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2012 Promega catalogue

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Life<br />

Science<br />

Catalog<br />

<strong>2012</strong><br />

Worldwide Contact List<br />

Section<br />

Contents<br />

Table of<br />

Contents<br />

Cell Signaling<br />

Biochemical Buffers and Reagents<br />

2<br />

4-CORE ® Buffer Pack<br />

Product Size Cat.# Price ($)<br />

4-CORE ® Buffer Pack (Buffers A, B, C and D), 1ml<br />

each 4ml R9921 22.00<br />

For Laboratory Use.<br />

For additional information see page 88.<br />

5M Sodium Chloride, Molecular Biology<br />

Grade<br />

Product Size Conc. Cat.# Price ($)<br />

5M Sodium Chloride, Molecular Biology<br />

Grade 1L 5M V4221 65.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

Description: 5M Sodium Chloride is commonly used in many molecular<br />

biology and forensic applications.<br />

Form: Clear, colorless liquid.<br />

Composition: 292.2g/L NaCl in deionized water.<br />

Properties:<br />

• pH at 25°C (1M): 5.0–8.0.<br />

• A 260 at 5M: ≤0.02.<br />

• A 280 at 5M: ≤0.01.<br />

• Conductivity at 25°C (0.05M): 5,000–7,000μSm.<br />

Features:<br />

• Quality Tested: Each lot of NaCl is tested and certified to be free of<br />

DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

Acrylamide, Molecular Grade<br />

Product Size Cat.# Price ($)<br />

Acrylamide, Molecular Grade 100 g V3111 90.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

500 g V3115 210.00<br />

Description: Acrylamide, Molecular Grade, is used for the electrophoretic<br />

separation of nucleic acids and proteins. Very small DNA fragments, such as<br />

those generated by sequencing reactions, can be resolved by polyacrylamide<br />

gel electrophoresis. Proteins can be separated by a variety of techniques,<br />

including denaturing gel electrophoresis using SDS or urea, isoelectric<br />

focusing and native gel electrophoresis in a wide variety of buffers.<br />

Formula Weight: 71.08.<br />

Form: White, free-flowing crystals.<br />

Properties:<br />

• Purity: ≥99.9%.<br />

• Melting Point: 84–86°C.<br />

• Free Acrylic Acid: 1,000bp)<br />

Product<br />

Agarose, LMP, Preparative Grade for Large<br />

Size Cat.# Price ($)<br />

Fragments (>1,000bp)<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

25g V2831 377.00<br />

Description: Agarose, LMP, Preparative Grade for Large Fragments<br />

(>1,000bp), is a premium agarose used for isolating DNA fragments larger<br />

than 1,000bp. Each lot is tested and certified for the following applications:<br />

1) restriction digestion, 2) ligation and transformation, and 3) random prime<br />

labeling. LMP = low melting point (i.e., ≤65°C).<br />

Form: White powder.<br />

Properties:<br />

• Gelling Point (1.5%): 26–30°C.<br />

• Melting Point (1.5%): ≤65°C.<br />

• Sulfate: ≤0.10%.<br />

• EEO (–mr): ≤0.10.<br />

• Moisture: ≤10%.<br />

• Gel Strength (1%): ≥200g/cm 2 .<br />

Features:<br />

• Quality Tested: Each lot of Preparative Grade LMP Agarose is tested and<br />

certified to be free of DNase and RNase activity.<br />

Storage Conditions: Store at 22–25°C.<br />

For complete and up-to-date product information visit: www.promega.com/catalog

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