2012 Promega catalogue

Cell Signaling
Growth Factors
Epidermal Growth Factor, Human,
Recombinant
Product Size Cat.# Price ($)
rhEGF 100 µg G5021 358.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Epidermal Growth Factor, Human, Recombinant (rhEGF) is a
6.2kDa protein that is mitogenic for a variety of mammalian cell types. rhEGF is
produced from recominbant DNA expressed in E. coli.
Activity: rhEGF exhibits an ED 50 value below 0.2ng/ml in the serum-free
BALB/3T3 bioassay using the CellTiter 96 ® Non-Radioactive Cell Proliferation
Assay.
Storage Conditions: Store lyophilized product at –20°C. Rehydrated rhEGF is
stable for 3 months at –20°C. Avoid repeated freeze-thaw cycles.
Protocol Part#
Epidermal Growth Factor, Human, Recombinant (rhEGF) and Murine
Receptor Grade Protocol
9PIG502
Human Brain Derived Neurotrophic Factor
(BDNF)
Product Size Cat.# Price ($)
rhBDNF
For Research Use Only. Not for Use in Diagnostic Procedures.
5 µg G1491 631.00
Description: Brain Derived Neurotrophic Factor, Human, Recombinant
(rhBDNF) is a 27kDa homodimer originally shown to promote the outgrowth of
spinal sensory neurons. rhBDNF is produced from recombinant DNA expressed
in E. coli.
Storage Conditions: Stable for 6 months when stored desiccated at –20°C.
Store reconstituted product in working aliquots at –20°C, where it is stable for
3 months. Avoid multiple freeze-thaw cycles.
Protocol Part#
Brain Derived Neurotrophic Factor, Human, Recombinant 9PIG149
Human Glial Cell-Line Derived Neurotrophic
Factor (GDNF)
Product Size Cat.# Price ($)
rhGDNF
For Research Use Only. Not for Use in Diagnostic Procedures.
5 µg G2781 631.00
Description: Human Glial Cell-Lined Derived Neurotrophic Factor (rhGDNF)
is a 30kDa homodimer consisting of two disulfide-linked, 134 amino acid
subunits. GDNF promotes dopamine uptake and survival of midbrain neurons
and is also a survival factor for developing motor neurons, purified rat embryo
spinal neurons and nodose sensory neurons. rhGDNF is produced from recombinant
DNA expressed in E. coli.
Storage Conditions: Stable for 6 months when stored desiccated at –20°C.
Store reconstituted product in working aliquots at –20°C, where it is stable for
3 months from date of purchase. Avoid multiple freeze-thaw cycles.
Protocol Part#
Glial Cell Line-Derived Neurotrophic Factor, Human, Recombinant
Protocol
9PIG278
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Nerve Growth Factor, 2.5S, Murine
Product Size Cat.# Price ($)
mNGF, 2.5S
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µg G5141 502.00
Description: Murine 2.5S Nerve Growth Factor (2.5S mNGF) mediates
phosphorylation of specific intracellular proteins. Target cells of this molecule
include sympathetic and sensory neurons and derivatives of nerve cells such
as PC12 cells. 2.5S mNGF is a 26kDa protein composed of two identical 118
amino acid chains. Murine 2.5S Nerve Growth Factor is purified from male
mouse submaxillary glands by the method of Bocchini and Angeletii.
Activity: 2.5S mNGF exhibits an ED 50 value below 2ng/ml using a PC-12
serum-free survival assay.
Storage Conditions: Store lyophilized Murine 2.5S NGF desiccated at –20°C.
Store reconstituted Murine 2.5S NGF in working aliquots at –20°C.
Protocol Part#
Nerve Growth Factor, 2.5S, Murine Protocol 9PIG514
rhFGF, Basic
Product Size Cat.# Price ($)
rhFGF, Basic
For Research Use Only. Not for Use in Diagnostic Procedures.
25 µg G5071 639.00
Description: Fibroblast Growth Factor, Basic, Human, Recombinant (rhFGF,
Basic), is a 17.5kDa polypeptide containing 154 amino acids. It induces proliferation
of multiple cell types in vitro and shows potent angiogenic activity in
vivo. rhFGF, Basic, is produced from recombinant DNA expressed in E. coli.
Protocol Part#
Fibroblast Growth Factor, Basic, Human, Recombinant Protocol 9PIG507
rhIGF-I
Product Size Cat.# Price ($)
rhIGF-I
For Research Use Only. Not for Use in Diagnostic Procedures.
25 µg G5111 792.00
Description: Insulin-Like Growth Factor-I, Human, Recombinant (rhIGF-I), is a
7.6kDa protein containing 70 amino acid residues. It stimulates the proliferation
of a wide range of cell types. rhIGF-I is a highly purified, biologically active,
recombinant molecule, produced in E. coli.
Protocol Part#
Insulin-like Growth Factor I, Human, Recombinant Protocol 9PIG511
rhTNF-α
Product Size Cat.# Price ($)
rhTNFα
For Research Use Only. Not for Use in Diagnostic Procedures.
10 µg G5241 545.00
Description: Tumor Necrosis Factor-α, Human, Recombinant (rhTNF-α),
is a pleiotropic cytokine produced predominantly by activated monocytes/
macrophages that can induce apoptosis, cytolysis or cytostasis of tumor cells,
activation of polymorphonuclear leukocytes, antiviral activity and induction
of IL-1 or colony-stimulating factor expression. rhTNF-α is a 17kDa protein
containing 157 amino acid residues that is produced from a recombinant DNA
expressed in E. coli.
Protocol Part#
Tumor Necrosis Factor alpha, Human, Recombinant Protocol 9PIG524
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Cell Signaling
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