2012 Promega catalogue

Life
Science
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2012
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Cell Signaling
36
Fluorescent Cell Viability Assay
Product Size Cat.# Price ($)
CellTiter-Fluor Cell Viability Assay 10 ml G6080 201.00
For Laboratory Use.
5 × 10 ml G6081 745.00
2 × 50 ml G6082 1115.00
Description: The CellTiter-Fluor Cell Viability Assay is a nonlytic, singlereagent-addition
fluorescence assay that measures the relative number of
viable cells in a population. The assay is based on measurement of a conserved
and constitutive protease activity within live cells and therefore serves as a
biomarker of cell viability. The live-cell protease activity is restricted to intact
viable cells and is measured using a fluorogenic, cell-permeant, peptide
substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved
by the live-cell protease activity to generate a fluorescent signal proportional to
the number of living cells. The live-cell protease becomes inactive upon loss of
membrane integrity and leakage into the surrounding culture medium.
The CellTiter-Fluor Assay also can be used in a single-well, sequential,
multiplex format with other downstream assay chemistries to normalize data
by cell number. Data from the assay can serve as an internal control and allow
identification of errors resulting from cell clumping or compound cytotoxicity.
The assay is compatible with many Promega luminescence assays or spectrally
distinct fluorescence assay methods, such as measuring caspase activation,
reporter gene expression or orthogonal measures of viability.
Features:
• Obtain Better Data from Every Well: The assay can be performed in
multiplex with many Promega luminescence assays or spectrally distinct
fluorescence assays.
• Normalize Data for Cell Number: Normalizing data for live-cell number
makes results more comparable well-to-well, plate-to-plate, day-to-day.
• Save on Cell Culture Costs: Multiplexing assays in the same well eliminates
parallel plate processing, thus reducing cell culture costs.
Storage Conditions: Store at –20°C.
Protocol Part#
CellTiter-Fluor Cell Viability Assay Technical Bulletin TB371
CellTiter 96 ® AQ ueous One Solution Cell
Proliferation Assay (MTS)
Product Size Cat.# Price ($)
CellTiter 96 ® AQueous One Solution Cell 200 assays G3582 107.00
Proliferation Assay
1,000 assays G3580 409.00
For Laboratory Use.
5,000 assays G3581 1155.00
Description: The CellTiter 96 ® AQ ueous One Solution Cell Proliferation Assay is
a colorimetric method for determining the number of viable cells in proliferation,
cytotoxicity or chemosensitivity assays. The CellTiter 96 ® AQ ueous One
Solution Reagent contains a tetrazolium compound [3-(4,5-dimethylthiazol-
2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt;
MTS] and an electron coupling reagent (phenazine ethosulfate; PES). PES has
enhanced chemical stability, which allows it to be combined with MTS to form
a stable solution. The CellTiter 96 ® AQ ueous Assay uses phenazine methosulfate
(PMS) as the electron coupling reagent, and PMS Solution and MTS Solution
are supplied separately. PES has enhanced chemical stability, which allows it to
be combined with MTS to form a stable solution.
Assays are performed by adding a small amount of the CellTiter 96 ® AQ ueous
One Solution Reagent directly to culture wells, incubating for 1–4 hours and
then recording absorbance at 490nm with a 96-well plate reader. The quantity
of formazan product as measured by the amount of 490nm absorbance is
directly proportional to the number of living cells in culture.
If you currently use a [ 3 H]-thymidine incorporation assay, addition of the
CellTiter 96 ® AQ ueous One Solution Reagent can be substituted for the pulse
of [ 3 H]-thymidine at the time point in the assay when the pulse of radioactive
thymidine is usually added. Previous bioassay data comparing [ 3 H]-thymidine
incorporation to the MTS-based CellTiter 96 ® AQ ueous Assay and the original
MTT-based CellTiter 96 ® Assay demonstrate that tetrazolium reagents can be
substituted for [ 3 H]-thymidine incorporation.
Features:
• Simplify Colorimetric Viability Assays: “Add-incubate-measure” format
enables design of homogeneous high-throughput screening assays.
• Use a Single Solution: Use as a single solution, filter sterilized and ready
to add to assay plates (unlike MTT).
• Perform Fewer Steps: Perform the assay in 96-well plates with no washing
or cell harvesting. Also eliminates solubilization steps normally required
for MTT assays.
• Gain Flexibility: Plates can be read and returned to incubator for further
color development (unlike MTT).
• Avoid Organic Solvents: Requires no volatile organic solvent to solubilize
the formazan product (unlike MTT).
• Non-Radioactive: Requires no scintillation cocktail or radioactive waste
disposal (unlike [ 3 H]-thymidine incorporation assays).
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C, protected from light.
Protocol Part#
TB245
CellTiter 96 ® AQ ueous One Solution Cell Proliferation Assay System
Technical Bulletin
Corrected Ab. 490nm
2.00
1.50
1.00
0.50
Comparison of MTS and [ 3 H]thymidine Assays
Proliferation of HT-2 Cells Stimulated with GM-CSF
0.00
0
0 .01 .04 .16 .62 2.5 10
ng/ml GM-CSF
For complete and up-to-date product information visit: www.promega.com/catalog
MTS
[ 3 H]-Thy
Measurement of GM-CSF-stimulated proliferation in HT-2 cells using
the CellTiter 96 ® AQ ueous Cell Proliferation Assay and a [ 3 H]thymidine
incorporation assay. Similar results were obtained with both assays.
16
12
8
4
CPM (x 10 -3 )
2372MA08_8A