2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
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Table of
Contents
Cell Signaling
42
CytoTox-Fluor Cytotoxicity Assay
Product Size Cat.# Price ($)
CytoTox-Fluor Cytotoxicity Assay 10 ml G9260 201.00
For Laboratory Use.
5 × 10 ml G9261 745.00
2 × 50 ml G9262 1115.00
Description: The CytoTox-Fluor Cytotoxicity Assay measures the relative
number of dead cells in cell populations. The assay uses a fluorogenic peptide
substrate (bis-alanyl-alanyl-phenylanlanyl-rhodamine 110; bis-AAF-R110)
to measure “dead-cell protease activity,” which has been released from cells
that have lost membrane integrity. The bis-AAF-R110 substrate cannot cross
the intact membrane of live cells and therefore gives no signal from live cells.
The assay is designed to accommodate downstream multiplexing with several
Promega luminescent assays or spectrally distinct fluorescent assay methods,
such as assays to measure caspase activation, reporter gene expression or
orthogonal measures of viability.
Features:
• Measure the Relative Number of Dead Cells in Culture: Homogeneous,
“add-mix-measure” protocol eliminates parallel plate processing
and reduces cell culture costs.
• Get More Data from Every Well: Multiplex the CytoTox-Fluor Assay
with several Promega luminescent cell-based assays.
• Normalize Downstream Multiplex Data for Cytotoxicity: Data
normalization for dead-cell number makes results more comparable wellto-well,
plate-to-plate, day-to-day.
• Reduce Assay Variability: The homogeneous “add-mix-measure”
protocol avoids the cumulative error associated with multistep protocols.
Storage Conditions: Store at –20°C.
Protocol Part#
CytoTox-Fluor Cytotoxicity Assay TB350
CytoTox-ONE Homogeneous Membrane
Integrity Assay
Product Size Cat.# Price ($)
CytoTox-ONE Homogeneous
200–800 assays G7890 196.00
Membrane Integrity Assay
CytoTox-ONE Homogeneous
1,000–4,000 assays G7891 741.00
Membrane Integrity Assay, HTP 1,000–4,000 assays G7892 741.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The CytoTox-ONE Homogeneous Membrane Integrity Assay
is a fluorometric method for estimating the number of nonviable cells present
in multiwell plates. The CytoTox-ONE Assay rapidly measures the release
of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH
released into the culture medium is measured with a 10-minute coupled
enzymatic assay that results in the conversion of resazurin into a fluorescent
resorufin product. The amount of fluorescence produced is proportional to
the number of lysed cells. The CytoTox-ONE Reagent does not damage
normal healthy cells; therefore the reactions to measure released LDH can be
performed directly in assay wells containing a mixed population of viable and
damaged cells.
The CytoTox-ONE Homogeneous Membrane Integrity Assay, HTP offers
convenient, alternative packaging for processing multiple plates. Each bottle
of reagent supplied with the system is sufficient to perform 500 assays in a
96-well format or 2,000 assays in a 384-well format when the recommended
volumes are used.
Features:
• Save Time: Complete the assay in the cell culture plate, without a sample
transfer step; read data in 10 minutes, compared to 30 minutes or more
with classic LDH assays.
• Multiplex This Assay: Perform multiple assays on one sample with other
homogeneous cell-based assays from Promega.
• Adapt Protocol to Your Needs: Using the provided stop solution, assays
can be read over several hours while still maintaining good signal.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C protected from light.
Protocol Part#
CytoTox-ONE Homogeneous Membrane Integrity Assay Technical
Bulletin
TB306
Fluorescence (LDH)
3,000
2,800
2,600
2,400
2,200
2,000 0 200 400 600 800 1,000 0
CytoTox-ONE Assay
Staurosporine (nM)
Caspase-Glo ® 3/7 Assay
Multiplexing the CytoTox-ONE Assay and the Caspase-Glo ® 3/7
Assay. With most in vitro apoptosis assays, LDH release occurs
relatively late during the process. The duration of drug exposure here
was carefully chosen to demonstrate the early stages of cell lysis, while still
retaining caspase activity.
For complete and up-to-date product information visit: www.promega.com/catalog
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Luminescence (caspase)
4745MA