2012 Promega catalogue

Life
Science
Catalog
2012
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Contents
Cell Signaling
38
CellTiter-Blue ® Cell Viability Assay
Product Size Cat.# Price ($)
CellTiter-Blue ® Cell Viability Assay 20 ml G8080 196.00
For Research Use Only. Not for Use in Diagnostic Procedures.
100 ml G8081 516.00
10 × 100 ml G8082 Pls. Enq.
Description: The homogeneous CellTiter-Blue ® Cell Viability Assay provides
a fluorescent method for monitoring cell viability. The assay is based on the
ability of living cells to convert a redox dye (resazurin) into a fluorescent end
product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do
not generate a fluorescent signal. The homogeneous assay procedure involves
adding the single reagent directly to cells cultured in serum-supplemented medium.
After an incubation step, data are recorded using either a plate-reading
fluorometer (preferred) or spectrophotometer.
Features:
• Save Time: The homogeneous, add-incubate-measure format reduces the
number of handling steps.
• Perform More Than One Assay on the Same Sample: The system
can be multiplexed with other assay methods: Apo-ONE ® Homogeneous
Caspase-3/7 Assay or the Caspase-Glo ® Assays for detecting apoptosis.
• Gain Flexibility: The CellTiter-Blue ® Assay delivers excellent Z´ factor
values and offers more flexibility in assay incubation times compared to
other resazurin-based assays.
• Safe: The reagent is generally nontoxic to cells, allowing extended
incubation periods in some situations. Requires no scintillation cocktail,
radioactive waste disposal (unlike [ 3 H]-thymidine incorporation assays) or
hazardous solvents (as required for MTT tetrazolium-based assays).
• Adapt to Your Throughput Needs: The reagent is designed to provide
sufficient volumes for accurate pipetting into 96- or 384-well formats.
Convenient product sizes available for high-throughput screening.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store frozen at –20°C protected from light.
Protocol Part#
CellTiter-Blue ® Cell Viability Assay Technical Bulletin TB317
Fluorescence (560/590nm)
3,000
2,500
2,000
1,500
1,000
500
Apo-ONE ® CellTiter-Blue
Assay
® Assay
0 0
0 40 80120 160
Tamoxifen (µM)
10,000
8,000
6,000
4,000
2,000
Multiplexing cell-based assays. Collecting viability data (CellTiter-
Blue ® Assay) and apoptosis data (Apo-ONE ® Caspase-3/7 Assay) from
the same wells.
Fluorescence (485/527nm)
4128MA05_3A
Cytotoxicity Assays
MultiTox-Glo Multiplex Cytotoxicity Assay
Product Size Cat.# Price ($)
MultiTox-Glo Multiplex Cytotoxicity Assay 10 ml G9270 322.00
5 × 10 ml G9271 1195.00
For Laboratory Use.
2 × 50 ml G9272 1791.00
Description: The MultiTox-Glo Multiplex Cytotoxicity Assay is a sequentialreagent-addition
fluorescent and luminescent assay that measures the relative
number of live and dead cells in cell populations. The MultiTox-Glo Assay sequentially
measures two protease activities; one is a marker of viability, and the
other is a marker of cytotoxicity. The live-cell protease activity is restricted to
intact viable cells and is measured using a fluorogenic, cell-permeant, peptide
substrate (GF-AFC). This substrate enters intact cells, where it is cleaved by the
live cell protease activity to release AFC and generate a fluorescent signal that
is proportional to the number of viable cells. The live-cell protease becomes
inactive upon loss of membrane integrity and leakage into the culture medium.
A second, luminogenic cell-impermeant peptide substrate (AAF-aminoluciferin)
is used to measure dead-cell protease activity, which is released from cells that
have lost membrane integrity. The liberated aminoluciferin product is measured
as “glow type” luminescence generated by Ultra-Glo Recombinant Luciferase
provided in the assay reagent.
The MultiTox-Glo Assay gives ratiometric, inversely correlated measures of
cell viability and cytotoxicity, which correlate with established methods for
measuring viability and cytotoxicity. The ratio of viable cells to dead cells is
independent of cell number and therefore can be used to normalize data.
Having complementary cell viability and cytotoxicity measures reduces errors
associated with pipetting and cell clumping, as well as serving as an internal
control to allow identification of errors resulting from chemical interference
from test compounds or media components.
Features:
• Measure the Number of Live Cells and Dead Cells in Culture:
Sequential-reagent-addition assay with a homogeneous “add-mix-measure”
protocol.
• Normalize Data with a Built-In Internal Control: The ratio of the
number of live cells/number of dead cells is independent of cell number
and can be used to normalize data. This normalization makes results more
comparable well-to-well, plate-to-plate and day-to-day.
• Immediately Identify More False-Positives and False-Negatives:
Independent cell viability and cytotoxicity measurements serve as controls
for each other. If test compounds interfere with one assay chemistry, the
other serves as an internal control.
• Improve your Data: Reduce statistical probability of false-positives (or
false-negatives), and eliminate fluorescence interference issues by luminescence
readout.
Storage Conditions: Store at –20°C, protected from light.
Protocol Part#
MultiTox-Glo Multiplex Cytotoxicity Assay Technical Bulletin TB358
For complete and up-to-date product information visit: www.promega.com/catalog