2012 Promega catalogue

Cell Signaling
CytoTox 96 ® Non-Radioactive Cytotoxicity
Assay
Product Size Cat.# Price ($)
CytoTox 96 ® Non-Radioactive Cytotoxicity
Assay 1,000 assays G1780 429.00
For Laboratory Use.
Description: The CytoTox 96 ® Non-Radioactive Cytotoxicity Assay is a
colorimetric alternative to radioactive cytotoxicity assays. The CytoTox 96 ®
Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic
enzyme that is released upon cell lysis, in much the same way as [ 51 Cr] is
released in radioactive assays. Released LDH in cell culture supernatants
is measured with a 30-minute coupled enzymatic assay that results in the
conversion of a tetrazolium salt (INT) into a red formazan product. The amount
of color formed is proportional to the number of lysed cells. Visible wavelength
absorbance data are collected using a standard 96-well plate reader. The assay
can be used to measure membrane integrity for cell-mediated cytotoxicity
assays in which a target cell is lysed by an effector cell, or to measure lysis of
target cells by bacteria, viruses, proteins, chemicals, etc.
Features:
• Non-Radioactive: Requires no radioactive waste disposal or [ 51 Cr].
• Save Time: Eliminates labeling of target cells prior to experiment.
• Use Standard Equipment: Collect absorbance (visible wavelength) data
with a standard 96-well plate reader.
• Adapt to Your Needs: Used for a variety of applications including
measurement of: 1) cell-mediated cytotoxicity; 2) chemical-mediated
cytotoxicity; and 3) total cell number.
• Gain Sensitivity: Can reveal early, low-level damage to cell membranes
that is often missed with other methodologies.
Storage Conditions: Store Substrate Mix and Assay Buffer at –20°C. Store
LDH Positive Control, Lysis Solution (10X) and Stop Solution at 4°C.
Protocol Part#
CytoTox ® 96 Non-Radioactive Cytotoxicity Assay Technical Bulletin TB163
For complete and up-to-date product information visit: www.promega.com/catalog
Griess Reagent System
Product Size Cat.# Price ($)
Griess Reagent System 1,000 assays G2930 231.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Griess Reagent System measures nitrite (NO 2 – ), which
is one of two primary stable and nonvolatile breakdown products of nitric
oxide (NO). Nitric oxide is an important physiological messenger and effector
molecule in many biological systems, including immunological, neuronal and
cardiovascular tissues. This assay relies on a diazotization reaction that was
originally described by Griess in 1879. Through the years, many modifications
to the original reaction have been described.
The Griess Reagent System is based on a chemical reaction that uses sulfanilamide
and N-1-naphthylethylenediamine dihydrochloride (NED) under acidic
(phosphoric acid) conditions. This system detects NO 2 – in a variety of biological
and experimental liquid matrices such as plasma, serum, urine and tissue
culture medium. The nitrite sensitivity is dependent on the matrix. The limit of
detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized, distilled water)
using the protocol described in Technical Bulletin #TB229.
Storage Conditions: Store at 4°C. Keep all solutions in their original lightprotective
plastic bottles.
Protocol Part#
Griess Reagent System Technical Bulletin TB229
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Cell Health Assays
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