2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Cell Signaling<br />
CellTiter 96 ® CellTiter 96 AQueous Non-Radioactive Cell<br />
Proliferation Assay (MTS)<br />
® AQueous Non-Radioactive Cell<br />
Proliferation Assay (MTS)<br />
Product Size Cat.# Price ($)<br />
CellTiter 96 ® AQueous Non-Radioactive Cell 1,000 assays G5421 394.00<br />
Proliferation Assay<br />
5,000 assays G5430 1124.00<br />
Available Separately<br />
50,000 assays G5440 Pls. Enq.<br />
CellTiter 96 ® Product Size Cat.# Price ($)<br />
CellTiter 96<br />
AQueous MTS Reagent Powder 1 g G1111 1364.00<br />
For Laboratory Use.<br />
250 mg G1112 424.00<br />
® AQueous Non-Radioactive Cell 1,000 assays G5421 394.00<br />
Proliferation Assay<br />
5,000 assays G5430 1124.00<br />
Available Separately<br />
50,000 assays G5440 Pls. Enq.<br />
CellTiter 96 ® AQueous MTS Reagent Powder 1 g G1111 1364.00<br />
For Laboratory Use.<br />
250 mg G1112 424.00<br />
Description: The CellTiter 96 ® AQueous Non-Radioactive Cell Proliferation<br />
Assay is a homogeneous, colorimetric method for determining the number<br />
of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The<br />
CellTiter 96 ® AQueous Assay is composed of solutions of a novel tetrazolium<br />
compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulfophenyl)-2H-tetrazolium,<br />
inner salt; MTS] and an electron coupling reagent<br />
(phenazine methosulfate) PMS. MTS is bioreduced by cells into a formazan<br />
product that is soluble in tissue culture medium. The absorbance of the<br />
formazan product at 490nm can be measured directly from 96-well assay<br />
plates without additional processing. The conversion of MTS into the aqueous<br />
soluble formazan product is accomplished by dehydrogenase enzymes found in<br />
metabolically active cells. The quantity of formazan product as measured by the<br />
amount of 490nm absorbance is directly proportional to the number of living<br />
cells in culture.<br />
If you currently use a [ 3H]-thymidine incorporation assay, addition of the<br />
combined MTS/PMS solution can be substituted for [ 3H]-thymidine at the time<br />
point in the assay when the pulse of radioactive thymidine is usually added.<br />
Data from proliferation bioassays comparing the CellTiter 96 ® AQueous Assay<br />
and [ 3H]-thymidine incorporation show similar results.<br />
CellTiter 96 ® AQueous MTS Reagent Powder is a novel tetrazolium<br />
compound for use in colorimetric assays for determining the number of viable<br />
cells in proliferation, cytotoxicity or chemosensitivity assays. It is provided in<br />
powdered form.<br />
Features:<br />
• Easy to Use: Combine provided MTS and PMS solutions, add to cells,<br />
incubate and read absorbance.<br />
• Fast: Perform assay in a 96-well plate with no washing or cell harvesting.<br />
Eliminate solubilization steps because the MTS formazan product is soluble<br />
in tissue culture medium.<br />
• Nonradioactive: Requires no scintillation cocktail or radioactive waste<br />
disposal (unlike [ 3 Description: The CellTiter 96<br />
H]-thymidine).<br />
• Flexible: Plates can be read and returned to incubator for further color<br />
development (unlike MTT).<br />
• Safe: Requires no volatile organic solvent to solubilize the formazan<br />
product (unlike MTT).<br />
® AQueous Non-Radioactive Cell Proliferation<br />
Assay is a homogeneous, colorimetric method for determining the number<br />
of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The<br />
CellTiter 96 ® AQueous Assay is composed of solutions of a novel tetrazolium<br />
compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulfophenyl)-2H-tetrazolium,<br />
inner salt; MTS] and an electron coupling reagent<br />
(phenazine methosulfate) PMS. MTS is bioreduced by cells into a formazan<br />
product that is soluble in tissue culture medium. The absorbance of the<br />
formazan product at 490nm can be measured directly from 96-well assay<br />
plates without additional processing. The conversion of MTS into the aqueous<br />
soluble formazan product is accomplished by dehydrogenase enzymes found in<br />
metabolically active cells. The quantity of formazan product as measured by the<br />
amount of 490nm absorbance is directly proportional to the number of living<br />
cells in culture.<br />
If you currently use a [ 3H]-thymidine incorporation assay, addition of the<br />
combined MTS/PMS solution can be substituted for [ 3H]-thymidine at the time<br />
point in the assay when the pulse of radioactive thymidine is usually added.<br />
Data from proliferation bioassays comparing the CellTiter 96 ® AQueous Assay<br />
and [ 3H]-thymidine incorporation show similar results.<br />
CellTiter 96 ® AQueous MTS Reagent Powder is a novel tetrazolium<br />
compound for use in colorimetric assays for determining the number of viable<br />
cells in proliferation, cytotoxicity or chemosensitivity assays. It is provided in<br />
powdered form.<br />
Features:<br />
• Easy to Use: Combine provided MTS and PMS solutions, add to cells,<br />
incubate and read absorbance.<br />
• Fast: Perform assay in a 96-well plate with no washing or cell harvesting.<br />
Eliminate solubilization steps because the MTS formazan product is soluble<br />
in tissue culture medium.<br />
• Nonradioactive: Requires no scintillation cocktail or radioactive waste<br />
disposal (unlike [ 3H]-thymidine). • Flexible: Plates can be read and returned to incubator for further color<br />
development (unlike MTT).<br />
• Safe: Requires no volatile organic solvent to solubilize the formazan<br />
product (unlike MTT).<br />
Storage Conditions: For long-term storage, store MTS and PMS Solutions at<br />
–20°C, protected from from light.<br />
