page 73 and 74: Cell Signaling Protein Kinase Subst
Page 75 and 76: Cell Signaling For complete and up-
Page 77 and 78: Cell Signaling DNA Step Ladders Pro
Page 79 and 80: Cell Signaling Conventional DNA Mar
Page 81 and 82: Cell Signaling ProMega-Markers ® L
Page 83 and 84: Cell Signaling Restriction Enzymes
Page 85 and 86: Cell Signaling BamHI Product Size C
Page 87 and 88: Cell Signaling EcoRI Product Size C
Page 89 and 90: Cell Signaling NciI Product Size Co
Page 91 and 92: Cell Signaling SpeI Product Size Co
Page 93 and 94: Cell Signaling TSAP Thermosensitive
Page 95 and 96: Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98: Cell Signaling Kinases T4 Polynucle
Page 99 and 100: Cell Signaling S1 Nuclease Product
Page 101 and 102: Cell Signaling Percent Inhibition o
Page 103 and 104: Cell Signaling Untagged Flexi ® Ma
Page 105 and 106: Cell Signaling pGEM ® -5Zf(+) Vect
Page 107 and 108: Cell Signaling pSP64 Poly(A) Vector
Page 109 and 110: Cell Signaling For complete and up-
Page 111 and 112: Cell Signaling x-tracta Gel Extract
Page 113 and 114: Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Page 185 and 186:
Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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2012 Promega catalogue

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