2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling Mitochondrial Function Assay 46 Mitochondrial Toxicity Assay Product Size Cat.# Price ($) Mitochondrial ToxGlo Assay 10 ml G8000 286.00 For Research Use Only. Not for Use in Diagnostic Procedures. 100 ml G8001 1678.00 Description: The Mitochondrial ToxGlo Assay is a cell-based assay method that employs sequential addition, multiplexed assay chemistry for predicting potential mitochondrial dysfunction as a result of xenobiotic exposure. The assay is based on the differential measurement of biomarkers associated with changes in cell membrane integrity and cellular ATP levels relative to vehicle-treated control cells during short exposure periods. Cell membrane integrity is first assessed by measuring the presence or abscence of a distinct protease activity associated with necrosis using a fluorogenic peptide substrate (bis-AAF-R110) to measure “dead cell protease activity”. The bis-AAF-R110 Substrate cannot cross the intact membrane of live cells and therefore gives no signal with viable cells. Next, ATP is measured by adding an ATP detection reagent, resulting in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The two sets of data can be combined to produce profiles representative of mitochondrial dysfunction or non-mitochondrial related cytotoxic mechanisms. Mammalian cells generate ATP by mitochondrial (oxidative phosphorylation) and nonmitochondrial (glycolysis) methods. To achieve optimal mitochondrial responsiveness, replacing glucose-supplemented medium with galactosecontaining medium may increase cellular oxygen consumption and augment mitochondrial susceptibility to mitotoxicants. A. 150 ATP Cytotoxicity B. Percent Vehicle Control 100 50 0 100 50 0 –7 –6 –5 Log [imipramine], M C. D. 150 Percent Vehicle Control –7 –6 Log [CCCP], M –5 Features: • Distinguish Primary Mitochondrial Dysfunction from Secondary Cytotoxic Events: Cell-based, multiplexed method measures ATP in conjunction with a membrane integrity biomarker to distinguish primary mitochondrial dysfunction from secondary cytotoxic events. • Predictive for Mitochondrial Toxicities: Produces profiles that are consistent with mitochondrial toxicity and discernible from other nonmitotoxic mechanisms of cell death. • Easy to Implement: The assay uses a simple sequential “add-mix-read” format to assess toxicity in the sample well. • Fast: Quickly assess potential mitochondrial liabilities in under an hour. • Cost-Effective: Assays are performed directly in cell culture plates using standard multimode detection instrumentation. • Flexible and Easily Automated: The volume of reagent addition can be scaled to meet throughput needs; the assay is amenable to automation in 96- and 384-well plates. Storage Conditions: Store the Mitochondrial Tox-Glo Assay components at –20°C. Protocol Part# Mitochondrial ToxGlo Assay Technical Manual TM357 Percent Vehicle Control Percent Vehicle Control 250 200 150 100 50 0 600 500 400 300 200 100 –6 –5 Log [digitonin], g/L –4 0 –7 –6 –5 –4 Log [antimycin], g/L Representative profiles of mitochondrial toxicity with the Mitochondrial ToxGlo Assay. K562 cells were plated at 10,000 cells/well in 96-well plates and treated with serial dilutions of compounds resuspended in glucose-free (galactose-supplemented) RPMI 1640 medium for 2 hours. Panel A shows no changes in ATP or membrane integrity (MI), which indicates that the compound is not a mitochondrial toxin. Panel B. The reduction in ATP with commensurate MI changes indicate that the compound is not a mitochondrial toxin; instead primary necrosis is taking place. Panel C. The reduction in ATP with no changes in MI indicates that the compound is a mitochondrial toxin. Panel D. The reduction in ATP with discordant changes in MI indicate that the compound is a mitochondrial toxin. For complete and up-to-date product information visit: www.promega.com/catalog 9955MA
Cell Signaling 3 Cell Signaling For complete and up-to-date product information visit: www.promega.com/catalog GPCR Assays 48 Growth Factors 51 Histone Deacetylase Assays 52 Protein Kinase Assays 54 Protein Kinases 64 Cell Signaling Antibodies 66 Protein Kinase Inhibitors and Activators Protein Phosphatases and Phosphatase Assays 68 69 47 3 Cell Signaling Section Contents Table of Contents
Page 1 and 2: Life Science CATALOG 2012
Page 3 and 4: Cell Signaling Table of Contents Pr
Page 5 and 6: Cell Signaling For complete and up-
Page 7 and 8: Cell Signaling Agarose, LMP, Prepar
Page 9 and 10: Cell Signaling Blue/Orange Loading
Page 11 and 12: Cell Signaling Glycine, Molecular B
Page 13 and 14: Cell Signaling Sephacryl ® S-400 P
Page 15 and 16: Cell Signaling Tris Base, Molecular
Page 17 and 18: Cell Signaling Unmethylated Lambda
Page 19 and 20: Cell Signaling Promega Barrier Tips
Page 21 and 22: Cell Signaling Product Size Cat.# P
Page 23 and 24: Cell Signaling 2 Cell Health Assays
Page 25 and 26: Cell Signaling A. B. Luminescence/p
Page 27 and 28: Cell Signaling Luminogenic Enzyme S
Page 29 and 30: Cell Signaling Apoptosis
Page 31 and 32: Cell Signaling Caspase-Glo ® 2 Ass
Page 33 and 34: Cell Signaling Caspase-Glo ® 9 Ass
Page 35 and 36: Cell Signaling CaspACE Assay System
Page 37 and 38: Cell Signaling Anti-ACTIVE ® Caspa
Page 39 and 40: Cell Signaling BacTiter-Glo Microbi
Page 41 and 42: Cell Signaling CellTiter 96 ® Cell
Page 43 and 44: Cell Signaling MultiTox-Fluor Multi
Page 45 and 46: Cell Signaling CytoTox-Glo Cytotoxi
Page 47 and 48: Cell Signaling CytoTox 96 ® Non-Ra
Page 49: Cell Signaling GSH-Glo Glutathione
Page 53 and 54: Cell Signaling cAMP-Glo Max Assay P
Page 55 and 56: Cell Signaling Growth Factors Epide
Page 57 and 58: Cell Signaling SIRT-Glo Assays and
Page 59 and 60: Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62: Cell Signaling Kinase Enzyme System
Page 63 and 64: Cell Signaling Kinase Enzyme System
Page 65 and 66: Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68: Cell Signaling SignaTECT ® Protein
Page 69 and 70: Cell Signaling Casein Kinase I Prod
Page 71 and 72: Cell Signaling Product Size Cat.# P
Page 73 and 74: Cell Signaling Protein Kinase Subst
Page 75 and 76: Cell Signaling For complete and up-
Page 77 and 78: Cell Signaling DNA Step Ladders Pro
Page 79 and 80: Cell Signaling Conventional DNA Mar
Page 81 and 82: Cell Signaling ProMega-Markers ® L
Page 83 and 84: Cell Signaling Restriction Enzymes
Page 85 and 86: Cell Signaling BamHI Product Size C
Page 87 and 88: Cell Signaling EcoRI Product Size C
Page 89 and 90: Cell Signaling NciI Product Size Co
Page 91 and 92: Cell Signaling SpeI Product Size Co
Page 93 and 94: Cell Signaling TSAP Thermosensitive
Page 95 and 96: Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98: Cell Signaling Kinases T4 Polynucle
Page 99 and 100: Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling For complete and up-
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Page 185 and 186:
Cell Signaling Microbial Detection
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10 Instruments For complete and up-
Page 189 and 190:
GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
Page 271 and 272:
Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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2012 Promega catalogue

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