Protocol Part#<br />
CellTiter 96 ® Protocol Part#<br />
CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay<br />
Technical Bulletin<br />
TB169<br />
® AQueous Non-Radioactive Cell Proliferation Assay<br />
Technical Bulletin<br />
TB169<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
CellTiter 96 ® CellTiter 96 Non-Radioactive Cell<br />
Proliferation Assay (MTT)<br />
® Non-Radioactive Cell<br />
Proliferation Assay (MTT)<br />
Product Size Cat.# Price ($)<br />
CellTiter 96 ® Product Size Cat.# Price ($)<br />
CellTiter 96 Non-Radioactive Cell<br />
1,000 assays G4000 371.00<br />
Proliferation Assay<br />
For Laboratory Use.<br />
5,000 assays G4100 1085.00<br />
® Non-Radioactive Cell<br />
1,000 assays G4000 371.00<br />
Proliferation Assay<br />
For Laboratory Use.<br />
5,000 assays G4100 1085.00<br />
Description: The CellTiter 96 ® Assay is a collection of qualified reagents that<br />
provide a convenient method of determining viable cell number. This assay is a<br />
modification of the MTT assay method described by Mosmann and incorporates<br />
several improvements to the method that address previous technical problems<br />
including: 1) serum protein precipitation caused by adding organic solvent; 2)<br />
interference by phenol red; 3) incomplete solubilization of the formazan crystals<br />
resulting in lower sensitivity; and 4) stability of the colored product.<br />
Perform the CellTiter 96 ® Assay by adding a premixed, optimized Dye Solution<br />
to culture wells of a 96-well plate, usually containing various concentrations<br />
of growth factor or test substance. During a 4-hour incubation, living cells<br />
convert the MTT tetrazolium component of the Dye Solution into a formazan<br />
product. If you currently use a [ 3H]-thymidine incorporation assay, the addition<br />
of Dye Solution can be substituted for the pulse of radioactive thymidine at the<br />
time point in the assay when the pulse of [ 3H]-thymidine is usually added. The<br />
Solubilization/Stop Solution is then added to the culture wells to solubilize the<br />
formazan product, and the absorbance at 570nm is recorded using a 96-well<br />
plate reader. Direct comparison between [ 3 Description: The CellTiter 96<br />
H]-thymidine incorporation and<br />
tetrazolium conversion have demonstrated less than a 5% difference between<br />
the two assays for determination of growth factor content of several samples.<br />
Features:<br />
• Gain Sensitivity: Detect as few as 1,000 cells/well with a 96-well plate<br />
reader. Greater sensitivity than the neutral red assay procedure.<br />
• Use a Variety of Cells: Assay mammalian, plant and yeast cells.<br />
• Non-Radioactive: Requires no scintillation cocktail or radioactive waste<br />
disposal.<br />
• Save Time: Perform the assay in a 96-well plate with no washing steps,<br />
no cell harvesting and no scintillation counting.<br />
• Adapt to Your Needs: Follow either a 4-hour or overnight protocol.<br />
• Convenient: Requires no weighing or mixing of dye components.<br />
® Assay is a collection of qualified reagents that<br />
provide a convenient method of determining viable cell number. This assay is a<br />
modification of the MTT assay method described by Mosmann and incorporates<br />
several improvements to the method that address previous technical problems<br />
including: 1) serum protein precipitation caused by adding organic solvent; 2)<br />
interference by phenol red; 3) incomplete solubilization of the formazan crystals<br />
resulting in lower sensitivity; and 4) stability of the colored product.<br />
Perform the CellTiter 96 ® Assay by adding a premixed, optimized Dye Solution<br />
to culture wells of a 96-well plate, usually containing various concentrations<br />
of growth factor or test substance. During a 4-hour incubation, living cells<br />
convert the MTT tetrazolium component of the Dye Solution into a formazan<br />
product. If you currently use a [ 3H]-thymidine incorporation assay, the addition<br />
of Dye Solution can be substituted for the pulse of radioactive thymidine at the<br />
time point in the assay when the pulse of [ 3H]-thymidine is usually added. The<br />
Solubilization/Stop Solution is then added to the culture wells to solubilize the<br />
formazan product, and the absorbance at 570nm is recorded using a 96-well<br />
plate reader. Direct comparison between [ 3H]-thymidine incorporation and<br />
tetrazolium conversion have demonstrated less than a 5% difference between<br />
the two assays for determination of growth factor content of several samples.<br />
Features:<br />
• Gain Sensitivity: Detect as few as 1,000 cells/well with a 96-well plate<br />
reader. Greater sensitivity than the neutral red assay procedure.<br />
• Use a Variety of Cells: Assay mammalian, plant and yeast cells.<br />
• Non-Radioactive: Requires no scintillation cocktail or radioactive waste<br />
disposal.<br />
• Save Time: Perform the assay in a 96-well plate with no washing steps,<br />
no cell harvesting and no scintillation counting.<br />
• Adapt to Your Needs: Follow either a 4-hour or overnight protocol.<br />
• Convenient: Requires no weighing or mixing of dye components.<br />
Storage Conditions: Store Dye Solution at –20°C and Solubilization/Stop<br />
Solution at room temperature.<br />
Protocol Part#<br />
CellTiter 96 ® Protocol Part#<br />
CellTiter 96 Non-Radioactive Cell Proliferation Assay Technical<br />
Bulletin<br />
TB112<br />
® Non-Radioactive Cell Proliferation Assay Technical<br />
Bulletin<br />
TB112<br />
37<br />
2<br />
Cell Health Assays<br />
Section<br />
Contents<br />
Table of<br />
Contents
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